Sustainability has become a priority in the last decades. If we consider rural regions, agritourism, an activity strongly related to local communities, represents an opportunity to ensure the sustainability of rural settlements and of the environment, and at the same time an innovative and diversifying possibility for the economic support of rural settlements. Many studies have shown that the sustainability trend of the current period can be achieved by combining rural and local resources and traditional products specific to the rural regions with tourist activities, the challenge being the revitalization of rural economy. A large number of Romanian mountain rural regions can provide opportunities for sustaining agritourism activity; some of them may succeed to capitalize on the niche characteristic of agritourism, and through appropriate and effective strategies to move from the pioneering stage to an economically and socially valuable chance for improving the living conditions from rural area and finally to ensure sustainable development of rural communities. This paper begins from the importance of agritourism for rural regions. It aims at identifying the current situation of the agritourism activity in three regions and at developing proposals that are based strictly on the specific needs of the regions. In our opinion, the implementation of these specific actions would represent future challenges and at the same time ways of supporting the sustainable development of agritourism activities and of local settlements.

This study was performed in order to determine whether human isolated probiotic bacteria can be effective in reducing Campylobacter jejuni infection of chicken intestinal cells, in vitro, and in decreasing its colonization abilities within the chicken gut. Our results show that the probiotic strains Lactobacillus paracasei J. R, L. rhamnosus 15b, L. lactis Y, and L. lactis FOa had a significant effect on C. jejuni invasion of chicken primary cells, with the strongest inhibitory effect detected when a combination of four was administered. In regard to the in vivo effect, using all four strains in one combination prevented mucus colonization in the duodenum and cecum. Moreover, the pathogen load in the lumen of these two compartments was significantly reduced. When probiotics were introduced during the early growth period, the presence of the pathogen in feces was increased (p>0.05), but when they were given during the last week of growth, there was no significant effect. In conclusion, our data indicate that these four new probiotic strains are able to cause modifications in the chicken intestinal mucosa and can reduce the ability of C. jejuni to invade, in vitro, and to colonize, in vivo. These probiotics are now proven to be effective even when introduced in broiler's feed 7 days before slaughter, which makes them cost-effective for the producers.

BackgroundGiven the serious threats posed to terrestrial ecosystems by industrial contamination, environmental monitoring is a standard procedure used for assessing the current status of an environment or trends in environmental parameters. Measurement of metal concentrations at different trophic levels followed by their statistical analysis using exploratory multivariate methods can provide meaningful information on the status of environmental quality. In this context, the present paper proposes a novel chemometric approach to standard statistical methods by combining the Block clustering with Partial least square (PLS) analysis to investigate the accumulation patterns of metals in anthropized terrestrial ecosystems. The present study focused on copper, zinc, manganese, iron, cobalt, cadmium, nickel, and lead transfer along a soil-plant-snai food chain, and the hepatopancreas of the Roman snail (Helix pomatia) was used as a biological end-point of metal accumulation.ResultsBlock clustering deliniates between the areas exposed to industrial and vehicular contamination. The toxic metals have similar distributions in the nettle leaves and snail hepatopancreas. PLS analysis showed that (1) zinc and copper concentrations at the lower trophic levels are the most important latent factors that contribute to metal accumulation in land snails; (2) cadmium and lead are the main determinants of pollution pattern in areas exposed to industrial contamination; (3) at the sites located near roads lead is the most threatfull metal for terrestrial ecosystems.ConclusionThere were three major benefits by applying block clustering with PLS for processing the obtained data: firstly, it helped in grouping sites depending on the type of contamination. Secondly, it was valuable for identifying the latent factors that contribute the most to metal accumulation in land snails. Finally, it optimized the number and type of data that are best for monitoring the status of metallic contamination in terrestrial ecosystems exposed to different kinds of anthropic polution.

In our days consumers are interested and visibly reoriented towards natural/local/organic products. Starting from this specificity, the development of rural tourism can come as a possible sustainable response to be implemented in many of the rural areas, such an example being the Moieciu area subject to our analysis. The main objective of this paper is to achieve an analysis of rural tourism activity’s development stage from Moieciu area, from the tourist’s perspective, using case study method and as “main tool of research” the questionnaire. Thus, several research-oriented goals followed are: establishing the arguments for practicing tourism in rural areas; describing the development of Bran-Moieciu area from economic, agricultural and tourist point of view; identifying the reasons for which Moieciu area is a place where tourism can ensure sustainable development. The measure of appreciation of this area by the tourist is analyzed through a face-to-face questionnaire, having as purpose to identify: the capitalization level of local resources through rural tourism activities; the extent to which local resources are present in the product consumed by tourists; the satisfaction degree and expectations regarding the tourist product; the connection between tourism and area development in terms of satisfaction. Based on the information obtained some proposals/possible directions will be proposed to transform the area into “a possible model of sustainable rural tourism development”.

Objectives In this study we have investigated the in vitro and in vivo virulence characteristics of a new T6SS positive Campylobacter jejuni chicken isolate (SV12) originating from a poultry population in North Romania. A detailed phenotypic characterization was performed and compared to the T6SS negative C. jejuni 81–176 wild strain. Results Our results indicate that the significantly higher capacity to attach and invade HCT-8 cells of C. jejuni SV12 isolate is associated with increased motility, increased resistance to bile salts and serum resistance, when compared to C. jejuni strain 81–76. Mice infected with the SV12 isolate showed statistically higher levels of colonization at both 7- and 14-days post-inoculation and in the stomach, caecum, duodenum and large intestine. Infection with the SV12 strain induced a stronger immune response as the gene transcript levels of IL-17, TNFα and IFNγ were more pronouncedly up-regulated compared to the C. jejuni strain 81–176. The present study showed that the new isolate SV12 had an enhanced virulence capacity compared to the wild strain which was evident in vivo as well. This work also provides an insight on the colonization pattern and host immune response differences between T6SS positive and T6SS negative C. jejuni .