Oct4 is essential to maintain pluripotency and has a pivotal role in establishing the germline. Its DNA-binding POU domain was recently found to bind motifs with methylated CpG elements normally associated with epigenetic silencing. However, the mode of binding and the consequences of this capability has remained unclear. Here, we show that Oct4 binds to a compact palindromic DNA element with a methylated CpG core (CpGpal) in alternative states of pluripotency and during cellular reprogramming towards induced pluripotent stem cells (iPSCs). During cellular reprogramming, typical Oct4 bound enhancers are uniformly demethylated, with the prominent exception of the CpGpal sites where DNA methylation is often maintained. We demonstrate that Oct4 cooperatively binds the CpGpal element as a homodimer, which contrasts with the ectoderm-expressed POU factor Brn2. Indeed, binding to CpGpal is Oct4-specific as other POU factors expressed in somatic cells avoid this element. Binding assays combined with structural analyses and molecular dynamic simulations show that dimeric Oct4-binding to CpGpal is driven by the POU-homeodomain whilst the POU-specific domain is detached from DNA. Collectively, we report that Oct4 exerts parts of its regulatory function in the context of methylated DNA through a DNA recognition mechanism that solely relies on its homeodomain.

Transmission line detection is a magic weapon to protect power safety, and it plays an irreplaceable role in stabilizing electricity use. However, the detection of transmission lines contains complex objects and rich content, which puts forward a new test for the accurate identification and power consumption of the monitoring. The advantages of Zigbee wireless communication technology are low energy consumption, simple operation, and strong cost control. It is a key to cracking the high energy consumption of transmission line detection. Edge computing is a supplement to cloud computing. It has the characteristics of low bandwidth and low latency, which is conducive to cracking the complex scenarios of wireless monitoring. This article was aimed at studying and designing a monitoring system for transmission lines to ensure the orderly operation of remote monitoring of transmission lines. This paper proposes to use Zigbee wireless communication as the core and integrate edge computing to construct a wireless transmission line sensing system to form a remote transmission line detection system. This paper builds a detection system, systematically tested the system’s energy consumption, anti-interference, fault monitoring recall, and system accuracy, and evaluated the overall operation of the system as a whole. The experimental results in this paper show that the monitoring and attraction of transmission lines can effectively reduce the risk of circuit failure by 20%.

Somatic cell reprogramming and oncogenic transformation share surprisingly similar features, yet transformed cells are highly resistant to reprogramming. There must be barriers that block transformed cells from reprogramming, but the nature of those barriers is unclear. In this study, we generated a systematic panel of transformed mouse embryonic fibroblasts (MEFs) using a variety of oncogenic transgenes, and discovered transformed cell lines that remain compatible with reprogramming when transfected with Oct4/Sox2/Klf4/Myc. By comparing the reprogramming-capable and incapable transformed lines we identified multiple stages of failure in the reprogramming process. Some transformed lines failed very early, whilst other lines seemed to progress through a normal-looking reprogramming process. Finally, we show that MEK inhibition overcomes one critical reprogramming barrier by indirectly suppressing a hyperactive epigenetic state in some of the transformed cells. This study reveals that the barriers underlying resistance to reprogramming vary between the different transformation methods.