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Phosphorylation of nucleosides with sodium cyclo-triphosphate (P3m) was studied under various conditions (mixing ratio of P3m to nucleoside, pH, reaction temperature and time). 1) Adenosine, cytidine, guanosine, and uridine were easily phosphorylated by P3m to form selectively nucleoside 2′-monophosphates, 3′-monophosphates, and 2′,3′-cyclic monophosphates. On the other hand, 2′-deoxyadenosine, 3′-deoxyadenosine, 2′-deoxycytidine, and thymidine could not be phosphorylated by P3m. 2) The phosphorylation of adenosine, cytidine, guanosine, and uridine varied strongly, depending on the reaction conditions; mixing ratio, pH, reaction temperature and time. Under conditions of a high mixing ratio of P3m to nucleoside (5:1–10:1), high pH (12–14), high temperature (70–100 °C) for a short time (1–2d) or low temperature (room temperature) for a long time (70–150 d), nucleoside 2′- and 3′-monophosphate could be obtained in high yield (about 50–100%). 3) At the initial stage of the phosphorylation reactions of adenosine, cytidine, and guanosine with P3m, nucleoside 2′,3′-cyclic monophosphates were formed, though a small amount (about 1–3%), but in the course of the reaction for a long time, they were hydrolyzed to nucleoside 2′- and 3′-monophosphates. 4) 2′-deoxyadenosine, 3′-deoxyadenosine, 2′-deoxycytidine, and thymidine could not be phosphorylated by P3m. This means that the presence of hydroxyl groups at the 2′- and 3′-positions of the ribose was necessary for the phosphorylation of nucleosides with P3m. 5) Phosphorylation of nucleosides with short-chain phosphates (ortho-, pyro-, and triphosphate) could not be observed at all. 6) The mechanism of formation of nucleoside 2′-monophosphates, 3′-monophosphates, and 2′,3′-cyclic monophosphates in the phosphorylation of nucleosides with P3m was discussed.

The intercalation of polyamine into α-zirconium phosphate (α-ZrP) was examined at room temperature and 80 °C. In triethylenetetramine (3E4A), tetraethylenepentamine (4E5A), and pentaethylenehexamine (5E6A) the temperature of the reactions influenced the rate of intercalation, but did not effect the structure of the intercalation compound. On the other hand, the ratios of the constituent phases in the N,N′-bis(3-aminopropyl)-1,3-propanediamine (3P4A) intercalated solid were greatly influenced by the reaction temperature, and two phases with the different interlayer distances could be formed by controlling it. In the phase with an interlayer distance of d = 20 Å, obtained predominantly at 80 °C, 3P4A was present as a monolayer of extended molecules in the interlayer region, in which the longitudinal axis of 3P4A was inclined at ca. 60° to the α-ZrP layers. In the phase with d = 17 Å, obtained at room temperature, 3P4A existed as a bent form. These polyamine intercalated solids drastically adsorbed acetic acid, formaldehyde, acetaldehyde, and cinnamaldehyde.

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