Summary 1.Regional above-ground biomass estimates for tropical moist forests remain highly inaccurate mostly because they are based on extrapolations from a few plots scattered across a limited range of soils and other environmental conditions. When such conditions impact biomass, the estimation is biased. The effect of soil types on biomass has especially yielded controversial results. 2. We investigated the relationship between above-ground biomass and soil type in undisturbed moist forests in the Central African Republic. We tested the effects of soil texture, as a surrogate for soil resources availability and physical constraints (soil depth and hydromorphy) on biomass. Forest inventory data were collected for trees ‡20 cm stem diameter in 2754 0.5 ha plots scattered over 4888 km 2 . The plots contained 224 taxons, of which 209 were identified to species. Soil types were characterized from a 1:1 000 000 scale soil map. Species-specific values for wood density were extracted from the CIRAD's data base of wood technological properties. 3. We found that basal area and biomass differ in their responses to soil type, ranging from 17.8 m 2 ha )1 (217.5 t ha ). While shallow and hydromorphic soils support forests with both low stem basal area and low biomass, forests on deep resource-poor soils are typically low in basal area but as high in biomass as forests on deep resource-rich soils. We demonstrated that the environmental filtering of slow growing dense-wooded species on resource-poor soils compensates for the low basal area, and we discuss whether this filtering effect is due to low fertility or to low water reserve. 4. Synthesis. We showed that soil physical conditions constrained the amount of biomass stored in tropical moist forests. Contrary to previous reports, our results suggest that biomass is similar on resource-poor and resource-rich soils. This finding highlights both the importance of taking into account soil characteristics and species wood density when trying to predict regional patterns of biomass. Our findings have implications for the evaluation of biomass stocks in tropical forests, in the context of the international negotiations on climate change.

In carbon cycle research, tree wood density is used to compute forest carbon stock and assess the role of forests in mitigating climate change (Pan et al., 2011;Vieilledent et al., 2016) or evaluate the impact of deforestation on climate (Achard et al., 2014). In community ecology, wood density is a proxy for species performance (Lachenbruch and McCulloh, 2014), reflecting a trade-off between growth potential and mortality risk from biomechanical or hydraulic failure (Díaz et al., 2016). Fast-growing, short-lived species tend to have a lower wood density, while slow-growing, long-lived species tend to have a higher wood density (Chave et al., 2009;Greenwood et al., 2017). In wood technology, most physical and mechanical properties of wood (e.g., strength, stiffness, porosity, heat transmission, yield of pulp per unit volume) are closely related to wood density (Sallenave, 1955;Thibaut et al., 2001;Shmulsky and Jones, 2011). This explains why wood density has been commonly measured in forestry institutes, where wood was principally studied for construction or paper making.Wood density was originally measured at ambient air moisture after air drying (Glass and Zelinka, 2010), but is now measured at a fixed moisture content, such as 15% or the international standard of 12% (Sallenave, 1955). In temperate countries, construction wood is at equilibrium with ambient air at an average moisture close to 12%. Wood density at 12% moisture is the ratio between the mass

The Vibrio splendidus clade has previously been associated with epidemic outbreaks of various aquatic animals, as in the case of the cupped oyster, Crassostrea gigas. To investigate whether involved strains could present a clonal origin and to identify possible alternative background carriage animals or zooplankton, a large epidemiological survey was conducted on isolates of the splendidus clade. For this purpose, Vibrio strains were isolated from various samples including oysters, mussels, sediments, zooplankton, and sea water on the basis of a North/South gradient of the European sea water zone (Ireland, The Netherlands, France, Italy, and Spain). A total of 435 isolates were successfully associated to the V. splendidus clade using real time polymerase chain reaction with 16S specific primers and probes. A multiple-locus variable-number tandem-repeat analysis (VNTR) was conducted on all isolates based on a multiplex PCR-VNTR with a set of primer pairs designed from the V. tasmaniensis LGP32 genome. Preliminary validation of the primers on a set of collection strains from the V. splendidus clade confirmed that the former V. splendidus-related LGP32 and relative strains were related to V. tasmaniensis rather than to the type strain V. splendidus LMG 4042. The VNTR analysis was then successfully conducted on 335 isolates which led to the characterization of 87 different profiles. Our results showed that (1) the high diversity of VNTR did not enlighten significant correlation between a specific pattern and the origin of collected samples. However, populations isolated from animal samples tend to differ from those of the background environment; (2) oyster mortality events could not be linked to the clonal proliferation of a particular VNTR type. However, few different patterns seemed successively associated with samples collected during peaks of oyster's mortality. (3) Finally, no correlation could be seen between specific VNTR patterns and sequence phylogeny of the virulence factors vsm and ompU that were detected among strains isolated during as well as outside mortality events. These results, combined with incongruence observed between the ompU and vsm phylogenetic trees, suggested both large diffusion of strains and massive lateral gene transfer within the V. splendidus clade.

h i g h l i g h t sDifferent surface treatments are applied on oil palm shell (OPS). Effects of treated OPS on physico-mechanical properties of concrete are studied. Lime treatment increases the mechanical properties of OPS concrete. Sodium silicate treatment has not enhanced the bond between cement paste and OPS. Prewetting OPS and PVA treatment reduces the shrinkage and thermal conductivity. The overuse of natural aggregates for construction causes many environmental problems. In light of their environmental impact, the discussion has increasingly focused on using alternative plant-based materials and processes such as oil palm shells (OPS). However, previous studies show that OPS have a weak adhesion with cement paste, which results in a decrease in the physical and mechanical properties of OPS concretes. One of the solutions for this problem is to carry out a surface treatment on OPS before using them in concrete. This study has examined the influence of five treatments on the physical and mechanical properties of concrete: treatment with lime (CH), sodium silicate (SS), polyvinyl alcohol (PVA), heat treatment (TH) and OPS saturation (SAT). Lime treatment (CH) on OPS showed good improvement in the mechanical properties of concrete, compared to untreated OPS.