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hydrogen production using green microalgae as an approach to operate a small proton exchange membrane fuel cell. International Journal of Hydrogen Energy, Elsevier, 2011, 36, pp.4089-4093. hal-00652813 Biohydrogen production using green microalgae as an approach to operate a small Proton Exchange Membrane Fuel Cell AbstractIn this paper the wild-type of Chlorella sorokiniana, a green microalga isolated fromAlgerian Sahara soil, is tested for it ability to produce hydrogen in a 500 ml photobioreactor coupled to a small Proton Exchange Membrane Fuel Cell (PEMFC). The strain grown in heterotrophically conditions (Tris-Acetate-Phospate medium) under continuous light is transferred, in medium without sulfur to make anaerobiosis and activate the reversible hydrogenase, inducing the hydrogen production process. The evolution of hydrogen and oxygen concentrations in the photobioreactor during the sulfurdeprived conditions is measured. The difference of total carbohydrates amount and cell morphology at the beginning and the end of experience is also studied. 1/13 coupled system show that the produced biohydrogen can be used to operate a PEM FuelCell with good performances under standard conditions.

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