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BackgroundSalt stress hinders the growth of plants and reduces crop production worldwide. However, different plant species might possess different adaptive mechanisms to mitigate salt stress. We conducted a detailed pathway analysis of transcriptional dynamics in the roots of Medicago truncatula seedlings under salt stress and selected a transcription factor gene, MtCBF4, for experimental validation.ResultsA microarray experiment was conducted using root samples collected 6, 24, and 48 h after application of 180 mM NaCl. Analysis of 11 statistically significant expression profiles revealed different behaviors between primary and secondary metabolism pathways in response to external stress. Secondary metabolism that helps to maintain osmotic balance was induced. One of the highly induced transcription factor genes was successfully cloned, and was named MtCBF4. Phylogenetic analysis revealed that MtCBF4, which belongs to the AP2-EREBP transcription factor family, is a novel member of the CBF transcription factor in M. truncatula. MtCBF4 is shown to be a nuclear-localized protein. Expression of MtCBF4 in M. truncatula was induced by most of the abiotic stresses, including salt, drought, cold, and abscisic acid, suggesting crosstalk between these abiotic stresses. Transgenic Arabidopsis over-expressing MtCBF4 enhanced tolerance to drought and salt stress, and activated expression of downstream genes that contain DRE elements. Over-expression of MtCBF4 in M. truncatula also enhanced salt tolerance and induced expression level of corresponding downstream genes.ConclusionComprehensive transcriptomic analysis revealed complex mechanisms exist in plants in response to salt stress. The novel transcription factor gene MtCBF4 identified here played an important role in response to abiotic stresses, indicating that it might be a good candidate gene for genetic improvement to produce stress-tolerant plants.

Here we present, to date, the highest-resolved (~5 years) and most precisely dated Holocene monsoon climate reconstruction for the western Chinese Loess Plateau based on five replicated stalagmite δ 18 O records from Wuya Cave, eastern Gansu, China. Our record suggests the wettest period occurred between 10,500 and 6,600 a BP in this region. After this period, the amplitude of Asian summer monsoon decadal-scale variability progressively increased likely in response to increasing ENSO frequency since the middle Holocene. Our study reveals similar asymmetric centennial-scale double-plunging structures of the 8.2, 5.5, and 2.8 ka events in the western Chinese Loess Plateau, suggesting a possible role of solar activity whose impact was amplified around 8.2 ka BP by the meltwater flood. In contrast, the 4.2 ka event exhibit gradually declining monsoon rainfall with centennial-to decadal-scale fluctuations.