Production of interleukin-1 and tumour necrosis factor from stimulated human monocytes is inhibited by a new series of pyridinyl-imidazole compounds. Using radiolabelled and radio-photoaffinity-labelled chemical probes, the target of these compounds was identified as a pair of closely related mitogen-activated protein kinase homologues, termed CSBPs. Binding of the pyridinyl-imidazole compounds inhibited CSBP kinase activity and could be directly correlated with their ability to inhibit cytokine production, suggesting that the CSBPs are critical for cytokine production.

Abstract. A comprehensive set of accurate atomic data is required for analyses of astrophysical and solar spectra. CHIANTI provides a database of atomic energy levels, wavelengths, radiative data and electron excitation data for ions which are abundant in cosmic plasmas. The most recent electron excitation data have been assessed and stored following the method of Burgess & Tully (1992). The current version is essentially complete for specifying the emission spectrum at wavelengths greater than 50Å. A list of observed lines in the spectral region between 50 and 1100Å has been compiled and compared with the lines predicted by the CHIANTI database. The CHIANTI database reproduces the vast majority of lines observed at these wavelengths. CHIANTI includes IDL (Interactive Data Language) routines to calculate optically thin synthetic spectra for equilibrium conditions. IDL routines to calculate theoretical line intensities required for electron density or temperature diagnostics and emission measure studies are also included. The CHIANTI atomic database and supporting IDL routines are available by anonymous FTP.

Single-nucleotide polymorphisms (SNPs) are the most frequent type of variation in the human genome, and they provide powerful tools for a variety of medical genetic studies. In a large-scale survey for SNPs, 2.3 megabases of human genomic DNA was examined by a combination of gel-based sequencing and high-density variation-detection DNA chips. A total of 3241 candidate SNPs were identified. A genetic map was constructed showing the location of 2227 of these SNPs. Prototype genotyping chips were developed that allow simultaneous genotyping of 500 SNPs. The results provide a characterization of human diversity at the nucleotide level and demonstrate the feasibility of large-scale identification of human SNPs.