A novel hydrothermal site was discovered in March 2007, on the mid-Atlantic ridge during the cruise 'Serpentine'. At a depth of 4100 m, the site 'Ashadze' is the deepest vent field known so far. Smoker samples were collected with the ROV 'Victor 6000' and processed in the laboratory for the enrichment of anaerobic heterotrophic microorganisms under high-temperature and high-hydrostatic pressure conditions. Strain CH1 was successfully isolated and assigned to the genus Pyrococcus, within the Euryarchaeota lineage within the Archaea domain. This organism grows within a temperature range of 80 to 108 1C and a pressure range of 20 to 120 MPa, with optima for 98 1C and 52 MPa respectively. Pyrococcus CH1 represents the first obligate piezophilic hyperthermophilic microorganism known so far. Comparisons of growth yields obtained under hightemperature/high-pressure conditions for relative organisms isolated from various depths, showed clear relationships between depth at origin and responses to hydrostatic pressure.

With certain concerns recently reported on metal-on-metal bearing couples in total hip arthroplasty, this study's objective is to review the current knowledge concerning release of metal ions and its potential consequences. Each metal-on-metal implant presents different tribological properties. The analytical techniques for metals are accurate and the Co ion rates seem acceptable up to 2 μg/L. A delayed type IV hypersensitivity reaction (atypical lymphocytic vasculitis-associated lesion [ALVAL]) may be the source of arthroplasty failure. Idiosyncratic, it remains unpredictable even using cutaneous tests and apparently is rare (0.3%). Today, there are no scientific or epidemiologic data supporting a risk of carcinogenesis or teratogenesis related to the use of a metal-on-metal bearings couple. Solid pseudotumors nearly exclusively are observed with resurfacing procedures, carrying a high annual revision rate in women under 40 years of age, occurring particularly in cases of acetabular malposition and with use of cast molded Cr-Co alloys. Osteolysis manifests through complete and progressive radiolucent lines or through cavitary lesions stemming from ALVAL-type alterations or impingement problems or implant incompatibility. The formation of wear debris exceeding the biological tolerance is possible with implant malposition, subluxation, and jamming of the femoral head in cases of cup deformity. Moreover, each implant presents different metal ion production; assessment of their performance and safety is required before their clinical use. With the knowledge available today, metal-on-metal bearing couples are contraindicated in cases of metal allergies or end stage renal dysfunction and small size resurfacing should cautiously be used.

Purpose The purpose of this study was to assess the fusion rate on CT examinations and to correlate clinically the functional result with the degree of bone fusion in the subtalar joint after posterior arthroscopic subtalar arthrodesis (PASTA). Methods Fourteen cases, from 36 to 84 years old, were retrospectively followed-up for a minimum of one year (range 12-92 months). A CT scan had been systematically performed at the six-month follow-up visit. The CT scans were examined in sagittal 2-mm-thick reformatted slices, measuring the length of the joint surface and the length of the fused portion of the joint space on each image. Results At six months, the average fusion ratio was 39±19% (range 0-69%). Fusion defined by a fusion ratio superior or equal to 33% on the CT scan was observed in 11 cases. One patient had a delayed union and required a revision of fixation. One patient had a bilateral nonunion. Mean average AOFAS score improved from 51±10 to 77±9 at last follow-up.Conclusion Compared to open procedures, the posterior arthroscopic fusion seems to offer a promising alternative. However, our results suggest that the fusion rate following PASTA is not as favourable as reported in previous studies. Factors such as adequate compression and stable fixation provided by the screws together with the surgeons' experience with this demanding technique are of the utmost importance. A 33% CT fusion ratio threshold could accurately discriminate between clinical stability and instability.