The appropriate supply of nutrients in pregnant cows has been associated with the optimal development of foetal tissues, performance of their progeny and their meat quality. The aim of this study was to evaluate supplementation effects of grazing cows in different stages of gestation on skeletal muscle development and performance of the progeny. Thereby, 27 Nellore cows were divided into three groups (n=9 for each group) and their progeny as follows: UNS, unsupplemented during gestation; MID, supplemented from 30 to 180 days of gestation; LATE, supplemented from 181 to 281 days of gestation. The percentage composition of the supplement provided for the matrices was the following: ground corn (26.25%), wheat bran (26.25%) and soya bean meal (47.5%). The supplement was formulated to contain 30% CP. Supplemented matrices received 150 kg of supplement (1 and 1.5 kg/day for cows in the MID and LATE groups, respectively). After birth, a biopsy was performed to obtain samples of skeletal muscle tissue from calves to determine number and size of muscle fibres and for messenger RNA (mRNA) expression analysis. The percentage composition of the supplement provided for the progeny was the following: ground corn grain (30%), wheat bran (30%), soya bean meal (35%) and molasses (5%). The supplement was formulated to contain 25% CP and offered in an amount of 6 g/kg BW. Performance of the progeny was monitored throughout the suckling period. Means were submitted to ANOVA and regression, and UNS, MID and LATE periods of supplementation were compared. Differences were considered at P0.10). Similarly, no differences were observed between calves for nutrient intake (P>0.10). However, greater subcutaneous fat thickness (P=0.006) was observed in the calves of LATE group. The ribeye area (P=0.077) was greater in calves born from supplemented compared with UNS cows. The supplementation of pregnant cows did not affect the muscle fibre size of their progeny (P=0.208). On the other hand, calves born from dams supplemented at mid-gestation had greater muscle fibre number (P=0.093) compared with calves from UNS group. Greater mRNA expression of peroxysome proliferator-activated receptor α (P=0.073) and fibroblast growth factor 2 (P=0.003) was observed in the calves born from MID cows. Although strategic supplementation did not affect the BW of offspring, it did cause changes in carcass traits, number of myofibres, and mRNA expression of a muscle hypertrophy and lipid oxidation markers in skeletal muscle of the offspring.

The objective of the present study was to evaluate the effect of increasing levels of prepartum and postpartum protein supplementation on nutrient intake and digestibility, productive performance and metabolic status of beef heifers on tropical pasture. Twenty-eight pregnant beef heifers with bodyweight and body condition score of 459.7 ± 6.8 kg and 5.8 ± 0.10 respectively, were used. The experiment was performed according to a completely randomised design, with the following four treatments: 0.0, 0.4, 0.8 and 1.2 kg/animal.day of protein supplement containing 25% crude protein. All animals received a mineral mixture ad libitum. The experiment lasted 210 days, divided into three experimental periods. The supplementation level increased the intake of all evaluated nutrients, digestibility of organic matter, neutral detergent fibre corrected for ash and protein, as well as higher average daily gain prepartum and bodyweight of calves at birth (P < 0.05). Protein supplement at the levels evaluated did not affect body condition score (P > 0.05). An interaction effect between treatment × days in relation to calving occurred for concentrations of serum urea nitrogen, total proteins, albumin and globulins (P < 0.05). Supplementation did not affect glucose, triglyceride, non-esterified fatty acids, β-hydroxybutyrate or progesterone (P > 0.05). In conclusion, supply of up to 1.2 kg/day of protein supplement for grazing beef heifers optimises forage intake and average daily gain before calving, and improves the indicators of the protein status.

BackgroundNutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments: control, which received no supplement; supplementation for the last 30 d of gestation (30-d; 3.0 kg/d); supplementation for the last 60 d of gestation (60-d; 1.5 kg/d); or supplementation for the last 90 d of gestation (90-d; 1.0 kg/d). All supplemented treatments received the same total amount of supplement throughout the experiment: 90 kg (20% of crude protein). A second experiment (Experiment 2) was delineated to evaluate the effects of the amounts offered in Experiment 1 on intake and metabolism. Four multiparous pregnant Nellore cows were assigned to a 4 × 4 Latin square design, with periods of 15 d each.ResultsThere was a linear effect of the number of days of supplementation on calving body weight (BW; P < 0.05) and a quadratic effect on BW change from parturition to d 31 post-calving (P < 0.05), with cows on the 60-d strategy losing less BW post-calving. No difference was found in offspring birth BW (P > 0.10). A significant linear effect on interval from parturition to conception (P < 0.05) was observed, with the highest calving to conception interval being observed in the 90-d strategy. The level of supplementation did not affect forage intake or neutral detergent fiber digestibility (P > 0.10). Nitrogen excreted through urine tended to increase linearly with the level of supplementation (P < 0.10).ConclusionProviding 1.5 kg of supplement during the last 60 d of gestation improves cow performance after calving, reducing the magnitude of BW lost, and reduces the number of days from calving to re-conception in the following breeding season compared to the usually recommended period of supplementation of 90 d pre-partum.

A 55-year-old woman was diagnosed with pneumonia and was treated with meropenem; 5 days later she developed epileptic seizures. She had been treated with valproic acid for 16 years to control her epileptic seizures. Her serum valproic acid concentration was low during treatment with meropenem than previously recorded despite an increase of valproic dose. As soon as administration of meropenem was withdrawn, valproic acid concentration increased to previous levels and her seizures stopped. Meropenem decreases valproic acid concentration, and may promote the development of epileptic seizures in previously controlled epileptic patients. The acute lowering of serum valproate produced by meropenem probably precludes their concomitant use.