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Non-selective use of pesticide to increase productivity leaves residues on crops. These pesticides after consumption have a detrimental effect on human health and their intake can lead to various diseases such as kidney damage, neurological disorders, cardiovascular diseases, circulatory and reproductive problems. The recent study was designed to assess the effects of household processing treatments such as acidic solutions (acidic acid and citric acid), alkaline solutions (sodium chloride and sodium carbonate) and biological solutions (radish, ginger, garlic, and lemon extracts) were evaluated for their efficiency for removal of pesticides in spinach by gas chromatography with electron capture detector (GC-ECD). The results showed that pesticide residues were sharply reduced when spinach was exposed to washing treatments. The results showed that the greatest reduction of deltamethrin, cypermethrin, chlorpyrifos and endosulfan with 10% acetic acid was (79.68 ± 0.81%), (89.99 ± 0.12%), (94.21 ± 0.02%) and (70.32 ± 0.25%), respectively of tested washing solutions. The acidic solutions were more effective than alkaline and biological extracts in the removal of pesticide residues. The average reduction in various washing solutions ranged from 10.21 to 79.68%, 22.60 to 89.48%, 22.95 to 94-21% and 11.24–70.32% for the removal of deltamethrin, cypermethrin, chlorpyrifos, and endosulfan, respectively.