Feline leukemia virus (FeLV) was not detected in Florida pumas (Puma concolor coryi) in almost 20 yr of surveillance; however, the finding of two FeLV antigen-positive pumas during the 2002–2003 capture season led to an investigation of FeLV in the population. Between January 1990 and April 2007, the proportion of pumas testing FeLV antibody positive increased, with antibody-positive pumas concentrated in the northern portion of puma range. Five of 131 (4%) pumas sampled between July 2000 and April 2007 were viremic, with all cases clustered in Okaloacoochee Slough (OKS). Clinical signs and clinical pathology at capture were absent or included lymphadenopathy, moderate-to-severe anemia, and lymphopenia. All viremic pumas died; causes of death were septicemia (n=2), intraspecific aggression (n=2), and anemia/dehydration (n=1). Outcome after FeLV exposure in pumas was similar to that in domestic cats, with evidence of regressive, latent, and persistent infections. Management of the epizootic included vaccination, and as of April 2007, 52 free-ranging pumas had received one or more inoculations. Vaccinations were concentrated in OKS and in a band between OKS and the remainder of the puma population. There have been no new cases since July 2004; however, the potential for reintroduction of the virus remains.

The effect of heat stress (HS) and grain challenge (GC) on acid-base balance and rumen tissue histology in lambs was investigated using 24 yearling wether lambs (58 +/- 4.5 kg of BW) in a 2 x 2 factorial experiment with repeated measures for day (10, 14, and 17) of sampling. The factors were temperature [thermoneutral zone (TN) vs. HS] and diet (control vs. GC). Lambs were blocked by BW and assigned to 1 of 4 treatments in temperature-controlled rooms: 1) TN (temperature = 18 to 20 degrees C; relative humidity = 30%; 2) TN + GC; 3) HS (temperature = 35 degrees C for 9 h/d, 20 degrees C for 15 h/d; relative humidity = 40%); and 4) HS + GC. Venous blood samples were collected at 1800 on the first day of GC (d 10), in the middle of GC (d 14), and at the end of the trial (d 17) by jugular venipuncture and analyzed for pH, gases, hematocrit, plasma ions, and total protein. After all measurements in live animals were taken on d 17, lambs were slaughtered, and tissue samples were obtained from the ventral sac of the rumen for histological assessment. Except for the concentration of plasma glucose (P = 0.04) and total protein (P < 0.01), there were no (P > 0.05) diet x temperature interactions. With HS, the concentration of Na+ and Cl- in the control group decreased at d 14 and then increased by d 17, and respiration rates in the control group decreased linearly (P < 0.05). Compared with the control group, respiration rates and the concentration of Cl- in the GC lambs increased linearly over time, whereas the concentration of Na+ decreased linearly (P < 0.05) across time. Under HS, the partial pressure of carbon dioxide, total carbon dioxide, the partial pressure of oxygen and oxygen saturation, and the concentration of Mg2+, glucose, and HCO3- showed quadratic (P < 0.05) responses with time. In both treatments, DMI, base excess of extracellular fluid, base excess of blood, and standard bicarbonate increased linearly (P < 0.05), and hematocrit, plasma protein, Ca2+, anion gap, and plasma strong ion difference decreased linearly (P < 0.05) across day. Compared with the control group, the GC group had decreased papillae count in the ruminal ventral sac (1.3 vs. 1.5; P < 0.05). These results suggest that under HS the acidifying effects of GC on acid-base balance in lambs were counteracted in the short-term through respiratory adaptation.

Abstract. Chelonian intranuclear coccidiosis has been reported once, in two radiated tortoises (Geochelone radiata), and is apparently rare. We describe intranuclear coccidiosis diagnosed histologically in two radiated tortoises, three Travancore tortoises (Indotestudo forstenii), two leopard tortoises (Geochelone pardalis), one bowsprit tortoise (Chersina angulata), and one impressed tortoise (Manouria impressa). Infection was systemic and involved alimentary, urogenital, respiratory, lymphoid, endocrine, and integumentary systems. Trophozoites, meronts, merozoites, macrogametocytes, microgametocytes, and nonsporulated oocysts were seen histologically or by electron microscopy. Intracytoplasmic and extracellular stages of parasite development also were identified histologically. Sequencing of a coccidial 18S rRNA consensus polymerase chain reaction (PCR) product revealed a novel sequence that provided phylogenetic information and may be useful for further diagnostic test design. Intranuclear coccidiosis was associated with variable degrees of inflammation in all cases, was considered the cause of death in six tortoises, and was a substantial contributing factor to the cause of death in two tortoises.

Amphibian biology is intricate, and there are many inter-related factors that need to be understood before establishing successful Conservation Breeding Programs (CBPs). Nutritional needs of amphibians are highly integrated with disease and their husbandry needs, and the diversity of developmental stages, natural habitats, and feeding strategies result in many different recommendations for proper care and feeding. This review identifies several areas where there is substantial room for improvement in maintaining healthy ex situ amphibian populations specifically in the areas of obtaining and utilizing natural history data for both amphibians and their dietary items, achieving more appropriate environmental parameters, understanding stress and hormone production, and promoting better physical and population health. Using a scientific or research framework to answer questions about disease, nutrition, husbandry, genetics, and endocrinology of ex situ amphibians will improve specialists’ understanding of the needs of these species. In general, there is a lack of baseline data and comparative information for most basic aspects of amphibian biology as well as standardized laboratory approaches. Instituting a formalized research approach in multiple scientific disciplines will be beneficial not only to the management of current ex situ populations, but also in moving forward with future conservation and reintroduction projects. This overview of gaps in knowledge concerning ex situ amphibian care should serve as a foundation for much needed future research in these areas.

Combinations of rapid, simple HIV antibody assays provide sensitivity and specificity performance comparable to EIA/Western blot. Application of these combinations in the WHO alternative testing strategies provides an inexpensive and effective method of determining HIV status. Assay combinations using these strategies can be easily performed in small, rural laboratories and have been implemented in routine HIV screening in Honduras.