No abstract

Brucellosis in Palestine is a problem of huge concern due to the human health impact, as consequence of the high prevalence level of the infection in livestock. The purpose of the research was to give an insight of the current epidemiological peculiarities of the brucellosis in livestock versus human population. This paper processed all data of the brucellosis’ control program and the epidemio-surveillance results. The seroprevalence of brucellosis in 1999, was 18% in sheep and goat and 80% in flocks, and more than 800 human cases. As result of mass vaccination, with 80% coverage vaccination rate, the decrease of brucellosis was registered: 4.8% in animals and 40% in flock; human infection less than 200 cases. The main conclusion of our study is that the vaccination must be in force up to the level of prevalence, below 2% in animals, allowing the next step, the “test-and-slaughter” strategy, toward the eradication goal.

Brucellosis is endemic in Palestine and therefore since 1998 a national program of brucellosis control has been launched. In the traditional breeding area of small ruminants, brucellosis is often reported in humans, mainly as a food borne disease. Any control strategy requires a well-functioning surveillance system, the co-operation with owners and a sustainable financial support. The aim of this study is sizing up of the current situation of knowledge, of the attitude and practice (KAP) regarding brucellosis, among sheep and goat farmers. A cross-sectional survey was conducted in 118 participants through an anonymous questionnaire to assess to which extent the flock owners are familiar with the knowledge and practices related to brucellosis of small ruminants. The designs of the questionnaire consider the implementation of KAP. Results shown that farmers, independent on their education, heard about brucellosis (100%) and all of them consider the washing of hands as necessary after close contact with animals or their products, and animal vaccination as being necessary to prevent brucellosis. Despite the educational level, almost all owners are aware about the risk of exposure of their family to animals infected with Brucella: they are considering the heating of cheese at boiling point and the use of gloves, as appropriate practices to reduce the exposure to Brucella. Great majorities of the participants (89.8%) have good knowledge about the transmission of Brucella from animals to humans, but only 37.3% correctly answered about the transmission of brucellosis by drinking raw milk, eating unpasteurized cheese, eating raw meat, liver, spleen and kidney or by contact with an aborted foetus and placenta fluid. Referring to the brucellosis' prevention, 83% answered that boiling of milk and cheese and wearing of gloves when handling an aborted foetus could prevent it: these answers fit with the level of knowledge. From the questioned owners, 28.8% knew that boiling the milk affect his nutritive value; 84.7% think that owners of animals and their families are more exposed to brucellosis and 11.9% of their families have been diseased. Relative good general knowledge of owners about brucellosis was recorded, but high-risk behaviors still exist. Awareness campaigns on the control and prevention of the brucellosis are success stories, and government agencies should continue the public education.UDC Classification: 614.9; DOI: http://dx

This paper presents synthesis, characterization and cytotoxicity assessment of six S-alkylated 1,2,4-triazoles derivatives that were obtained by treatment of some 1,2,4-triazole-3-thiones containing in the molecule phenylsulfonylphenyl and propyl or 4-bromophenylsulfonylphenyl and 4-methoxyphenyl fragments with different alkylation agents (ethyl bromide, ethyl chloroacetate or phenacyl bromide) in basic media. The synthesized compounds were physico-chemically characterized and the IR, 1H-NMR, 13C-NMR spectra were consistent with the assigned structures. The cytotoxicity of S-alkylated derivatives was evaluated on Daphnia magna crustacean.

This paper presents the synthesis of new heterocyclic compounds from oxazoles and triazinones classes. Some 2-(4-(4-X-phenylsulfonyl)benzamido)acetic acids were condensed with 4-iodobenzaldehyde in presence of acetic anhydride and sodium acetate, when the new corresponding unsaturated oxazol-5(4H)-one were obtained. Through the reaction of these oxazol-5(4H)-ones with phenylhydrazine, in presence of the acetic acid and sodium acetate, the new heterocyclic compounds from 1,2,4-triazin-6(5H)-ones class were obtained. The structures of synthesized compounds have been confirmed by spectral methods (FT-IR, 1H-NMR, 13C-NMR and MS) and elemental analysis. The cytotoxicity of the compounds was evaluated using the Daphnia magna and Artemia salina bioassays.