Carbohydrate tetrachloride induces injury of liver parenchyma evoking fast and severe liver fibrosis, and is associated with irreversible structural and functional changes in testes, reducing fertility, decreasing potential pregnancy rate, and affecting its development. Liver showed high potential to regenerate, however the self-restoring after liver fibrosis was not accompanied with recovery of the reproductive system.

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Mesenchymal stem cells (MSCs) are ideal candidates for cell-based therapy aimed at tissue repair and immunomodulation. Aim. To study the survival of transplanted human MSCs from umbilical cord Wharton's Jelly (hWJ-MSCs) in the animal model of experimental osteoarthritis (OA) in rats after injecting cells into a knee joint and to explore the effect of collagen scaffold on the cell survival in vivo. Methods. MSC isolation and cultivation in vitro. Immunological phenotyping of propagated hWJ-MSCs was performed by fl ow cytometry. The retention of transplanted cells was studied by the PCR revealing of human specifi c sequences in genomic DNA extracted from animal tissues. Results. hWJ-MSCs, both individual and grown on scaffold, were used and it was shown by PCR that human alpha-satellite DNA was detected on the fi rst day in the immunocompetent OA animals inside the injured knee joint. In the collagen matrix (in the model of subcutaneous implantation) human alpha-satellite DNA was detected on the 5 th day but was not detected on the 12 th day. Conclusions. According to the PCR results, hWJ-MSCs survived in the OA animal model for a short period. Collagenic scaffold increased the residence time of donor cells in the recipients. hWJ-MSCs may be considered as a perspective cell source for the treatment of OA in human.

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