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Our previous study suggested that green light promotes broiler growth during the early stage [posthatching day (P) 0 to P26], and blue light enhances growth during the later stage (P27 to P49). The purpose of this study was to improve broiler growth and productive performance by using a combination of monochromatic lights at critical points between the early and later stages of growth. A total of 512 male Arbor Acres broilers on P0 were reared under white light (W), red light (R), green light (G), and blue light (B) by using light-emitting diode lamps at 15 ± 0.2 lx from P0 to P26 (16 replicate pens/group, 8 birds/pen), and then switching to another color of light until P49 (4 replicate pens/group, 8 birds/pen). As compared with single monochromatic lights, broilers reared in environments under combinations of monochromatic lights, W→G, R→B, G→B, and B→G, attained heavier BW than those reared in environments under W→W (3.18 to 12.00%), R→R (1.96 to 18.14%), G→G (0.85 to 5.08%), and B→B (0.39 to 4.70%), respectively. In addition, feed conversion ratios in the W→B, R→B, and G→B combinations were lower than feed conversion ratios for W→W (15.86%, P < 0.05), R→R (18.41%, P < 0.05), and G→G (3.37%), respectively. Moreover, the eviscerated carcass weight and breast, thigh, and crus muscle weights under G→B were greater by 0.40 to 56.23% than were those for the other light groups except W→B (eviscerated carcass) and B→G (breast muscle). The results suggest that the application of the G→B and B→G exchanges can be used successfully to improve growth and productive performance in broilers.

Natural vitamin E was extracted by supercritical fluid extraction of carbon dioxide (SFE-CO 2 ) from wheat germ. Several SFE-CO 2 parameters, such as extracting pressure, extracting temperature, and flow rate of carbon dioxide were examined as the independent variables of central composite rotate design (CCRD). Through the response surface methodology (RSM), the optimal processing conditions were determined and the quadratic response surfaces were drawn from the mathematical models. The results demonstrated that the extracting pressure, temperature, pressure ϫ ϫ ϫ ϫ ϫ temperature interaction, and flow rate of CO 2 significantly affected the yield of the natural Vitamin E's extraction, while two interactions containing the flow rate of CO 2 had no significant effect on the yield of natural vitamin E. The optimal processing conditions of the extraction of natural vitamin E in wheat germ by SFE-CO 2 were: extracting pressure 5000 PSI, extracting temperature 316 K, and flow rate of carbon dioxide 1.7 ml/min. Optimum value predicted by RSM for the concentration of natural vitamin E was 2307 mg/100g. Close agreement between experimental and predicted values was obtained.

Two experiments were conducted to evaluate the effect of supplementing a corn-soybean meal-based diet with an enzyme complex containing amylase, protease and xylanase on the performance, intestinal health, apparent ileal digestibility of amino acids and nutrient digestibility of weaned pigs. In Exp. 1, 108 piglets weaned at 28 d of age were fed one of three diets containing 0 (control), 100, or 150 ppm enzyme complex for 4 wks, based on a two-phase feeding program namely 1 to 7 d (phase 1) and 8 to 28 d (phase 2). At the end of the experiment, six pigs from the control group and the group supplemented with 150 ppm enzyme complex were chosen to collect digesta samples from intestine to measure viscosity and pH in the stomach, ileum, and cecum, as well as volatile fatty acid concentrations and composition of the microflora in the cecum and colon. There were linear increases (p<0.01) in weight gain, gain: feed ratio and digestibility of gross energy with the increasing dose rate of enzyme supplementation during the whole experiment. Supplementation with enzyme complex increased the digesta viscosity in the stomach (p<0.05) and significantly increased (p<0.01) the concentrations of acetic, propionic and butyric acid in the cecum and colon. Enzyme supplementation also significantly increased the population of Lactobacilli (p<0.01) in the cecum and decreased the population of E. coli (p<0.05) in the colon. In Exp. 2, six crossbred barrows (initial body weight: 18.26±1.21 kg), fitted with a simple T-cannula at the distal ileum, were assigned to three dietary treatments according to a replicated 3×3 Latin Square design. The experimental diets were the same as the diets used in phase 2 in Exp. 1. Apparent ileal digestibility of isoleucine (p<0.01), valine (p<0.05) and aspartic acid (p<0.05) linearly increased with the increasing dose rate of enzyme supplementation. In conclusion, supplementation of the diet with an enzyme complex containing amylase, protease and xylanase improved piglet performance. This is likely a result of improvement in nutrient digestibility, volatile fatty acid concentrations and bacteria ratio in the large intestine.