Eucalyptus spp., most of which are native to Australia, are one of the world's most commonly planted forestry crops, and have been widely planted in the tropics and subtropics. Eucalyptus trees are distributed in more than 600 counties in 19 provinces/regions throughout China, especially in the southern regions of the country (1). From April of 2013 to May of 2014, eucalyptus trees were noted to be wilting and dying rapidly in Kunming, Yunnan Province (25°02′ N, 102°42′ E), southwest China. Symptomatic trees typically have many wilted or dead leaves hanging throughout the tree or on some major branches, sometimes followed by death of the whole tree. Dark brown to black discoloration can be seen in the woody xylem, and elongated cankers were also present on some affected trees. A fungus was consistently isolated from the leaves and cankers when symptomatic tissue was incubated between two slices of fresh carrot root. Spore masses were removed from the apices of perithecia, transferred to malt extract agar medium (MEA), and incubated at 25°C. Perithecia developed on the medium, and were black, globose, 212.5 to 242.4 × 207.5 to 254.2 μm, and possessed a long black neck (952.3 to 1,303.3 μm). Ascospores had the typical “hat” morphology and were 4.3 to 5.2 × 3.0 to 3.8 μm. Cylindrical endoconidia (11.2 to 22.2 × 3.9 to 6.1 μm) were found. Chlamydospores produced on media were ovoid and smooth, and were 7.8 to 9.7 × 9.9 to 12.8 μm. Chains of barrel-shaped conidia were not found. PCR amplification and sequencing of the ITS region of rDNA were carried out for one isolate, E2-2, using the procedures of Thorpe et al. (3). Analysis of ITS sequence data (GenBank Accession No. KJ511481) showed that the isolates were 99% homologous to the isolate of C. fimbriata from diseased Colocasia esculenta in Cuba, China, and Hawaii (AY526304 to 06) by BLAST analysis. Thus, the fungus was identified as C. fimbriata based on morphological and molecular characteristics. Pathogenicity tests were made on 1-year-old E. grandis seedlings as follows. A conidial suspension of each isolate was diluted to 106 conidia/ml, and 0.2 ml was injected into wounds on three petioles on each of five healthy plants of E. grandis, and control seedlings were injected with sterile water that had been placed on MEA plates. The seedlings were incubated at 25°C in moist chambers. After 3 days, all inoculated E. grandis plants showed dark brown to black discoloration in the leaf axils. After 5 days, leaf wilting was present. C. fimbriata was re-isolated from all inoculated symptomatic tissue. No symptoms were visible in the control plants and no fungus was isolated from them. Ceratocystis wilt was first observed in eucalyptus in 1997 in the state of Bahia. This was followed by a report of C. fimbriata causing wilt of E. grandis in the Republic of Congo, Uganda, and Uruguay (2). Chen et al. reported two species of Ceratocystis, C. acaciivora and a previously undescribed species C. chinaeucensis, from eucalyptus plantations in Guangdong Province in China (1). To our knowledge, this is the first report of C. fimbriata causing wilt of eucalyptus in China. References: (1) S. F. Chen et al. Fungal Diversity 58:267, 2011. (2) F. A. Ferreira et al. Fitopatol. Bras. 24:284, 1999. (3) D. J. Thorpe et al. Phytopathology 95:316, 2005.

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The loquat (Eriobotrya japonica (Thunb.) Lindl.), an important perennial evergreen fruit crop that originated in China and is also cultivated as an ornamental plant, is well known all over the world for high nutritional, medicinal, economic, and ecological values (4). So far, it has been grown in more than 30 countries (1) and is becoming an important industry in China, Spain, Japan, India, Pakistan, and Turkey (2). During an investigation from May to August of 2013, severely withered loquat plants were observed in Kunming city of Yunnan Province (25°02′ N; 102°42′ E), Southwest China. Initial symptoms were brown lesions of leaves and canker on one to several branches, leaves of the whole tree turned wilted. Cross sections of diseased plants revealed irregularly shaped brown discoloration in the xylem of the trunk. A fungus was consistently isolated from the leaf when diseased leaves were incubated between two slices of fresh carrot root. Spore masses were picked from the apices of perithecia and transferred to malt extract agar medium (MEA) and incubated at 25°C. After perithecium formation, observed perithecia were black, globose (176.0 to 303.2 × 186.0 to 274.3 μm) and showed a long black neck (634.2 to 809.9 μm). Ascospore had the typical format of a “hat” with dimensions of 4.6 to 6.3 × 3.3 to 4.8 μm. Cylindrical endoconidia (7.1 to 36.1 × 2.9 to 6.0 μm) were found. Chlamydospores were ovoid or obpyriform and smooth (8.6 to 12.1 × 6.9 to 12.1 μm). PCR amplification was carried out for one isolate, YT2. The ITS region of rDNA was sequenced using the procedures of Thorpe et al (3). Analysis of ITS sequence data (GenBank Accession Nos. KF963101 and KF963102) showed that the isolates were 99% homologous to the isolate of Ceratocystis fimbriata from diseased Colocasia esculenta in Cuba, China, and Hawaii (AY526304 to 06) by BLAST analysis. Therefore, the fungus was identified as C. fimbriata based on morphological and molecular characteristics. Pathogenicity of the six isolates from this study was tested by inoculation of 1-year-old pot grown seedlings of loquat. The soil of six plants was inoculated by drenching with 40 ml spore suspension (106 spores/ml). Control plants were inoculated with 40 ml of sterile distilled water. The plants were maintained in a controlled greenhouse at 25°C and watered weekly. After inoculation for 1 week, all plants produced wilt symptoms; as the disease progressed, leaves withered and died after 3 weeks of inoculation while control plants remained symptomless. C. fimbriata was successfully re-isolated from the infected trees and no fungal growth was observed in the controls. The pathogenicity assay showed that C. fimbriata was pathogenic to loquat. To our knowledge, this is the first report of C. fimbriata causing wilt of loquat in China. Since C. fimbriata is one of the most aggressive plant pathogens on a wide variety of perennial as well as agronomic crop plants worldwide, and the infection can pose a significant threat to the production of loquat, it is critical to deploy appropriate management strategies to limit the fungus spread. References: (1) J. J. Feng et al. Acta Hort. 750:117, 2007. (2) J. Janick. Acta Hort. 750:27, 2007. (3) D. J. Thorpe et al. Phytopathology 95:316, 2005. (4) J. Yan et al. Pak. J. Bot. 44:1215, 2012.