For evaluation of the viability of partial liver graft from a living donor, we investigated energy production of mitochondria and radical scavenging enzyme activities in partial and whole liver transplantation in pigs. The values of adenosine triphosphate (ATP) and total adenine nucleotide (TAN) of the partial liver graft were higher than those of the whole liver graft, whereas the hypoxanthine of the partial liver graft was lower than that of the whole liver graft. There was no statistical difference in the radical scavenging enzyme activities between the two groups. The values of respiratory control ratio (RCR) in both groups were above 3.0 and there was no statistical difference. The survival rates of pigs received partial liver and whole liver graft with 2 to 3 hr cold preservation was 71% and 91%, respectively and there was no statistical difference between two groups. These results suggest that viabilities of the partial liver graft from the living donor are satisfactory enough, compared with those of whole liver graft from a cadaver, living-donor liver transplantation; graft viability; pig; partial graft
In clinical liver transplantation, a venous bypass that rechannels the blood flow from the inferior vena cava and portal vein to the superior vena cava has been used to maintain the venous return. However, the usage of mechanical shunt has given rise to derangements of blood coagulability and fibrinolysis. Therefore, changes of coagulability and fibrinolysis during the venous bypass were examined in dogs using a centrifugal pump (Bio-Pump®), and the effect of gabexate mesilate for coagulation were studied. Venous bypass from the inferior vena cava and the portal vein to the external jugular vein was performed in mongrel dogs (group A : bypass without pump, group B : bypass with the Bio-Pump, group C : bypass with the Bio-Pump and the addition of gabexate mesilate). In group A, blood pressure gradually decreased, but in groups B and C it maintained the pre-bypass level. All results of coagulation parameters and fibrinolytic measurements of group B were not significantly different from those of group A ; only partial thromboplastin time was prolonged in group B. However, this prolongation was effectively prevented by the addition of gabexate mesilate in group C.veno-venous bypass ; liver transplantation ; gabexate mesilate ; fibrinolysis ; coagulation testIn clinical liver transplantation, obstruction of the inferior vena cava and the portal vein causes hypotension and a decrease in cardiac output when hepatectomy or an implantation of a new liver is carried out (Pappas et al. 1971). Recently, a pump-driven veno-venous bypass that transmits blood from the inferior vena cava and the portal vein into the superior vena cava is employed to maintain the cardiac output and arterial pressure during anhepatic phase (Shaw et al. 1984). The systemic heparinization using veno-venous bypass is discouraged because of major difficulties in reversing the heparin effect in patients with
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