The High Resolution Fly's Eye (HiRes) experiment has observed the Greisen-Zatsepin-Kuzmin suppression (called the GZK cutoff) with a statistical significance of five standard deviations. HiRes' measurement of the flux of ultrahigh energy cosmic rays shows a sharp suppression at an energy of 6 x 10(19) eV, consistent with the expected cutoff energy. We observe the ankle of the cosmic-ray energy spectrum as well, at an energy of 4 x 10(18) eV. We describe the experiment, data collection, and analysis and estimate the systematic uncertainties. The results are presented and the calculation of the statistical significance of our observation is described.

Autophagy is a lysosome-based degradation pathway. During autophagy, lysosomes fuse with autophagosomes to form autolysosomes. Following starvation-induced autophagy, nascent lysosomes are formed from autolysosomal membranes through an evolutionarily conserved cellular process, autophagic lysosome reformation (ALR), which is critical for maintaining lysosome homeostasis. Here we report that clathrin and phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P(2)) regulate ALR. Combining a screen of candidates identified through proteomic analysis of purified ALR tubules, and large-scale RNAi knockdown, we unveiled a tightly regulated molecular pathway that controls lysosome homeostasis, in which clathrin and PtdIns(4,5)P(2) are the central components. Our functional study demonstrates the central role of clathrin and its associated proteins in cargo sorting, phospholipid conversion, initiation of autolysosome tubulation, and proto-lysosome budding during ALR. Our data not only uncover a molecular pathway by which lysosome homeostasis is maintained through the ALR process, but also reveal unexpected functions of clathrin and PtdIns(4,5)P(2) in lysosome homeostasis.

The composition of ultra-high-energy cosmic rays is measured with the High Resolution Fly's Eye cosmic-ray observatory data using the X max technique. Data were collected in stereo between 1999 November and 2001 September. The data are reconstructed with well-determined geometry. Measurements of the atmospheric transmission are incorporated in the reconstruction. The detector resolution is found to be 30 g cm À2 in X max and 13% in energy. The X max elongation rate between 10 18.0 and 10 19.4 eV is measured to be 54:5 AE 6:5 stat ð Þ AE 4:5 sys ð Þg cm À2 per decade. This is compared with predictions using the QGSJet01 and SIBYLL 2.1 hadronic interaction models for both protons and iron nuclei. CORSIKA-generated extensive air showers are incorporated directly into a detailed detector Monte Carlo program. The elongation rate and the X max distribution widths are consistent with a constant or slowly changing and predominantly light composition. A simple model containing only protons and iron nuclei is compared with QGSJet and SIBYLL. The best agreement between the model and the data is for 80% protons for QGSJet and 60% protons for SIBYLL. Subject headingg s: acceleration of particles -cosmic rays -large-scale structure of universe