Chrysophyllum perpulchrum (Sapotaceae) is used in the traditional Ivory Coast pharmacopeia to cure fevers. The extract of C. perpulchrum used for this study was the powdered form obtained from the maceration of the dried plant bark in 96% methanol, followed by evaporation to dryness. In the present study, the antioxidative and radical-scavenging activities of the methanolic extract were studied with three standard biological tests: DPPH reduction, ferric thiocyanate (FTC) lipidic peroxidation inhibition and thiobarbituric acid reacting substances (TBARS). Gallic acid and quercetin were used as references. The total amount of phenolic compounds in the extract was determined by ultraviolet (UV) spectrometry and calculated as gallic acid equivalents. Catechin and two dimeric procyanidins were found to be the compounds responsible for the activities. They were chemically dereplicated in the extract by LC-MS. For quantitation purposes, they were isolated by successive chromatographic methods and characterized by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectrometry. The quantities of these compounds in C. perpulchrum were 5.4% for catechin (P1), and 5.6 and 9.2% for dimers (compounds 2 (P2) and 3 (P3)), respectively. They displayed antioxidant activity with IC50 values of 2.50 ± 0.15 µg/mL (P1), 2.10 ± 0.2 µg/mL (P2) and 2.10 ± 0.1 µg/mL (P3). The total extract, the active fractions and the pure compounds inhibited the lipid peroxidation by the FTC method and the TBARS method in the range of 60%. These values were comparable to those seen for quercetin.

An ethnobotanical study with phytochemical screening of some Pteridophytes was carried out in the classified forest of Yapo-Abbé in the south of Côte d'Ivoire, in the Agboville Department, precisely in the subprefecture of Azaguié. The objective of this study was to show the different uses of the Pteridophytes of this forest. The approach method is an ethnobotanical survey carried out among the populations of the study area. This survey consisted in drafting a questionnaire beforehand to apprehend the multiple uses of Pteridophytes among the surrounding population and to develop a photo album of Pteridophytes to be presented during the interview. The data collection was carried out using the semi-structured interview method and with the help of an interpreter guide. It identified 19 species of Pteridophytes including six (6) species used as medicinal plants. No other use of these taxa was mentioned. The whole plant is the most solicited or 73.33% for the preparation of medicinal recipes. Various methods of preparation such as decoction (33.3%), kneading (33.3%), softening (20%) and use in the fresh state (13.3%) are used for recipes. single species. There are also different modes of administration of phytomedicaments: drink (42.85%), local application (37.71%), mastication (7.14%) and massage (7.14%). The phytochemical sorting performed on the hydroethanolic extracts revealed that the different drugs of these plants contain sterols, polyterpenes, polyphenols, European Scientific Journal November 2018 edition Vol.14, No.33 ISSN: 1857 – 7881 (Print) e - ISSN 1857- 7431 174 flavonoids, catechin tannins, alkaloids and quinones which give them various therapeutic properties.

An ethnobotanical survey was carried out in the District of Yamoussoukro (Central Côte d’Ivoire) in order to evaluate the biocontrol potential against fungal plant pathogens, of Euphorbiaceous plants used as medicines by the native population. Twelve (12) species were collected. The antifungal activity was assessed using the two-fold dilution method on PDA medium with ethanolic and aqueous extract derived from leaves of six plants. Results pointed out that only 70% ethanolic extracts from Alchornea cordifolia (EE13) possess fungicidal properties on both Fusarium sp. and Phytophthora sp. The antifungal activities are more effective with ethanolic extracts than aqueous extracts. With a minimal inhibitory concentration MIC = 25±0.0 mg/ml, a minimal concentration for 50% of inhibition IC50 = 10±0.05 mg/ml and a minimal fungicide concentration MFC = 200±0.0 mg/ml Fusarium sp. appears to be the most sensitive. Phytophthora sp. is less sensitive with a MIC = 50±0.0 mg/ml, an IC50 = 12±0.05 mg/ml and a MFC = 200±0.0 mg/ml. Phytochemical screening revealed the presence of seven major groups of compounds among which saponins and catechin are the most abundant in aqueous extracts. Polyphenols, flavonoids, polyterpenes and sterols as well as tannins are present in both alcoholic and aqueous extracts of Alchornea cordifolia. These compounds could justify its antifungal activity and traditional use.