This study aimed to examine the changes in performance and emission values in a four-stroke and three-cylinder diesel engine by using pure diesel fuel and fuels created by adding TiO2 nanoparticles. Adding TiO2 nanoparticles to the fuel aimed to improve the combustion characteristics of the diesel engine and reduce the harmful effects of exhaust emissions. Pure diesel and TiO2 nanoparticle added fuel samples in three distinct amounts, 25 ppm, 50 ppm, and 75 ppm, were prepared in magnetic and ultrasonic mixers as D100, D100+25TiO2, D100+50TiO2, and D100+75TiO2. All fuel samples used in the research were tested at a constant speed of 1800 revolutions per minute (rpm) at 25%, 50%, 75%, and 100% full loads. When TiO2-added fuels were compared to pure diesel, there was a 15.12% rise in brake thermal efficiency at %25 load and a 13.36% drop in brake specification fuel consumption at %25 load. EGT values also increased with the increase in load and adding TİO2. The amount of CO2 in exhaust emissions increased by 5% at maximum load in the fuel with the highest TiO2 additive according to neat diesel. There was an average increase of 11.44% in NOX emissions for all loads with TiO2 addition. The results show that the fuel mixture created by adding TiO2 nanoparticles can be used in certain proportions in diesel engines and that the TiO2 addition positively improves the combustion properties, engine performance, and exhaust gas emissions.