Untitled

Abstract

Transglutaminase (TGase) activities were measured in rat tissues 1-7 days after intraperitoneal injection of saline or lipopolysaccharide (LPS) and in the cells and media from pre-confluent human fibroblasts cultured for two days in the presence or absence of LPS. epsilon (gamma-Glutamyl)lysine and [3H]putrescine-labelled gamma-glutamyl derivatives in extracellular and cellular fibroblast proteins were also measured. Three effects of LPS were observed. Firstly, total TGase activity is greater in the tissues from the LPS-injected animals, with the maximum increase occurring at 1 day in dermis, epidermis and liver, at 5 days in the aorta and, after a decrease at 2-5 days, at 7 days in the panniculus muscle. Secondly, the fraction of the total activity which is buffer-extractable is greater on days 1 and/or 2 in all the tissues from the LPS-injected rats. Thirdly, in cultures of human fibroblasts, LPS increases that fraction of bound [3H]putrescine and of TGase and its gamma-glutamylamine products which occurs in the extracellular medium. In addition, a higher concentration of TGase-derived crosslinks was found in extracellular as opposed to intracellular proteins. In conjunction with previous findings in skin wound healing and in atherosclerosis these results support the concept of an extracellular function for tissue TGase and indicate that there is a widespread association of increases in TGase and its extracellular products with inflammation and the healing or fibrotic processes which follow it.

Transglutaminase 2 Induces Nuclear Factor-κB Activation via a Novel Pathway in BV-2 Microglia

“…7B) (*, p ϭ 0.017; **, p ϭ 0.04, n ϭ 6). DISCUSSION We and others have observed that TGase 2 is induced after LPS treatment in various tissues and cells (9,30,49). In this study, we showed that an increase of TGase activity is associated with NF-B activation via an IKK-independent pathway in microglia and SH-SY5Y cells.…”

The proinflammatory protein HMGB1 is a substrate of transglutaminase-2 and forms high-molecular weight complexes with autoantigens

“…2, B, lanes 5-12, and C). Consistent with a previous report (23), TG2 levels in the liver were increased 24 h after LPS administration (Fig. 2, B and D).…”

A Novel Therapeutic Target in Inflammatory Uveitis: Transglutaminase 2 Inhibitor

“…Increased expression of TGase 2 may exacerbate inflammation by amplifying NF-kB activation through I-kBa depletion. Furthermore, TGase 2 inhibition reduced nitric oxide generation during LPS-induced inflammation [13, 18]. …”