Untitled

Abstract

Reactive oxygen species, released by phagocytes, are involved in tissue injury in inflammatory bowel diseases. The aim of our study was to evaluate peripheral neutrophil function in patients with ulcerative colitis (N = 66) and Crohn's disease (N = 62) with respect to disease activity and extent, using chemiluminometry after three stimuli. Twenty-seven healthy subjects were enrolled as controls. Neutrophils from ulcerative colitis and Crohn's disease patients had a significantly higher response than those from controls following phorbol myristate acetate (86.6 +/- 6.5, 173.8 +/- 11.9, 167.5 +/- 12.2 mV, P < 0.0001), formyl-methionyl-leucyl-phenylalanine (39.5 +/- 3.4, 41.3 +/- 2.7, 58.6 +/- 4.7 mV, P < 0.001), and zymosan (142.6 +/- 10.4, 223.7 +/- 8.9, 231.2 +/- 9.5 mV, P < 0.0001) administration. The increased response was observed during both active disease and remission. The highest chemiluminescence values were found in patients with active ulcerative pancolitis and ileal Crohn's disease. The activation of circulating neutrophils may indicate persistent intestinal inflammation or may be triggered by luminal factors even in the absence of symptoms.

Punicic Acid a Conjugated Linolenic Acid Inhibits TNFα-Induced Neutrophil Hyperactivation and Protects from Experimental Colon Inflammation in Rats

“…TNFα is involved in priming of neutrophil ROS production which is a key process in inflammatory reaction (17). The presence of primed neutrophils was described in various inflammatory diseases such as rheumatoid arthritis, IBD, coronary heart disease and diabetes [18], [49], [50]. One of the molecular mechanism underlying this priming state of neutrophil-ROS production is the phosphorylation of p47phox on Ser345.…”

Adsorptive Granulocyte and Monocyte Apheresis versus Prednisolone in Patients with Corticosteroid-Dependent Moderately Severe Ulcerative Colitis

“…The condition is associated with elevated peripheral blood granulocytes and monocytes/macrophages [1, 2, 3, 4, 5]that show activation behavior and increased survival time [6, 7, 8, 9, 10]. Another pathological feature of active UC is increased generation and activities of inflammatory cytokines which can initiate and perpetuate the disease [11, 12, 13, 14, 15].…”

Neutrophil Priming as a Surgery-Related Risk Factor for Postoperative Infectious Complications in Patients with Ulcerative Colitis

“…Furthermore, increases in tissue myeloperoxidase activity and NE activity were evident and well correlated with the area of colonic ulcers after induction of experimental colitis [24]. In the present study, the patients with a preoperative high NE level had a longer disease duration, suggesting that neutrophil activity, probably triggered by the network of cytokines produced in the inflamed gut, may be modulated not only at the disease site but also in the bloodstream [22]. …”

“…NE is stored in primed neutrophils and its circulating level reflects neutrophil activation. In UC patients, circulating neutrophils are primed not only in active disease but also in clinical remission [22]. Various findings have been reported concerning the relationship between UC and NE.…”

“…It was shown that chemiluminescence was significantly higher in the peripheral neutrophils of UC patients than in those of control subjects. Moreover, the maximal response time was significantly shorter in patients than in control subjects, thus demonstrating the activation phase of these cells [22]. NE largely contributes to the proteolysis of phagocytosed proteins.…”