05 gigapixel microscopy using a flatbed scanner

Zheng, Guoan; Ou, Xiaoze; Yang, Changhuei

doi:10.1364/boe.5.000001

Cited by 33 publications

(27 citation statements)

References 23 publications

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“…Recently, CIS has been explored as a low cost solution for wide FOV biomedical imaging [8,9]. Zheng, et al built a wide FOV brightfield scanning microscopy system based on a closed-circuit television (CCTV) lens for image magnification and a flatbed scanner for image acquisition [8].…”

Section: Introductionmentioning

confidence: 99%

A wide field-of-view scanning endoscope for whole anal canal imaging

Han¹,

Huangfu²,

Lai³

et al. 2015

Biomed. Opt. Express

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Abstract:We report a novel wide field-of-view (FOV) scanning endoscope, the AnCam, which is based on contact image sensor (CIS) technology used in commercialized business card scanners. The AnCam can capture the whole image of the anal canal within 10 seconds with a resolution of 89 μm, a maximum FOV of 100 mm × 120 mm, and a depthof-field (DOF) of 0.65 mm at 5.9 line pairs per mm (lp/mm). We demonstrate the performance of the AnCam by imaging the entire anal canal of pigs and tracking the dynamics of acetowhite testing. We believe the AnCam can potentially be a simple and convenient solution for screening of the anal canal for dysplasia and for surveillance in patients following treatment for anal cancer.

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Section: Introductionmentioning

confidence: 99%

A wide field-of-view scanning endoscope for whole anal canal imaging

Han¹,

Huangfu²,

Lai³

et al. 2015

Biomed. Opt. Express

Self Cite

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“…For a large FOV microscope system, the aberration and, by extension, pupil function can be expected to show spatial variations [3,24]. To ensure the effectiveness of the EPRY-FPM algorithm, we segment the entire FOV into small tiles where in each tile the aberration can be considered as constant [1,3].…”

Section: Epry-fpm For Large Fov High Resolution Image Reconstructionmentioning

confidence: 99%

Embedded pupil function recovery for Fourier ptychographic microscopy

Ou¹,

Zheng²,

Yang³

2014

Opt. Express

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365

256

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Abstract:We develop and test a pupil function determination algorithm, termed embedded pupil function recovery (EPRY), which can be incorporated into the Fourier ptychographic microscopy (FPM) algorithm and recover both the Fourier spectrum of sample and the pupil function of imaging system simultaneously. This EPRY-FPM algorithm eliminates the requirement of the previous FPM algorithm for a priori knowledge of the aberration in the imaging system to reconstruct a high quality image. We experimentally demonstrate the effectiveness of this algorithm by reconstructing high resolution, large field-of-view images of biological samples. We also illustrate that the pupil function we retrieve can be used to study the spatially varying aberration of a large field-of-view imaging system. We believe that this algorithm adds more flexibility to FPM and can be a powerful tool for the characterization of an imaging system's aberration.

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“…By introducing these pupil functions in the recover process, the reported scheme may be able to compensate for these aberrations. Gigapixel microscope can be built using high-capacity photographic lenses [37]. 4) The reported scheme is capable of extending the FP concept to macroscopic imaging settings, where objects are placed in the far field.…”

Section: Resultsmentioning

confidence: 99%

“…We also note that, in modern optical system design, there is a gap between the information capacity (i.e., the space-bandwidth product, SBP [34]) of an optical system and that of a digital recording device [35,36]. For example, a simple closed-circuit-television (CCTV) lens can provide a field-of-view of 75 mm 2 and a diffraction limited resolution of 0.78 µm (characterized at 632 nm wavelength), leading to a SBP of 0.5 billion [37]. To fully sample the field transferred by this lens to its image plane, we need at least 0.5 billion effective pixels on the image sensor, which is orders of magnitude higher than the pixel count of existing CCD/CMOS image sensors.…”

Section: Ementioning

confidence: 99%

Aperture-scanning Fourier ptychography for 3D refocusing and super-resolution macroscopic imaging

et al. 2014

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Abstract:We report an imaging scheme, termed aperture-scanning Fourier ptychography, for 3D refocusing and super-resolution macroscopic imaging. The reported scheme scans an aperture at the Fourier plane of an optical system and acquires the corresponding intensity images of the object. The acquired images are then synthesized in the frequency domain to recover a high-resolution complex sample wavefront; no phase information is needed in the recovery process. We demonstrate two applications of the reported scheme. In the first example, we use an aperture-scanning Fourier ptychography platform to recover the complex hologram of extended objects. The recovered hologram is then digitally propagated into different planes along the optical axis to examine the 3D structure of the object. We also demonstrate a reconstruction resolution better than the detector pixel limit (i.e., pixel super-resolution). In the second example, we develop a camera-scanning Fourier ptychography platform for super-resolution macroscopic imaging. By simply scanning the camera over different positions, we bypass the diffraction limit of the photographic lens and recover a super-resolution image of an object placed at the far field. This platform's maximum achievable resolution is ultimately determined by the camera's traveling range, not the aperture size of the lens. The FP scheme reported in this work may find applications in 3D object tracking, synthetic aperture imaging, remote sensing, and optical/electron/X-ray microscopy.

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05 gigapixel microscopy using a flatbed scanner

Cited by 33 publications

References 23 publications

A wide field-of-view scanning endoscope for whole anal canal imaging

A wide field-of-view scanning endoscope for whole anal canal imaging

Embedded pupil function recovery for Fourier ptychographic microscopy

Aperture-scanning Fourier ptychography for 3D refocusing and super-resolution macroscopic imaging

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