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Peltoniemi, Kirsi; Vesanto, Erkki; Palva, Airi

doi:10.1007/s00203-002-0411-9

Cited by 30 publications

(8 citation statements)

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“…OppA is an externally exposed extracellular lipoprotein carrying a peptidase II signal for covalent anchoring to the membrane [12] to which diverse roles have been ascribed; it acts as the substrate-binding protein of the oligopeptide transport system in lactobacilli [60], but has also been implicated in cytoadhesion of Mycobacterium hominis and Treponema denticolaria to eukaryotic cells through interaction with plasminogen and fibronectin respectively [61-63]. Furthermore, it has been found to interact with fibronectin and collagen in Lactobacillus casei BL23 and other OppA orthologues from lactobacilli such as MapA from L. reuteri are able to interact with Caco-2 [12,64].…”

Section: Discussionmentioning

confidence: 99%

Surface glycosaminoglycans mediate adherence between HeLa cells and Lactobacillus salivarius Lv72

Martín

Escobedo

et al. 2013

BMC Microbiol

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BackgroundThe adhesion of lactobacilli to the vaginal surface is of paramount importance to develop their probiotic functions. For this reason, the role of HeLa cell surface proteoglycans in the attachment of Lactobacillus salivarius Lv72, a mutualistic strain of vaginal origin, was investigated.ResultsIncubation of cultures with a variety of glycosaminoglycans (chondroitin sulfate A and C, heparin and heparan sulfate) resulted in marked binding interference. However, no single glycosaminoglycan was able to completely abolish cell binding, the sum of all having an additive effect that suggests cooperation between them and recognition of specific adhesins on the bacterial surface. In contrast, chondroitin sulfate B enhanced cell to cell attachment, showing the relevance of the stereochemistry of the uronic acid and the sulfation pattern on binding. Elimination of the HeLa surface glycosaminoglycans with lyases also resulted in severe adherence impairment. Advantage was taken of the Lactobacillus-glycosaminoglycans interaction to identify an adhesin from the bacterial surface. This protein, identify as a soluble binding protein of an ABC transporter system (OppA) by MALDI-TOF/(MS), was overproduced in Escherichia coli, purified and shown to interfere with L. salivarius Lv72 adhesion to HeLa cells.ConclusionsThese data suggest that glycosaminoglycans play a fundamental role in attachment of mutualistic bacteria to the epithelium that lines the cavities where the normal microbiota thrives, OppA being a bacterial adhesin involved in the process.

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Section: Discussionmentioning

confidence: 99%

Surface glycosaminoglycans mediate adherence between HeLa cells and Lactobacillus salivarius Lv72

Martín

Escobedo

et al. 2013

BMC Microbiol

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“…The system was also genetically characterized in L. delbrueckii subsp. bulgaricus, where an extra homolog gene oppA2 is present [241]. The Opp system of other LAB was not widely investigated, but Liu et al [231] found the genes for the Opp system in several strains of L. acidophilus, L. brevis, L. casei, L. rhamnosus, L. johnsonii, L. gasseri, and L. helveticus.…”

Section: Transport System and Peptidasesmentioning

confidence: 99%

Bioprospecting for Bioactive Peptide Production by Lactic Acid Bacteria Isolated from Fermented Dairy Food

2019

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With rapidly ageing populations, the world is experiencing unsustainable healthcare from chronic diseases such as metabolic, cardiovascular, neurodegenerative, and cancer disorders. Healthy diet and lifestyle might contribute to prevent these diseases and potentially enhance health outcomes in patients during and after therapy. Fermented dairy foods (FDFs) found their origin concurrently with human civilization for increasing milk shelf-life and enhancing sensorial attributes. Although the probiotic concept has been developed more recently, FDFs, such as milks and yoghurt, have been unconsciously associated with health-promoting effects since ancient times. These health benefits rely not only on the occurrence of fermentation-associated live microbes (mainly lactic acid bacteria; LAB), but also on the pro-health molecules (PHMs) mostly derived from microbial conversion of food compounds. Therefore, there is a renaissance of interest toward traditional fermented food as a reservoir of novel microbes producing PHMs, and “hyperfoods” can be tailored to deliver these healthy molecules to humans. In FDFs, the main PHMs are bioactive peptides (BPs) released from milk proteins by microbial proteolysis. BPs display a pattern of biofunctions such as anti-hypertensive, antioxidant, immuno-modulatory, and anti-microbial activities. Here, we summarized the BPs most frequently encountered in dairy food and their biological activities; we reviewed the main studies exploring the potential of dairy microbiota to release BPs; and delineated the main effectors of the proteolytic LAB systems responsible for BPs release.

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“…Similar to the oligopeptide transporters found in other Gram-positive bacteria, both the app and opp regions encode an apparent extracellular substrate binding protein (appA and oppA), two apparent integral membrane permeases (appBC and oppBC), and two apparent cytoplasmic ATPbinding proteins (appDF and oppDF). Orthologs of the opp and app oligopeptide transporters are common to many bacterial species, and often, two or more analogous transport systems are encoded by the genomes of Gram-positive bacteria (17,(55)(56)(57)(58). In …”

Section: Identification Of Opp and App Transporters In C Difficilementioning

confidence: 99%

Conserved Oligopeptide Permeases Modulate Sporulation Initiation in Clostridium difficile

2014

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The anaerobic gastrointestinal pathogen Clostridium difficile must form a metabolically dormant spore to survive in oxygenic environments and be transmitted from host to host. The regulatory factors by which C. difficile initiates and controls the early stages of sporulation in C. difficile are not highly conserved in other Clostridium or Bacillus species. Here, we investigated the role of two conserved oligopeptide permeases, Opp and App, in the regulation of sporulation in C. difficile. These permeases are known to positively affect sporulation in Bacillus species through the import of sporulation-specific quorum-sensing peptides. In contrast to other spore-forming bacteria, we discovered that inactivating these permeases in C. difficile resulted in the earlier expression of early sporulation genes and increased sporulation in vitro. Furthermore, disruption of opp and app resulted in greater virulence and increased the amounts of spores recovered from feces in the hamster model of C. difficile infection. Our data suggest that Opp and App indirectly inhibit sporulation, likely through the activities of the transcriptional regulator SinR and its inhibitor, SinI. Taken together, these results indicate that the Opp and App transporters serve a different function in controlling sporulation and virulence in C. difficile than in Bacillus subtilis and suggest that nutrient availability plays a significant role in pathogenesis and sporulation in vivo. This study suggests a link between the nutritional status of the environment and sporulation initiation in C. difficile.

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Cited by 30 publications

References 0 publications

Surface glycosaminoglycans mediate adherence between HeLa cells and Lactobacillus salivarius Lv72

Surface glycosaminoglycans mediate adherence between HeLa cells and Lactobacillus salivarius Lv72

Bioprospecting for Bioactive Peptide Production by Lactic Acid Bacteria Isolated from Fermented Dairy Food

Conserved Oligopeptide Permeases Modulate Sporulation Initiation in Clostridium difficile

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