097 Assay of total- and protein-nitrogen by means of automatic decomposition and coulometric analysis

Kaltenborn, Georg; H�tter, H. J.

doi:10.1007/bf00332091

Cited by 5 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The samples were stored in an airtight box and remixed after thawing before the next measurement. Total fat was determined by the van de Kamer method (1) (double measurements, variation from the mean <7.0%), nitrogen by coulometric titration (3) (triple measurements, variation from the mean <7.5%), and water content by freeze‐drying for 72 hours to constant weight (triple measurements, variation from the mean <1.5%). The accuracy of the results was guaranteed by the use of standard reagents (1,3).…”

Section: Methodsmentioning

confidence: 99%

“…Total fat was determined by the van de Kamer method (1) (double measurements, variation from the mean <7.0%), nitrogen by coulometric titration (3) (triple measurements, variation from the mean <7.5%), and water content by freeze‐drying for 72 hours to constant weight (triple measurements, variation from the mean <1.5%). The accuracy of the results was guaranteed by the use of standard reagents (1,3). For NIRS we used the InfraAlyzer 450 with the IDAS software for spectrum evaluation (BRAN+LUEBBE, Norderstedt, Germany).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous Determination of Fecal Fat, Nitrogen, and Water by Near‐Infrared Reflectance Spectroscopy

Neumeister,

Henker,

Kaltenborn

et al. 1997

J. pediatr. gastroenterol. nutr.

View full text Add to dashboard Cite

BackgroundDeterminations of fecal fat and nitrogen reveal evidence of malabsorption and assist in estimating the efficacy of pancreatic enzyme treatment. Seventy‐two‐hour stool collection, with chemical analysis of fecal fat, and Kjeldahl's method for measurement of fecal nitrogen are generally accepted as standard methods for making these determinations. However, these traditional methods are expensive, timeconsuming, and cumbersome. This study evaluated the efficiency and validity of an alternative method, using nearinfrared reflectance spectroscopy (NIRS) and compared results with those of the standard methods.MethodsNear‐infrared reflectance spectroscopy is a secondary method: The instrument first has to be calibrated with samples analyzed by the standard method. Sixty‐three stool samples with known fat content (range 4.79‐292.5 mg/g), 24 samples with known nitrogen content (range 5.36‐19.38 mg/g), and 24 samples with known water concentration (range 60.1‐82.22%) served for calibration. A further 69 samples were analyzed to validate the procedure.ResultsThere was a satisfactory correlation between the measurements produced by near‐infrared reflectance spectroscopy and those produced by standard methods: fat r = 0.97; nitrogen r = 0.94; water r = 0.96.ConclusionsNear‐infrared reflectance spectroscopy appears to be a reliable, simple, and rapid method of measuring different fecal components‐as precise and accurate as the standard methods. Stool samples should be analyzed immediately after collecting or stored only a few days before analyzing.

show abstract

Section: Methodsmentioning

confidence: 99%