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Abstract

ABSTRACT:Background: Skin wound infected by Staphylococcus aureus (S. aureus) is a common and painful lesion. Tetracycline is commonly used as the antibiotic of choice for its treatment. Aim: to determine the effectiveness of hen egg white lysozyme and dextran conjugated lysozyme in decreasing the bacterial count of experimentally induced skin wound infection in mice. Methods; Lysozyme was conjugated with dextran under mild Maillard reaction conditions. Lysozyme and conjugated lysozyme were tested in vitro for their effectiveness against S. aureus and E. coli. Mouse model of skin wound infection was prepared in 6 group of mices (n=5) by 1cm × 1cm paravertebral laceration, extending to fascia created by scalpel. Five minutes after wounding, 10 µl culture media suspension containing 10 6 CFU⁄cm 2 S. aureus was inoculated. Lysozyme and dextran conjugated lysozyme were applied as a 400µg/ml eucerin ointment base in treatment groups. Tetracycline was applied topically in the third group of mice.

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

“…Staphylococcus aureus is the most common cause of primary and post-operative skin infections, and it has become increasingly resistant to antibiotics, such as methicillin and vancomycin. The use of lysozyme from egg whites and its dextran conjugate was investigated as an alternative topical ointment for the treatment of the infected skin of mice [ 106 ]. The two preparations were tested in vitro against S. aureus and E. coli .…”