[1-11C]Octanoate as a potential PET tracer for studying glial functions: PET evaluation in rats and cats

Kuge, Yuji; Kawashima, Hidefumi; Yamazaki, Shigeaki; Hashimoto, Naoto; Miyake, Yoshihiro

doi:10.1016/s0969-8051(96)00148-5

Cited by 8 publications

(5 citation statements)

References 17 publications

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“…In our experiments, the time course of FFA plasma concentration after an octanoate bolus followed single exponential decay kinetics, in concordance with studies in which radioactively labeled octanoate was injected (33,36). The one-compartment kinetics (37,38) and 2-minute half-life (25,26,37) were similar to these which are reported for LCFA.…”

Section: Discussionsupporting

confidence: 87%

Deconvolution as a Novel Approach to Analyze Moment‐to‐Moment Free Fatty Acid Release

Hücking

Hsu

Ionut

et al. 2007

Obesity

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Obesity. 2007;15:2416-2423. Objective: Recent studies have shown that free fatty acid (FFA) release is pulsatile and that this pattern is controlled by the sympathetic nervous system. It is, then, necessary to understand and characterize adipose tissue lipolysis to elucidate its effect on metabolism. In this study, we introduce deconvolution as a method to detect and quantify pulsatile FFA release. Research Methods and Procedures: Octanoate, a mediumchain fatty acid, was infused in male mongrel dogs (n ϭ 7) to mimic the pulsatile appearance of plasma FFAs. Deconvolution analysis was used to reconstruct the number and timing of infused octanoate pulses from plasma FFA concentrations. Results: Deconvolution analysis was able to reconstruct the exogenously infused pulses of octanoate used to mimic pulsatile appearance of FFAs (pulse frequency, 8 per hour; interpulse interval, 7 minutes). However, determination of pulse mass was less accurate (1.0 Ϯ 0.0 vs. 0.54 Ϯ 0.1 mM). The addition of varying levels of Gaussian noise to non-oscillatory FFA time series did not lead to detection of extraneous FFA pulses. However, goodness of fit declined with increasing variability. Discussion: These results support the use of deconvolution as an accurate approach to determine the temporal sequence of endogenous FFA release.

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Section: Discussionsupporting

confidence: 87%

Deconvolution as a Novel Approach to Analyze Moment‐to‐Moment Free Fatty Acid Release

Hücking

Hsu

Ionut

et al. 2007

Obesity

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“…In rats, 60 min after [ 1 - 11 C]octanoic acid iv injection, high radioactivity was detected in the liver, kidneys, harderian glands, and submaxillary glands. Lower activity was detected in the brain . In cats, activity was rapidly cleared from the blood, while in the brain, there was a transient peak immediately after injection and a second peak 5–10 min later with a gradual decline.…”

Section: Fatty Acidsmentioning

confidence: 99%

“…In cats, activity was rapidly cleared from the blood, while in the brain, there was a transient peak immediately after injection and a second peak 5–10 min later with a gradual decline. In cats, the highest radioactivity was registered in submaxillary glands . In healthy mice, [ 1 - 11 C]octanoic acid was highly retained in kidneys with a fast washout, followed by heart, lung, blood, and liver.…”

Section: Fatty Acidsmentioning

confidence: 99%

“…Lower activity was detected in the brain. 566 In cats, activity was rapidly cleared from the blood, while in the brain, there was a transient peak immediately after injection and a second peak 5−10 min later with a gradual decline. In cats, the highest radioactivity was registered in submaxillary glands.…”

Section: Radiosynthesismentioning

confidence: 99%

“…In cats, the highest radioactivity was registered in submaxillary glands. 566 In healthy mice, [1-11 C]octanoic acid was highly retained in kidneys with a fast washout, followed by heart, lung, blood, and liver. In the liver, it was mainly taken up in parenchymal cells (98% of total liver activity 5 min p.i.…”

Section: Radiosynthesismentioning

confidence: 99%

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Radiosynthesis, Preclinical, and Clinical Positron Emission Tomography Studies of Carbon-11 Labeled Endogenous and Natural Exogenous Compounds

et al. 2022

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The presence of positron emission tomography (PET) centers at most major hospitals worldwide, along with the improvement of PET scanner sensitivity and the introduction of total body PET systems, has increased the interest in the PET tracer development using the short-lived radionuclides carbon-11. In the last few decades, methodological improvements and fully automated modules have allowed the development of carbon-11 tracers for clinical use. Radiolabeling natural compounds with carbon-11 by substituting one of the backbone carbons with the radionuclide has provided important information on the biochemistry of the authentic compounds and increased the understanding of their in vivo behavior in healthy and diseased states. The number of endogenous and natural compounds essential for human life is staggering, ranging from simple alcohols to vitamins and peptides. This review collates all the carbon-11 radiolabeled endogenous and natural exogenous compounds synthesised to date, including essential information on their radiochemistry methodologies and preclinical and clinical studies in healthy subjects.

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Preliminary evaluation of [1-11C]octanoate as a PET tracer for studying cerebral ischemia: A PET study in rat and canine models of focal cerebral ischemia

Kuge

Kawashima²,

Hashimoto

et al. 2000

Ann Nucl Med

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Octanoate is taken up into the brain and is converted in astrocytes to glutamine through the TCA cycle after beta-oxidation. We speculate that [1-11C]octanoate may be used as a tracer for astroglial functions and/or fatty acid metabolism in the brain and may be useful for studying cerebral ischemia. In the present study we investigated brain distribution of [1-11C]octanoate and compared it with cerebral blood flow (CBF) by using rat and canine models of middle cerebral artery (MCA) occlusion and a high resolution PET. In rats brain distribution of [15O]H2O measured 1-2 h and 5-6 h after insult was compared with that of [1-11C]octanoate measured 3-4 h after insult. Radioactivity ratios of lesioned to normal hemispheres determined with [15O]H2O were lower than those determined with [1-11C]octanoate. These results were confirmed by a study on a canine model of MCA-occlusion. Twenty-four hours after insult, CBF decreased in the MCA-territory of the occluded hemisphere, whereas normal or higher accumulation of [1-11C]octanoate was observed in the ischemic regions. The uptake of [1-11C]octanoate-derived radioactivity therefore increased relative to CBF in the ischemic regions, indicating that [1-11C]octanoate provides functional information different from CBF. In conclusion, we found that [1-11C]octanoate is a potential radiopharmaceutical for studying the pathophysiology of cerebral ischemia.

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[1-11C]Octanoate as a potential PET tracer for studying glial functions: PET evaluation in rats and cats

Cited by 8 publications

References 17 publications

Deconvolution as a Novel Approach to Analyze Moment‐to‐Moment Free Fatty Acid Release

Deconvolution as a Novel Approach to Analyze Moment‐to‐Moment Free Fatty Acid Release

Radiosynthesis, Preclinical, and Clinical Positron Emission Tomography Studies of Carbon-11 Labeled Endogenous and Natural Exogenous Compounds

Preliminary evaluation of [1-11C]octanoate as a PET tracer for studying cerebral ischemia: A PET study in rat and canine models of focal cerebral ischemia

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