1,25-Dihydroxyvitamin D3-stimulated mRNAs in rat small intestine

Kessler, Mark A.; Lamm, LO; Jarnagin, Kurt; DeLuca, Hector F.

doi:10.1016/0003-9861(86)90346-2

Cited by 40 publications

(14 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A cDNA library in XgtlO was prepared by using mRNA from the intestine of rats dosed with 1,25-(OH)2D3 12 hr before sacrifice (5). To screen the library, phage were plated on Escherichia coli strain C600HFL.…”

Section: Methodsmentioning

confidence: 99%

“…The second probe was an oligonucleotide, matching the first 20 nucleotides of the 5' end of the published partial sequence (8) 5'-end-labeled with polynucleotide kinase (New England Biolabs) and [a-32P]ATP (5000 Ci/mmol, Amersham). Hybridization and washing conditions using the 160-bp cDNA probe were as previously described (5). When the oligonucleotide was used as the probe, prehybridization was carried out in 6x SSC (0.9 M NaCl/0.09 M sodium citrate)/1 x Denhardt's solution (0.02% bovine serum albumin/0.02% Ficoll/0.02% polyvinylpyrrolidone)/0.5% sodium dodecyl sulfate/0.05% sodium pyrophosphate containing salmon sperm DNA at 10 ,g/ml for 3 hr at 42°C.…”

Section: Methodsmentioning

confidence: 99%

“…This involves the binding of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], the hormonal form of the vitamin, to a highaffinity, low-abundance, intracellular receptor (1), localization of the receptor-ligand complex in the nucleus (2), and the subsequent interaction of the complex with some specific sites on the chromatin, resulting in gene activation (3)(4)(5) The isolation and sequencing of a full-length cDNA is required for a determination of the gene structure and for a study of the mechanism of its regulation. In addition, it has a valuable potential use in a study of the function of CaBP in the intestine.…”

Section: Introductionmentioning

confidence: 99%

“…This involves the binding of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], the hormonal form of the vitamin, to a highaffinity, low-abundance, intracellular receptor (1), localization of the receptor-ligand complex in the nucleus (2), and the subsequent interaction of the complex with some specific sites on the chromatin, resulting in gene activation (3)(4)(5). Consequently, an understanding of the nature of the molecular events resulting in gene activation by 1,25-(OH)2D3 is of central importance in the understanding of the mechanism of action of vitamin D. The isolation of a structural gene responsive to 1,25-(OH)2D3 is essential.…”

mentioning

confidence: 99%

“…At least one such gene is that which encodes the intestinal calcium-binding protein (ICaBP). A number of vitamin D-responsive cDNA clones have been isolated from a rat intestinal library by differential hybridization (5). Among the identified clones was the rat ICaBP (Mr 9000).…”

mentioning

confidence: 99%

See 4 more Smart Citations

Molecular cloning of the cDNA and chromosomal gene for vitamin D-dependent calcium-binding protein of rat intestine.

Darwish¹,

Krisinger²,

Strom³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

A cDNA encoding the vitamin D-dependent rat intestinal calcium-binding protein has been isolated by screening a rat intestinal cDNA library. The cDNA is 406 nucleotides long and appears to contain all the sequences of the mRNA. The cDNA includes the entire protein coding region. It consists of 237 nucleotides coding for 79 amino acids, including the starting methionine, flanked by 62 and 107 noncoding nucleotides at the 5' and 3' ends, respectively. Using the cloned cDNA, we have isolated a genomic clone from a rat liver genomic library. Restriction mapping and Southern analysis using synthetic oligonucleotides localized the gene to a 4.0-kilobase-pair HindIII fragment.The mechanism of action of vitamin D apparently follows a pattern similar to that of the other steroid hormones. This involves the binding of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], the hormonal form of the vitamin, to a highaffinity, low-abundance, intracellular receptor (1), localization of the receptor-ligand complex in the nucleus (2), and the subsequent interaction of the complex with some specific sites on the chromatin, resulting in gene activation (3)(4)(5) The isolation and sequencing of a full-length cDNA is required for a determination of the gene structure and for a study of the mechanism of its regulation. In addition, it has a valuable potential use in a study of the function of CaBP in the intestine. A partial nucleotide sequence of the rat ICaBP cDNA and partial amino acid sequence have been reported (8). MacManus et al. have reported a complete ICaBP amino acid sequence from a purified protein (9). However, this sequence lacked the starting methionine residue at the amino terminus. The missing methionine was detected when an amino-terminal peptide obtained from the product of in vitro translation of the intestinal mRNA was sequenced (10). No larger precursor for the protein has been detected in vivo or in vitro.In the present work we report the isolation of a full-length rat ICaBP cDNA and determination of its nucleotide sequence. The primary structure of the protein is also described. A genomic recombinant clone that contains the rat ICaBP gene has also been isolated.t MATERIALS AND METHODScDNA Library Screening for Rat ICaBP. A cDNA library in XgtlO was prepared by using mRNA from the intestine of rats dosed with 1,25-(OH)2D3 12 hr before sacrifice (5). To screen the library, phage were plated on Escherichia coli strain C600HFL. Hybridization was performed with two probes on duplicate filters. The first probe was a 160-base-pair (bp) cDNA fragment representing the 3' end of the ICaBP cDNA and nick-translated with [a-32P]dCTP (3000 Ci/mmol, Amersham; 1 Ci = 37 GBq). The second probe was an oligonucleotide, matching the first 20 nucleotides of the 5' end of the published partial sequence (8) 5'-end-labeled with polynucleotide kinase (New England Biolabs) and [a-32P]ATP (5000 Ci/mmol, Amersham). Hybridization and washing conditions using the 160-bp cDNA probe were as previously described (5). When the oligonucleotide was u...

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…This involves the binding of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], the hormonal form of the vitamin, to a highaffinity, low-abundance, intracellular receptor (1), localization of the receptor-ligand complex in the nucleus (2), and the subsequent interaction of the complex with some specific sites on the chromatin, resulting in gene activation (3)(4)(5). Consequently, an understanding of the nature of the molecular events resulting in gene activation by 1,25-(OH)2D3 is of central importance in the understanding of the mechanism of action of vitamin D. The isolation of a structural gene responsive to 1,25-(OH)2D3 is essential.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Molecular cloning of the cDNA and chromosomal gene for vitamin D-dependent calcium-binding protein of rat intestine.

Darwish¹,

Krisinger²,

Strom³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

Isolation and characterization of all-trans-retinoic acid-responsive genes in the rat testis

Gaemers

Pelt²,

Themmen

et al. 1998

Mol. Reprod. Dev.

View full text Add to dashboard Cite

By way of differential screening of testis cDNA libraries from vitamin A–deficient (VAD) rats before and after administration of all‐trans retinoic acid (ATRA), genes, the transcription of which was influenced by ATRA, were isolated. Most clones with an increased transcription encoded different subunits of the same mitochondrial protein complex, cytochrome c oxidase (COX). The mRNA expression of COX increased by a factor 3.9±1.5 (mean±SD, n= 4). This increased expression seems to reflect an increased energy demand in the ATRA‐supplemented VAD testis. Also, one gene was isolated, the transcription of which was reduced to about 70% by ATRA. This gene, sulfated glycoprotein 2 (Sgp‐2), is a major secretion product of Sertoli cells, the function of which is still unknown. The effect of ATRA on Sgp‐2 expression may be direct, since the promoter of Sgp‐2 contains a putative ATRA‐responsive element (RARE). Mol. Reprod. Dev. 50:1–6, 1998. © 1998 Wiley‐Liss, Inc.

show abstract

Regulation of calbindin-D28K gene expression by 1,25-dihydroxyvitamin D3 in chick kidney

Hall

Norman

1990

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

We report here the use of a cloned cDNA for the avian calbindin-D28K (28 kD, vitamin D-dependent calcium binding protein, CaBP) to investigate the expression of the chick calbindin gene in the kidney. All three cal-bindin-D28K mRNA species (2000, 2600, and 3100 nucleotide transcripts) were present in the kidney tissue of chronically vitamin D-deficient (-D) chicks; this basal constitutive level of expression was, however, enhanced by administration of the vitamin D3 metabolite, 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in both a time- and dose-dependent manner. D-deficient chick renal calbindin-D28K protein levels (measured by ELISA) were maximally (twofold) stimulated by 6.5 nmole per animal of 1,25-(OH)2D3 when measured 48 h later; a concomitant level of augmentation of calbindin-D28K mRNA accumulation was also observed at this time. Time course experiments showed that enhanced renal calbindin-D28K mRNA accumulation (in -D chicks) was significantly stimulated as early as 8 h and were maximal 12 h after a single pharmacologic dose of 1,25-(OH)2D3; this elevated level of gene expression was maintained for at least 72 h. Renal calbindin-D28K protein levels (constitutively expressed in the -D chick) were significantly stimulated (twofold) as early as 12 h following the single dose of steroid hormone; the level of calbindin-D28K also remained elevated for a minimum of 72 h. Collectively, these data indicate that 1,25-(OH)2D3 acts upon the renal calbindin-D28K gene in a manner similar to that operable in the intestine.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

1,25-Dihydroxyvitamin D3-stimulated mRNAs in rat small intestine

Cited by 40 publications

References 30 publications

Molecular cloning of the cDNA and chromosomal gene for vitamin D-dependent calcium-binding protein of rat intestine.

Molecular cloning of the cDNA and chromosomal gene for vitamin D-dependent calcium-binding protein of rat intestine.

Isolation and characterization of all-trans-retinoic acid-responsive genes in the rat testis

Regulation of calbindin-D28K gene expression by 1,25-dihydroxyvitamin D3 in chick kidney

Contact Info

Product

Resources

About