1,25-Dihydroxyvitamin D3 stimulates specifically the last steps of epidermal differentiation of cultured human keratinocytes

Régnier, M; Daemon, Michel

doi:10.1111/j.1432-0436.1991.tb00235.x

Cited by 45 publications

(22 citation statements)

References 60 publications

(71 reference statements)

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“…Likewise, 1,25-dihydroxyvitamin D 3 , which exerts changes in epidermal differentiation and proliferation (38,39), had no effect on barrier development. This lack of effects does not exclude a role for one or more of these compounds in epidermal development.…”

Section: Discussionmentioning

confidence: 99%

Activators of the nuclear hormone receptors PPARalpha and FXR accelerate the development of the fetal epidermal permeability barrier.

Hanley¹,

Jiang²,

Crumrine³

et al. 1997

J. Clin. Invest.

157

124

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Section: Discussionmentioning

confidence: 99%

Activators of the nuclear hormone receptors PPARalpha and FXR accelerate the development of the fetal epidermal permeability barrier.

Hanley¹,

Jiang²,

Crumrine³

et al. 1997

J. Clin. Invest.

157

124

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“…The kératinocytes used can be either obtained from dissociated interfollicular epi dermis [ 1,6] or derived from the outgrowth of the hair follicle outer root sheath kératino cytes [7], In both cases, the resulting epider mis is stratified showing the various epider mal cell layers including a compact stratum corneum [8]. Reconstructed epidermis (REp) resembles normal epidermis with respect to morphological as well as biochemical criteria and is responsive to hormones such as reti noic acid [9][10][11] and 1,25-dihydroxyvitamin D3 [12],…”

mentioning

confidence: 99%

Reconstructed Human Epidermis: A Model to Study in vitro the Barrier Function of the Skin

Régnier

Caron

Reichert

et al. 1992

Skin Pharmacol Physiol

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In previous studies, we have shown that architecture and differentiation of human epidermis reconstructed by culturing dissociated keratinocytes on deepidermized dermis at the air-liquid interface resembles very much those of epidermis in vivo. The various types of epidermal layers are present with the appropriate differentiation markers, such as the bullous pemphigoid antigen, suprabasal keratins, involucrin, membrane-bound transglutaminase and filaggrin, positioned almost like in normal epidermis. At the ultrastructural level, heterogeneous keratohyalin granules and intra- and extracellular membrane-coating granules are observed. After 7 days of culture, a compact stratum corneum covers the reconstructed epidermis. In the present work, the barrier function of the reconstructed epidermis was evaluated by measuring fluxes of ³H-water. Our results show that under the various culture conditions tested, the presence of the reconstructed epidermis reduces dramatically the permeability of the deepidermized dermis although the skin equivalent exhibits a higher permeability than normal skin. Conditions that favor terminal differentiation of the keratinocytes such as maintaining the cultures in delipidized serum improve the barrier function. Reduction of the relative humidity has a similar effect, whereas the age of the culture is of no influence. Experiments are in progress to reconstruct human epidermis with a barrier function as efficient as in normal skin.

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“…The penetration of vitamin D 3 analogues into the upper differentiated cell layers could itself explain the antiparakeratotic effect, since the differentiation promoting action of vitamin D 3 involves the last stages of keratinocyte differentiation [23]. The terminal differentiation involves the cell membrane on the one hand and the keratinization on the other.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of the Antiparakeratotic Potency of Calcitriol and Tacalcitol in Liposomal Preparations in the Mouse Tail Test

Körbel

Sebők

Kerényi

et al. 2001

Skin Pharmacol Physiol

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In order to test the advantage of vitamin D₃ preparations in liposomal form, calcitriol, the natural activated form of vitamin D₃, and tacalcitol, a vitamin D₃ analogue, were employed in various concentrations and using different vehicles in the mouse tail test, an animal model for testing the antiparakeratotic efficacy of topical medications. The optimal concentration in petrolatum turned out to be similar to that in commercial preparations. The liposomal preparations were superior to those in petrolatum and to those in nonliposomal phospholipids. The antiparakeratotic potency (drug activity) of liposomal tacalcitol in a concentration of 2 µg/g was twice that of the commercial preparation with a higher concentration of 4 µg/g. These results suggest that the use of liposomal vitamin D₃ preparations can achieve a given antipsoriatic effect with a reduced concentration of the active substance thereby reducing the risk of skin irritation and of hypercalcemia.

show abstract

1,25-Dihydroxyvitamin D3 stimulates specifically the last steps of epidermal differentiation of cultured human keratinocytes

Cited by 45 publications

References 60 publications

Activators of the nuclear hormone receptors PPARalpha and FXR accelerate the development of the fetal epidermal permeability barrier.

Activators of the nuclear hormone receptors PPARalpha and FXR accelerate the development of the fetal epidermal permeability barrier.

Reconstructed Human Epidermis: A Model to Study in vitro the Barrier Function of the Skin

Enhancement of the Antiparakeratotic Potency of Calcitriol and Tacalcitol in Liposomal Preparations in the Mouse Tail Test

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