1,3‐propanediol production from glucose by mixed‐culture fermentation of Zygosacharomyces rouxii and Klebsiella pneumonia

Abstract

1,3‐propanediol is an important chemical widely used in polymer production. In this study, two strains, Zygosacharomyces rouxii JL2011 and Klebsiella pneumoniae S6, were used as a mixed culture for 1,3‐propanediol production directly from glucose. Two important parameters including inoculation time of K. pneumoniae S6 at stage of mixed culture and initial cell ratio of Z. rouxii JL2011 to K. pneumoniae S6 in mixed fermentation were optimized in culture flasks. In those experiments, the best results were obtained with a yield of 6.8 g/L 1,3‐propanediol from glucose when K. pneumoniae S6 was inoculated after 48 h in the culture of Z. rouxii JL2011 by mixed culture of Z. rouxii JL2011 and K. pneumoniae S6 with initial cell ratio of 1:200. In a 7‐L bioreactor, the maximum 1,3‐propanediol production could reach up to 15.2 g/L. Thus, this study presents an effective process for 1,3‐propanediol microbial production from glucose by using mixed culture of Z. rouxii JL2011 and K. pneumoniae S6. This work does not only demonstrate a new way to produce 1,3‐propanediol from a low‐cost feedstock, but may also make a valuable contribution to the development of a cost‐effective fermentation based on renewable resources.

The quest for sustainable polyesters – insights into the future

“…To overpass such challenges, different strategies have been attempted, namely the use of mixed microorganism cultures or the utilization of genetically engineered microorganisms that are able to transform glucose into glycerol, followed by the conversion of the latter into PDO. 72,73 Currently, PDO is being produced at an industrial level by the aerobic fermentation of glucose (from corn starch), by the joint venture DuPont™ and Tate&Lyle LLC, with an annual production of ca. 45 000 tons.…”

Current advances in microbial production of 1,3‐propanediol

“…Conversion of low‐cost raw glucose to 1,3‐PDO can also be achieved by using microbial co‐culture. For instance, 1,3‐PDO production from glucose was achieved by co‐culture Zygosacharomyces rouxii JL2011 and K. pneumoniae S6 with a maximum yield of 15.2 g L −1 126 . The co‐cultivation strategy is therefore expected to convert cheap substances such as glucose or CO 2 into 1,3‐PDO.…”

Techno-Economic and Environmental Assessments of 1,3-Propanediol and Xylooligosaccharide Production Annexed to a Typical Sugar Mill

“…XOS are short-chained oligomers of xylose sugar units with several documented health benefits, which can be produced with relatively simple bioprocesses. Due to their prebiotic effects on the beneficial bacteria in the large intestine, XOS is used in animal feed and food products. , The growth in the prebiotics market drives an increase in the XOS market at a CAGR of 4.1% to approximately USD 130 million in 2025 . Additionally, at a market price of USD 25–40/kg XOS, the price per dose is highly competitive with other well-known prebiotics such as glucooligosaccharides and fructooligosaccharides, leaving XOS with considerable growth potential in the 934.13 kt/yr prebiotics market .…”