1-(4-Amino-2-Hydroxyphenyl)Ethenone Suppresses Agrobacterium tumefaciens Virulence and Metabolism

Abstract

The impact of 1-(4-amino-2-hydroxyphenyl)ethanone (AHPE) from the metabolites of endophytic fungus Phomopsis liquidambari on quorum sensing (QS) of Agrobacterium tumefaciens was evaluated for the first time in this study. Exposure to AHPE at concentrations ranging from 12.5 to 50 μg/mL, the β-galactosidase activity, acyl-homoserine lactone level, swimming motility, chemotaxis, and flagella formation were significantly inhibited. qRT-PCR quantification combined with the docking analysis demonstrated that AHPE affected the QS system of A. tumefaciens by repressing the transcriptional levels of traI and traR rather than signal mimicry. 1 H NMR-based metabolic analysis indicated that the metabolism of A. tumefaciens was notably disturbed with AHPE treatment. AHPE treatment also resulted in the enhanced oxidative stress in A. tumefaciens . The enhanced oxidative stress lead to the disorder of energy supply, protein synthesis, and nucleotide metabolism, and ultimately attenuated the pathogenicity of A. tumefaciens . Our study indicated that AHPE can serve as a potential pesticide to defend against A. tumefaciens .

Ethnopharmacological Survey and Comparative Study of the Healing Activity of Moroccan Thyme Honey and Its Mixture with Selected Essential Oils on Two Types of Wounds on Albino Rabbits

“…Likewise, the add-on topical camphor therapy with honey and sesame oil resulted in a significant acceleration in the healing, epithelization, and neovascularization in rats in experimental models of second-degree deep burn wound [ 91 ]. Cyclopentadienyl complexes [ 92 ] and ethanone derivatives [ 93 ] have also been explored as potent antimicrobial, antioxidant, and anticancer compounds via inhibition of reactive oxygen species and free radical production. Terpinenes (e.g., g- and α-terpinene and terpinen-4-ol) have also shown excellent antioxidant, analgesic, anti-inflammatory, anti-proliferative, and antimicrobial effects [ 94 ].…”

Characterization of the antagonistic potential of the glyphosate-tolerant Pseudomonas resinovorans SZMC 25872 strain against the plant pathogenic bacterium Agrobacterium tumefaciens

“…plantarum 32a resulted in a 79.1-87.5% reduction of the symptoms caused by the A. tumefaciens C58 and B6 isolates ( Abdallah et al., 2018b ). Metabolites, such as 1-(4-amino-2-hydroxyphenyl)ethanone produced by P. liquidambari as well as phenylacetic acid and behenic acid produced by B. megaterium L2 were found to inhibit the growth of A. tumefaciens in the studies of Zhou et al. (2020) and Xie et al.…”

“…A. tumefaciens possesses a serious challenge in pest management as it can infect 93 families, 331 genera, and 643 species including both monocotyledonous and dicotyledonous plants ( Conner and Dommisse, 1992 ; Xie et al., 2021 ). The disease causes plant growth inhibition and yield losses, furthermore, severe cases can result in plant death ( Filo et al., 2013 ; Zhou et al., 2020 ). Other plants can also be easily infected; therefore, disease management represents a serious concern ( Dandurishvili et al., 2011 ).…”

“…(2013) proposed biological control as the most efficient technique to control crown-gall disease. Strains of a variety of microbial species, such as Pseudomonas fluorescens, Bacillus amyloliquefaciens , B. subtilis , and Phomopsis liquidambari were also reported to be effective for the biological control of A. tumefaciens ( Hammami et al., 2008 ; Dandurishvili et al., 2011 ; Abdallah et al., 2018a ; Zhou et al., 2020 ).…”

Virulence and biofilm inhibition of 3-methoxycinnamic acid against Agrobacterium tumefaciens

“…The thallus was collected and washed with pre‐cooled PBS three times. Except for vir G (Zhou et al, 2020), Primer Premier 5.0 software was used to design all remaining primers. The primers were listed in Table S1.…”