1,4-Naphthoquinone accumulates reactive oxygen species in Staphylococcus aureus: a promising approach towards effective management of biofilm threat

Paul, Payel; Chakraborty, Poulomi; Chatterjee, Ahana; Sarker, Ranojit Kumar; Dastidar, Debabrata Ghosh; Kundu, Tapanendu; Sarkar, Niloy; Das, Amlan; Tribedi, Prosun

doi:10.1007/s00203-020-02117-1

Cited by 31 publications

(14 citation statements)

References 34 publications

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“…Therefore, the development of microbial biofilm-attenuating agents provides an effective way to prevent and treat such infections. According to reports, the antimicrobial peptide 1,4-naphthoquinone can inhibit the biofilm formation of the Gram-positive bacterium S. aureus through the accumulation of cellular ROS ( 27 ). The antimicrobial peptide AMPNT-6 can effectively prevent attachment and biofilm formation by the Gram-negative bacterium Shewanella putrefaciens ( 28 ).…”

Section: Discussionmentioning

confidence: 99%

The Antimicrobial Peptide MPX Can Kill Staphylococcus aureus, Reduce Biofilm Formation, and Effectively Treat Bacterial Skin Infections in Mice

Zhu

Zhao

et al. 2022

Front. Vet. Sci.

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Staphylococcus aureus is a common pathogen that can cause pneumonia and a variety of skin diseases. Skin injuries have a high risk of colonization by S. aureus, which increases morbidity and mortality. Due to the emergence of multidrug-resistant strains, antimicrobial peptides are considered to be among the best alternatives to antibiotics due to their unique mechanism of action and other characteristics. MPX is an antibacterial peptide extracted from wasp venom that has antibacterial activity against a variety of bacteria. This study revealed that MPX has good bactericidal activity against S. aureus and that its minimum inhibitory concentration (MIC) is 0.08 μM. MPX (4×MIC) can kill 99.9% of bacteria within 1 h, and MPX has good stability. The research on the bactericidal mechanism found that MPX could destroy the membrane integrity, increase the membrane permeability, change the membrane electromotive force, and cause cellular content leakage, resulting in bactericidal activity. Results from a mouse scratch model experiment results show that MPX can inhibit colonization by S. aureus, which reduces the wound size, decreases inflammation, and promotes wound healing. This study reports the activity of MPX against S. aureus and its mechanism and reveals the ability of MPX to treat S. aureus infection in mice, laying the foundation for the development of new drugs for bacterial infections.

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Section: Discussionmentioning

confidence: 99%

The Antimicrobial Peptide MPX Can Kill Staphylococcus aureus, Reduce Biofilm Formation, and Effectively Treat Bacterial Skin Infections in Mice

Zhu

Zhao

et al. 2022

Front. Vet. Sci.

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“…ChemMedChem Lawsone derivatives À OH C-2 S. aureus [24] Arylamino-1,4-naphthoquinone derivatives grafted with Scyclohexane and arylamide or aliphatic amide moieties S-cyclohexane and arylamide or aliphatic amide moieties C-2 C-3 S. aureus [38] 1,4-NQ No side group No side group S. aureus [61] Juglone À OH C-5 S. aureus [62] of DNA gyrase…”

Section: S Aureusmentioning

confidence: 99%

“…Menadione was found to enhance oxidative stress in S. aureus at an MIC of 64-256 μg/mL and MBC of 64-512 μg/mL after 6 h with effects lasting up to 12 h. 1,4-NQ based compounds can effectively inhibit biofilm formation in target organisms, mainly due to the accumulation of cellular ROS. [61] There are two possible mechanisms for the production of oxidative stress. One is that quinones as potent electrophiles react with the thiol group of glutathione and reduce it, resulting in the development of oxidative stress.…”

Section: Reactive Oxygen Species Generationmentioning

confidence: 99%

“…Biofilms can be targeted by inhibiting their formation or disrupting preformed biofilms. [75] Paul et al [61] evaluated the effect of 1,4-NQ (5 and 10 μg/mL) on biofilms and reported inhibition of biofilm-formation in S. aureus at concentrations of 1/20 MBC and 1/10 MBC (MBC: 100 μg/mL). Mone et al [14] reported that menadione was effective against different MDR strains of S. aureus not only through inhibition of biofilm formation but also preformed biofilms at MICs ranging from 64-256 μg/mL.…”

Section: Biofilm Inhibitionmentioning

confidence: 99%

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How Structure–Function Relationships of 1,4‐Naphthoquinones Combat Antimicrobial Resistance in Multidrug‐Resistant (MDR) Pathogens

et al. 2022

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Antimicrobial resistance (AMR) is one of the top ten health‐related threats worldwide. Among several antimicrobial agents, naphthoquinones (NQs) of plant/chemical origin possess enormous structural and functional diversity and are effective against multidrug‐resistant (MDR) pathogens. 1,4‐NQs possess alkyl, hydroxyl, halide, and metal groups as side chains on their double‐ring structure, predominantly at the C‐2, C‐3, C‐5, and C‐8 positions. Among 1,4‐NQs, hydroxyl groups at either C‐2 or C‐5 exhibit significant antibacterial activity against Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. (ESKAPE) and MDR categories. 1,4‐NQs exhibit antibacterial activities like plasmids curing, reactive oxygen species generation, efflux pumps inhibition, anti‐DNA gyrase activity, membrane permeabilization, and biofilm inhibition. This review emphasizes the structure‐function relationships of 1,4‐NQs against ESKAPE and MDR pathogens based on a literature review of studies published in the last 15 years. Overall, 1,4‐NQs have great potential for counteracting the antimicrobial resistance of MDR pathogens.

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“…In 2021, Paul et al 141 tested the antibacterial and anti-biolm activity of 1,4-naphthoquinone (44) against Staphylococcus aureus (Fig. 23).…”

Section: Targeting Biolmsmentioning

confidence: 99%

Bacterial virulence factors: a target for heterocyclic compounds to combat bacterial resistance

et al. 2021

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1,4-Naphthoquinone accumulates reactive oxygen species in Staphylococcus aureus: a promising approach towards effective management of biofilm threat

Cited by 31 publications

References 34 publications

The Antimicrobial Peptide MPX Can Kill Staphylococcus aureus, Reduce Biofilm Formation, and Effectively Treat Bacterial Skin Infections in Mice

The Antimicrobial Peptide MPX Can Kill Staphylococcus aureus, Reduce Biofilm Formation, and Effectively Treat Bacterial Skin Infections in Mice

How Structure–Function Relationships of 1,4‐Naphthoquinones Combat Antimicrobial Resistance in Multidrug‐Resistant (MDR) Pathogens

Bacterial virulence factors: a target for heterocyclic compounds to combat bacterial resistance

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