1,5-Iodonaphthyl azide-inactivated V3526 protects against aerosol challenge with virulent venezuelan equine encephalitis virus

Gupta, Pratiksha; Sharma, Anuj; Spurgers, Kevin B.; Bakken, Russell R.; Eccleston, Lori T.; Cohen, Jeffrey W.; Honnold, Shelley P.; Glass, Pamela J.; Maheshwari, Radha K.

doi:10.1016/j.vaccine.2016.04.049

Cited by 7 publications

(7 citation statements)

References 19 publications

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“…INA has been shown to inactivate enveloped and non-enveloped viruses, including flaviviruses [17][18][19][20]22]. ZIKV was completely inactivated by INA, including the loss of the infectivity of viral RNA.…”

Section: Discussionmentioning

confidence: 99%

“…In future studies, serial passage of INA-inactivated ZIKV will need to be performed in neonatal mice to confirm complete inactivation. Reduction in the binding of INA-inactivated viruses with their respective antibodies has been described previously [17][18][19][20]22]. INA binds to the cysteine amino acid residues in proteins [24].…”

Section: Discussionmentioning

confidence: 99%

“…Sucrose gradient-purified ZIKV was inactivated by INA as described before [18,20]. Inactivation of ZIKV by INA was verified by five serial passages of the inactivated virus in Vero cells.…”

Section: Zikv Is Inactivated By Inamentioning

confidence: 99%

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Inactivation of Zika Virus by Photoactive Iodonaphthyl Azide Preserves Immunogenic Potential of the Virus

et al. 2019

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Zika virus’s (ZIKV) emergence as a pathogen of significant public health importance has accelerated efforts to develop a ZIKV vaccine. To date, the need for an effective ZIKV vaccine is unmet. In this study, we report inactivation of ZIKV using a hydrophobic photoactive compound: 1, 5 iodonaphthyl azide (INA). 50 and 100 µM of INA completely inactivated ZIKV (INA-ZIKV). Western blot and ELISA analysis show some loss of the binding capacity of INA-iZIKV to anti-ZIKV monoclonal antibodies; however, immunization of mice with INA-iZIKV demonstrated seroconversion and ZIKV-neutralizing antibody response. RNA isolated from INA-iZIKV did not induce productive infection in Vero cells, suggesting inactivation of ZIKV RNA. These results suggest that in the absence of an approved ZIKV vaccine, INA-iZIKV can be pursued as a viable ZIKV vaccine candidate.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Inactivation of Zika Virus by Photoactive Iodonaphthyl Azide Preserves Immunogenic Potential of the Virus

et al. 2019

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“…Experiments using encephalomyocarditis virus (EMCV), a positive-sense RNA virus, suggested that INA may inactivate the viral RNA genome by direct binding [133,141]. INA-inactivation of V3526 was also carried out and intramuscular immunization of mice with INA-inactivated V3526 conferred complete protection against an aerosol challenge with TrD [142].…”

Section: Vaccinesmentioning

confidence: 99%

Current Understanding of the Molecular Basis of Venezuelan Equine Encephalitis Virus Pathogenesis and Vaccine Development

Sharma

Knollmann-Ritschel

2019

Viruses

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Venezuelan equine encephalitis virus (VEEV) is an alphavirus in the family Togaviridae. VEEV is highly infectious in aerosol form and a known bio-warfare agent that can cause severe encephalitis in humans. Periodic outbreaks of VEEV occur predominantly in Central and South America. Increased interest in VEEV has resulted in a more thorough understanding of the pathogenesis of this disease. Inflammation plays a paradoxical role of antiviral response as well as development of lethal encephalitis through an interplay between the host and viral factors that dictate virus replication. VEEV has efficient replication machinery that adapts to overcome deleterious mutations in the viral genome or improve interactions with host factors. In the last few decades there has been ongoing development of various VEEV vaccine candidates addressing the shortcomings of the current investigational new drugs or approved vaccines. We review the current understanding of the molecular basis of VEEV pathogenesis and discuss various types of vaccine candidates.

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“…This model was used successfully to test antivirals against VeeV (Julander et al, 2008a). Additionally, a VeeV vaccine inactivated with 1,5-iodonaphthyl azide V3526 protects against both footpad and aerosol challenge with virulent VeeV in a mouse model (Gupta et al, 2016).…”

Section: Norwalk Virusmentioning

confidence: 99%

Animal Models of Human Viral Diseases

Ruiz

Zumbrun

Nalca

2017

Animal Models for the Study of Human Disease

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1,5-Iodonaphthyl azide-inactivated V3526 protects against aerosol challenge with virulent venezuelan equine encephalitis virus

Cited by 7 publications

References 19 publications

Inactivation of Zika Virus by Photoactive Iodonaphthyl Azide Preserves Immunogenic Potential of the Virus

Inactivation of Zika Virus by Photoactive Iodonaphthyl Azide Preserves Immunogenic Potential of the Virus

Current Understanding of the Molecular Basis of Venezuelan Equine Encephalitis Virus Pathogenesis and Vaccine Development

Animal Models of Human Viral Diseases

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