(1→6)- and (1→3)(1→6)-β-glucans from Lasiodiplodia theobromae MMBJ: Structural characterization and pro-inflammatory activity

Oliveira, Kassandra Sussi Mustafé; Bastiani, Mirela Di; Cordeiro, Lucimara M.C.; Costa, M. F. da; Toledo, Karina Alves; Iacomini, Marcello; Babosa, Aneli M.; Dekker, Robert F. H.; Nascimento, Valéria M. G.

doi:10.1016/j.carbpol.2015.07.060

Cited by 30 publications

(23 citation statements)

References 33 publications

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“…Many microorganisms such as fungi and bacteria were reported to have the ability of β-glucan synthesis using carbon sources. Among them, the filamentous fungus Lasiodiplodia theobromae has received a growing interest by researchers for the biosynthesis of an extracellular β-glucan polymer, namely lasiodiplodan, since this fungus has a high potential for industrial production level [1,4]. In this regard, the tremendous biological characteristics of lasiodiplodan such as anti-proliferative effect on breast cancer cells, hypoglycemic function, and antioxidant activities have made it an attractive biopolymer for commercial applications [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

et al. 2021

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Lasiodiplodan is a β-glucan polymer with different interesting characteristics, including therapeutic properties. It is an extracellular product, which is produced by the filamentous fungus Lasiodiplodia theobromae, using glucose as a substrate. In the present work, the production of lasiodiplodan was studied by the utilization of sugarcane straw as a low-cost carbon source. Glucose-rich sugarcane straw hydrolysate was obtained by a sequential pretreatment with dilute nitric acid (1% v/v) and sodium hydroxide (1% w/v), followed by enzymatic hydrolysis. The fermentation process was conducted by the cultivation of the strain Lasiodiplodia theobromae CCT3966 in sugarcane straw hydrolysate in a shake flask at 28 °C for 114 h. It was found that hydrolysate obtained after enzymatic hydrolysis contained 47.10 gL−1 of glucose. Fermentation experiments of lasiodiplodan synthesis showed that the peak yield and productivity of 0.054 gg−1 glucose consumed and 0.016 gL−1 h−1, respectively, were obtained at 72 h fermentation time. Fungal growth, glucose consumption, and lasiodiplodan production from sugarcane straw hydrolysate presented a similar pattern to kinetic models. The study on the chemical structure of lasiodiplodan produced showed it had a β-glucan construction. The current study revealed that sugarcane straw is a promising substrate for the production of lasiodiplodan.

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Section: Introductionmentioning

confidence: 99%

“…Current efforts for microbial synthesis of lasiodiplodan are based on the utilization of commercial sugars, particularly glucose [4,5,[8][9][10]. These sugars are mostly obtained from unsustainable carbon sources such as food-derived carbohydrates, which strongly compete with food chains [11,12].…”

Section: Introductionmentioning

confidence: 99%

Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

et al. 2021

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“…anti-aging [7], anti-inflammatory [8], hypocholesterolemic [9], antioxidant [10] and antimicrobial potential [11] have been described in the scientific literature on β-glucans. These biomacromolecules can be found in the cell wall of yeasts and filamentous fungi, algae and also in cereal grains such as oats and barley.…”

Section: Introductionmentioning

confidence: 99%

Sulfonation of (1→6)-β-D-Glucan (Lasiodiplodan) and Its Antioxidant and Antimicrobial Potential

Calegari¹,

Santos²,

Teixeira³

et al. 2017

JPP

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Abstract:The objective of this study was to investigate the sulfonation of (1→6)-β-D-glucan (lasiodiplodan) as a potentiating mechanism for biological functionalities. Lasiodiplodan was sulfonated by the chlorosulfonic acid-pyridine method. The modified exopolysaccharide was characterized by FT-IR and 13 C NMR spectroscopy, X-ray diffraction and SEM. Antioxidant activity was assessed by the methods of H 2 O 2 and OH radical removal and reducing power. Antimicrobial potential was evaluated by the broth-microdilution method. Sulfonation resulted in a derivative with DS of 0.24. FT-IR analysis indicated the introduction of sulfonyl groups in the macromolecule structure through specific bands in the regions of 1,240 cm -1 and 810 cm -1 . 13 C NMR analysis suggested that sulfonation occurred at carbon 2 of the glucose residue. Sulfonation led to morphological changes in the structure of the biopolymer resulting in a heterogeneous structure with the presence of fibrils. Derivatization promoted an increase in the antioxidant ability of the macromolecule, with a high OH removal potential (74.32%). Bacteriostatic activity against E. coli (Escherichia coli) and S. enterica (Salmonella enterica) typhimurium and fungicidal activity against C. albicans (Candida albicans) and C. tropicalis (Candida tropicalis) were found in the sulfonated sample. Sulfonation potentiated the antioxidant and antimicrobial activities of the biomacromolecule, suggesting that it is a potentiating mechanism of biological functions.

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“…Na Tabela 4 observa-se diferentes configurações e condições físicas e nutricionais do processo fermentativo na produção de LAS. Embora, os estudos subsequentes tenham praticamente mantido as mesmas condições, uma mudança no substrato com uma alta concentração de 50 g/L de sacarose adicionada com sais de Vogel permitiu obter 2,2 g/L de LAS (OLIVEIRA et al, 2015).…”

Section: Fermentação Submersa Para Produção De Lasiodiplodanaunclassified

“…Várias fontes de carbono foram estudadas e L. theobromae demonstrou ter a capacidade de metabolizar com maior afinidade açúcares de seis carbonos, tais como glicose, maltose, sacarose, frutose, para a produção de LAS. Os dois primeiros açúcares são considerados as melhores fontes de carbono para a produção do biopolímero (CUNHA et al, 2012;OLIVEIRA et al, 2015;KAGIMURA et al, 2015c).…”

Section: Fonte De Carbonounclassified

Produção e caracterização do exopolissacarídeo lasiodiplodana por >i<Lasiodiplodia theobromae>/i< a partir de hidrolisado celulósico do bagaço de cana-de-açúcar

Ascencio¹

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The conversion of lignocellulosic biomass to second generation (2G) sugars is crucial for production of biofuels and value added products. However, due to their recalcitrant nature of lignocellulosic biomass, pretreatment is an essential step to increase in increasing the accessibility of cellulolytic enzymes to hemicellulose and cellulose to obtain fermentable 2G sugars. In this study, sugarcane bagasse was pretreated with nitric acid and sodium hydroxide sequentially. The bagasse submitted to sequential acid-base pretreatment led to the production of cellulosic pulp that retained 92.33% of cellulose and eliminated 70.63% of lignin. The obtained cellulosic pulp was enzymatically hydrolyzed (10% of total solids) by Cellic Ctec 2, Novozyme (Curitiba, Brazil), showed a yield of 72.05%, with 67.89 g/L of glucose. The biotechnological potential of cellulosic hydrolyzate supplemented with soybean and rice bran extracts for lasiodiplodan (LAS) production by Lasiodiplodia theobromae CCT 3966 was evaluated by using DCCR 2 2 to study the carbon/nitrogen relationship, generating mathematical models with 95% confidence levels and p-values less than 0.05. The best conditions with soybean bran supplementation allowed to obtain 16.2 g/L of LAS and 8.34 g/L of cell biomass, while supplementation with rice bran produced 22.03 g/L and 14.03 g/L at 120 h, respectively. Some properties from obtained LAS fractions were characterized, such as high purity, low solubility, pseudoplastic behaviour. In addition, SEM, DSC and GPC techniques were used, demonstrated new characteristics, whereas the XRD and FTIR analyzes demonstrated composition and similar values to those reported in the literature for LAS produced by L. theobromae.

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(1→6)- and (1→3)(1→6)-β-glucans from Lasiodiplodia theobromae MMBJ: Structural characterization and pro-inflammatory activity

Cited by 30 publications

References 33 publications

Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

Sulfonation of (1→6)-β-D-Glucan (Lasiodiplodan) and Its Antioxidant and Antimicrobial Potential

Produção e caracterização do exopolissacarídeo lasiodiplodana por >i<Lasiodiplodia theobromae>/i< a partir de hidrolisado celulósico do bagaço de cana-de-açúcar

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