1-Ephedrine in chloroform as a solvent for silver diethyldithiocarbamate in the determination of arsenic

Kopp, John F.

doi:10.1021/ac60331a042

Cited by 27 publications

(8 citation statements)

References 1 publication

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“…About 1 g of each dry derivative was obtained. Samples (0.1 g) were treated as described by Kopp (1973) and arsenic analyses were carried out in an atomic-absorption spectrophotometer IL 151 equipped with an argon-H2 flame, and with background correction. AsH3 was generated by using NaBH4 tablets (R. Bravi & M. Fittipaldi, unpublished work).…”

Section: Analysis Ofarsenicmentioning

confidence: 99%

Affinity-chromatography purification of alkaline phosphatase from calf intestine

Brenna¹,

Perrella²,

Pace³

et al. 1975

Biochemical Journal

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A crude preparation of alkaline phosphatase (EC 3.1.3.1) from calf intestinal mucosa was purified by affinity chromatography on Sepharose-bound derivatives of arsanilic acid, which was found to be a competitive inhibitor of the enzyme. Three biospecific adsorbents were prepared for the chromatography, and the best results were obtained with a tyraminyl-Sepharose derivative coupled with the diazonium salt derived from 4-(p-aminophenylazo)phenylarsonic acid. Alkaline phosphatase was the only enzyme retained by the affinity column in the absence of Pi. The enzyme eluted by phosphate buffer had a specific activity of about 1200 units per mg of protein at pH 10.0, with 5.5mM-p-nitrophenyl phosphate as the substrate.

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Section: Analysis Ofarsenicmentioning

confidence: 99%

Affinity-chromatography purification of alkaline phosphatase from calf intestine

Brenna¹,

Perrella²,

Pace³

et al. 1975

Biochemical Journal

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“…Only about 0.4% of the 232U is precipitated from the hexavalent state with the barium sulfate. Alternatively, an aliquot of the self-cleaning solution can be electrodeposited and standardized directly by [2][3][4][5][6][7] counting, taking the necessary precautions to correct for incomplete electrodeposition.…”

Section: Resultsmentioning

confidence: 99%

Purification of radioactive tracers for use in high sensitivity alpha spectrometry

Sill¹

1974

Anal. Chem.

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“…It is the most popular spectrophotometric method [14] and dates back to the work of Vašák and Šedivec in 1952 [15]. Since then, it has been continuously refined and modified with regard to different sample matrices [16][17][18][19], optimisation of the chromophoric [17,20,21] and hydride generation methods [22][23][24], the identification of interferences [17,[25][26][27][28] and As speciation [29][30][31][32]. Ultimately, a performance comparable to inductively coupled plasma mass spectrometry [33] was achieved.…”

Section: Introductionmentioning

confidence: 99%

Overestimation of geogenic arsenic in landfill leachate: interference of humic and phosphinic acid in a commercial test kit

Brück

Glatthaar

Weigand

2014

International Journal of Environmental Analytical Chemistry

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Large-scale groundwater contamination by arsenic (As) requires economical yet reliable analytical procedures to monitor the quality of well water and the effluent of As elimination processes. The silver diethyldithiocarbamate (AgDDTC) method provides the basis of a series of colourimetric tests used as a compromise between semi-quantitative field-test kits and expensive instrumental analytics. This study aimed at understanding the significant As overestimation by the AgDDTC method observed in landfill leachate samples. Results of the AgDDTC test were up to threefold the levels obtained by reference analyses. Overestimation broadly agreed with the time course of chemical oxygen demand and total phosphorus in the leachate. We hypothesised that the interference was caused by reduced P species or humic acid (HA), respectively. To test this, As-AgDDTC determinations from As standards were conducted with phosphonic (H 3 PO 3 ) and phosphinic acid (H 3 PO 2 ) as well as two commercial HA as matrix components. While H 3 PO 3 showed no effect, H 3 PO 2 in concentrations of 0.06 to 28 mg P L −1 yielded As recoveries of up to 130%. This was probably due to the evolution of phosphine (PH 3 ) as suggested by the absorption spectra recorded after reacting pure PH 3 with AgDDTC in pyridine. The HA in concentrations of 0.6-320 mg dissolved organic carbon L −1 gave As recoveries of up to 600%, possibly due to a chromophoric reaction of volatile HA digestion products formed during the reduction step. Significant As overestimation in the presence of the above-named compounds may require pre-oxidation of the samples. To recognise interference effects, the acquisition of full absorbance spectra may be recommendable in lieu of fixed wavelength analyses.

show abstract

1-Ephedrine in chloroform as a solvent for silver diethyldithiocarbamate in the determination of arsenic

Cited by 27 publications

References 1 publication

Affinity-chromatography purification of alkaline phosphatase from calf intestine

Affinity-chromatography purification of alkaline phosphatase from calf intestine

Purification of radioactive tracers for use in high sensitivity alpha spectrometry

Overestimation of geogenic arsenic in landfill leachate: interference of humic and phosphinic acid in a commercial test kit

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