1986
DOI: 10.1016/0076-6879(86)21003-4
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[1] Factors affecting production of monoclonal antibodies

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Cited by 19 publications
(8 citation statements)
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“…The efficiency of fusion of splenocytes and myeloma cells is also very low and only a small number of fused cells are able to grow as hybridomas during selection [25]. Finally, the stability of the generated clones is also uncertain and a large fraction of hybridomas stop secreting over time, due to random shedding of chromosomes until they reach a stable genomic configuration [26].…”
Section: Discussionmentioning
confidence: 99%
“…The efficiency of fusion of splenocytes and myeloma cells is also very low and only a small number of fused cells are able to grow as hybridomas during selection [25]. Finally, the stability of the generated clones is also uncertain and a large fraction of hybridomas stop secreting over time, due to random shedding of chromosomes until they reach a stable genomic configuration [26].…”
Section: Discussionmentioning
confidence: 99%
“…One hybridoma was used in the present study (DUPl). It was cloned twice by limiting dilution in the presence of endothelial cell growth supplement (Collaborative Research; Westerwoudt, 1986) then grown in large culture flasks to generate conditioned medium. DUPl was identified as an IgM using a subtyping ELISA (Boehringer).…”
Section: Methodsmentioning
confidence: 99%
“…The mean fusion frequency of 19.75 hybridomas per L0' splenocytes obtained in fusions 6 to 9, compares favourably to the expected fusion frequencies published by Westerwoudt (1986). Loss of chromosomes from hybridomas during the early postfusion period has been reported previously (Kohler, 1980;Goding, 1983;Westerwoudt, 1985), and may account for clone mortality during this period.…”
Section: L80supporting
confidence: 80%
“…Lymphoblastoid cell lines have been used as a fusion partner tn human x human fusions, as have Epstein Barr virus-transformed lymphocytes (Kozbor and Roder, 1983; Wasserman cl gl., 1986;Roder 91 9L,1986). Fusion frequency between human cells is extremely low (Westerwoudt, 1986). It is not possible to grow human hybridomas in laboratory animals to establish peritoneal tumours, and monoclonal antibody yield in cell culture is extremely poor (less than 0.5 pg/rr.l), as is long term stability in culture (Kozbor and Roder, 1983).…”
Section: Mediated Renal Diseasementioning
confidence: 99%
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