1-Hydroxyanthraquinone exhibited antibacterial activity by regulating glutamine synthetase of Staphylococcus xylosus as a virulence factor

Wang, Jinpeng; Qu, Qianwei; Liu, Xin; Cui, Weihong; Yu, Fei; Chen, Xingru; Xing, Xiao-Xu; Zhou, Yonggang; Yang, Yanbei; Bello-Onaghise, God’spower; Chen, Xueying; Li, Xiubo; Li, Yanhua

doi:10.1016/j.biopha.2019.109779

Cited by 14 publications

(10 citation statements)

References 41 publications

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“…In accordance with these results, histological lesions were also relieved to a larger extent in the high and medium dosage groups ( Figure 6E ). In short, according to the results of the four major indexes mentioned above, all the three dosage groups of PSP showed significant antidiarrheal activity in an obvious dose-dependent manner, which is in accordance with other reports ( Wang et al, 2015 ). The minimum effective dose of PSP was determined to be 2,000 mg/kg.…”

Section: Resultssupporting

confidence: 91%

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The Active Ingredients Identification and Antidiarrheal Mechanism Analysis of Plantago asiatica L. Superfine Powder

Dong

et al. 2021

Front. Pharmacol.

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Plantago asiatica L. is a natural medicinal plant that has been widely used for its various pharmacological effects such as antidiarrheal, anti-inflammatory, and wound healing. This study aims to explore the antidiarrheal active ingredients of Plantago asiatica L. that can be used as quality markers to evaluate P. asiatica L. superfine powder (PSP). Molecular docking experiment was performed to identify the effective components of P. asiatica L., which were further evaluated by an established mouse diarrhea model. Na+/K+-ATPase and creatine kinase (CK) activities and the Na+/K+ concentrations were determined. The gene expression of ckb and Atp1b3 was detected. PSP was prepared and evaluated in terms of the tap density and the angle of repose. The structures of PSPs of different sizes were measured by infrared spectra. The active ingredient contents of PSPs were determined by HPLC. The results indicated that the main antidiarrheal components of P. asiatica L. were luteolin and scutellarein that could increase the concentration of Na+ and K+ by upregulating the activity and gene level of CK and Na+/K+-ATPase. In addition, luteolin and scutellarein could also decrease the volume and weight of small intestinal contents to exert antidiarrheal activity. Moreover, as the PSP size decreased from 6.66 to 3.55 μm, the powder tended to be amorphous and homogenized and of good fluidity, the content of active compounds gradually increased, and the main structure of the molecule remained steady. The optimum particle size of PSP with the highest content of active components was 3.55 μm, and the lowest effective dose for antidiarrhea was 2,000 mg/kg. Therefore, the antidiarrheal active ingredients of PSP were identified as luteolin and scutellarein that exert antidiarrheal activity by binding with Na+/K+-ATPase. PSP was successfully prepared and could be used as a new dosage form for the diarrhea treatment.

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Section: Resultssupporting

confidence: 91%

“…The steps of total RNA extraction (Tiangen Biotech (Beijing) Co., Ltd) and cDNA synthesis (Takara) were performed according to the manufacturer's instructions. Primers used in quantitative real-time PCR are listed in Table 1 (Wang et al, 2020).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

The Active Ingredients Identification and Antidiarrheal Mechanism Analysis of Plantago asiatica L. Superfine Powder

Dong

et al. 2021

Front. Pharmacol.

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“…The naturally occurring signaling molecule of bacteria, cyclic dinucleotide 3 ′ ,5 ′ -cyclic diguanylic acid (c-di-GMP), has been investigated to inhibit biofilm formation of S. aureus, and a decrease in the colonization of the pathogen in the mammary glands was shown in a murine mastitis model (44). The alternative drug, 1-hydroxyanthraquinone, was found to have a significant inhibitory action against Staphylococcus xylosus in vitro as well as a reduction in inflammation in the mammary glands of murine models (72).…”

Section: Investigations Of Antimicrobial Compounds Against Biofilm Forming Mastitis Pathogensmentioning

confidence: 99%

“…Most of the so-called in vivo investigations of biofilm in bovine mastitis have used experimental models (mice and sheep), and the majority of these studies focused on anti-biofilm treatment or vaccines against biofilm udder infections (Table 1) (44,69,70,72,(83)(84)(85). Only a few studies investigated and confirmed biofilm in vivo within udder tissue of dairy cows with bovine mastitis.…”

Section: In Vivo Investigations Of Biofilm In Bovine Mastitismentioning

confidence: 99%

Biofilm Research in Bovine Mastitis

et al. 2021

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Bovine mastitis is one of the most important diseases in the dairy industry and has detrimental impact on the economy and welfare of the animals. Further, treatment failure results in increased antibiotic use in the dairy industry, as some of these mastitis cases for unknown reasons are not resolved despite standard antibiotic treatment. Chronic biofilm infections are notoriously known to be difficult to eradicate with antibiotics and biofilm formation could be a possible explanation for mastitis cases that are not resolved by standard treatment. This paper reviews the current literature on biofilm in bovine mastitis research to evaluate the status and methods used in the literature. Focus of the current research has been on isolates from milk samples and investigation of their biofilm forming properties in vitro. However, in vitro observations of biofilm formation are not easily comparable with the in vivo situation inside the udder. Only two papers investigate the location and distribution of bacterial biofilms inside udders of dairy cows with mastitis. Based on the current knowledge, the role of biofilm in bovine mastitis is still unclear and more in vivo investigations are needed to uncover the actual role of biofilm formation in the pathogenesis of bovine mastitis.

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“…During the past few years, an increasing number of human infections have been associated with this species such as urinary tract, pancreatic, cardiac, ophthalmologic, dental, blood stream, erythema chest infections, nodosum as well as otogenic brain abscess, to mention a few [9][10][11][12]. Currently, S.xylosus has become the prevalent cause of mastitis in dairy herds in many countries including America, Finland, Canada, Sweden, Switzerland, Norway, and China [13][14][15][16] (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanism of Staphylococcus Xylosus Resistance Against Tylosin and Florfenicol

Liu

Shu

Cui

et al. 2021

Preprint

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As a result of evolution, certain microbes develop resistance against antimicrobial treatment. The antimicrobial resistance (AMR) threatens the effective prevention and treatment of an ever-increasing range of severe infections caused by microbes such as bacteria, viruses, fungi and other parasites. The resistance of Staphylococcus xylosus (S. xylosus) against antibiotic treatment is one of the major causes of the world-wide antibiotic crisis and has remained to be well understood at the molecular level. In order to fill this gap, we investigated various mutations in the sequence of ribosomal proteins involved cross resistance. We discovered that for the mutant containing the insertion L22 97KRTSAIN98 the minimum inhibitory concentration against both tylosin and florfenicol changed dramatically. To understand this effect on a molecular basis and to further elucidate the role of cross resistance, we computationally constructed the 3D model of the large ribosomal subunit from S. xylosus as well as its complexes with both tylosin and florfenicol. Using all-atom molecular dynamics simulations, we found that unique structural changes in the β hairpin of L22 played a central role of this variant in the development of antibiotic resistance in S. xylosus. In addition, the regulation of protein network also played an essential role in the development of cross resistance in S. xylosus. Our work provides insightful views into the mechanism of S. xylosus resistance which could be useful for the development of the next generation of antibiotics.

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1-Hydroxyanthraquinone exhibited antibacterial activity by regulating glutamine synthetase of Staphylococcus xylosus as a virulence factor

Cited by 14 publications

References 41 publications

The Active Ingredients Identification and Antidiarrheal Mechanism Analysis of Plantago asiatica L. Superfine Powder

The Active Ingredients Identification and Antidiarrheal Mechanism Analysis of Plantago asiatica L. Superfine Powder

Biofilm Research in Bovine Mastitis

Molecular Mechanism of Staphylococcus Xylosus Resistance Against Tylosin and Florfenicol

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