[1] Pilot-scale preparation of hemoglobin solutions

Effects of extreme hemodilution with hemoglobin-based O2 carriers on microvascular pressure

“…MP4 is polyethylene glycol-modified human hemoglobin manufactured by Sangart (San Diego, CA), and its preparation and properties have been described elsewhere (29). ␣␣Hb was made according to the method of Winslow and Chapman (34). Solution properties are given in Table 1.…”

Hemoglobin-based oxygen carriers do not alter platelet functions: study of three chemically modified hemoglobin solutions

“…Purified cell-free human hemoglobin solution (purified free Hb, 79 g/l) was obtained from Pasteur Mérieux Serums et Vaccins (Marcy l'Etoile, France). Three solutions of human hemoglobin prepared according to various chemical modifications were studied: (a) hemoglobin conjugated to macromolecules of dextran benzene tetracarboxylate (Hb Dex-BTC, 85 g/l) [12] and obtained from Pasteur Mérieux Serums et Vaccins; (b) tetrameric hemoglobin cross-linked between α-subunits with bis (3,5-dibromosalicyl) fumarate (αα-Hb, 82 g/l; generously supplied by Dr. A. Alayash, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Md., USA) [13]; and (c) hemoglobin polymerized and cross-linked with o-raffinose (o-raffinose-poly-Hb, 100 g/l; generously provided by Hemosol, Toronto, Canada) [14].…”

Purification and characterization of human hemoglobin: effect of the hemolysis conditions

“…Purification demands attention, since phospholipid components of the red cell membrane are potential toxins if present even in small amount in a product to be administered by intravenous infusion [12]. Anion and cation exchange chromatography have been used to eliminate residual phospholipids [13][14][15][16].…”