10.1007/BF00187724

doi:10.1007/bf00187724

“…Their results suggested that a differentiation of populations has already occurred, mainly according to the host plant, instead of the geographical region where populations are localized and they had reported that there was no grouping of samples collected on different crops in the same state. Among the biotypes of B. tabaci, the B biotypes, in the previous two decades has been distributed widely and caused tremendous losses world wide as a pest and vector of virus diseases [2,10,30,31] . The B biotype has distinctive biological traits, together with esterase and RAPD patterns that showed little variation [2,7,26] .…”

Section: Discussionmentioning

confidence: 99%

“…Among the biotypes of B. tabaci, the B biotypes, in the previous two decades has been distributed widely and caused tremendous losses world wide as a pest and vector of virus diseases [2,10,30,31] . The B biotype has distinctive biological traits, together with esterase and RAPD patterns that showed little variation [2,7,26] . Three distinct bands of sizes viz., 350, 800 bp and 1 kb were produced by B11 primer [32] , similar to that in our study clearly distinct bands of 500 bp and 1 kb were produced by the primer OPA 12, while resolved bands were obtained below 500 bp using OPE 04 primer.…”

Section: Discussionmentioning

confidence: 99%

“…It is broadly polyphagous, feeding on an estimated 900 hosts [1] . Since from 1990's, it has caused escalating problems to economically important field, horticultural crops and ornamental plants [2] . Three types of damage may be caused by whitefly; they include direct damage, indirect damage and virus transmission.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, newer viruses transmitted by whiteflies were also reported and is continuing even today [6] . As early as 1986 [2,7,8] , revealed the existence of a new biotype of B. tabaci named 'B' biotype.…”

Section: Introductionmentioning

confidence: 99%

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Host Plant Mediated Population Variations of Cotton Whitefly Bemisia tabaci Gennadius (Aleyrodidae: Homoptera) Characterized with Random DNA Markers

Perumal¹

2009

American J. of Biochemistry and Biotechnology

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Problem statement: Whitefly, Bemisia tabaci is an important sucking pest of field, horticultural and ornamental plants causing feeding injuries besides spreading disease by acting as a vector of Gemini viruses. The polyphagous nature of the pest makes it as a highly complex species. Approach: The influence of host plants utilized by the species on the population differences at molecular level was attempted using Random Amplified Polymorphic DNA (RAPD) markers. Results: Ten RAPD primers out of the total seventeen primers screened produced 236 markers. The total number of bands obtained from each primer ranged from 11-35 with an average of 23.60 bands per primer. Of the pair wise combination among thirteen species, Srivilliputhur population showed the highest similarity index (0.826) while the lowest (0.111) was recorded by Namakkal population. The similarity coefficient based on the 236 RAPD markers generated ranged from 0.111-0.826. Three major clusters were formed from UPGMA dendrogram, which was constructed based on Jaccard's similarity. PCR screening demarcated the whitefly population based on the host species. The first cluster included population collected from okra and cotton, while second cluster comprised of population from eggplant and cauliflower and the third cluster included population from eggplant. It could be deduced that population from cotton and okra had 50% similarity, while 60-70% similarity was observed for population from eggplant and cauliflower. Conclusion: Our investigation offered the lead that within a narrow geographical region there exits variation based on host plants being utilized by the whitefly population.

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“…Moreover, newer viruses transmitted by whiteflies were also reported and is continuing even today [6] . As early as 1986 [2,7,8] , revealed the existence of a new biotype of B. tabaci named 'B' biotype.…”

Section: Introductionmentioning

confidence: 99%

Host Plant Mediated Population Variations of Cotton Whitefly Bemisia tabaci Gennadius (Aleyrodidae: Homoptera) Characterized with Random DNA Markers

Perumal¹,

Marimuthu²,

Salim³

et al. 2009

American Journal of Biochemistry and Biotechnology

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Problem statement: Whitefly, Bemisia tabaci is an important sucking pest of field, horticultural and ornamental plants causing feeding injuries besides spreading disease by acting as a vector of Gemini viruses. The polyphagous nature of the pest makes it as a highly complex species. Approach: The influence of host plants utilized by the species on the population differences at molecular level was attempted using Random Amplified Polymorphic DNA (RAPD) markers. Results: Ten RAPD primers out of the total seventeen primers screened produced 236 markers. The total number of bands obtained from each primer ranged from 11-35 with an average of 23.60 bands per primer. Of the pair wise combination among thirteen species, Srivilliputhur population showed the highest similarity index (0.826) while the lowest (0.111) was recorded by Namakkal population. The similarity coefficient based on the 236 RAPD markers generated ranged from 0.111-0.826. Three major clusters were formed from UPGMA dendrogram, which was constructed based on Jaccard's similarity. PCR screening demarcated the whitefly population based on the host species. The first cluster included population collected from okra and cotton, while second cluster comprised of population from eggplant and cauliflower and the third cluster included population from eggplant. It could be deduced that population from cotton and okra had 50% similarity, while 60-70% similarity was observed for population from eggplant and cauliflower. Conclusion: Our investigation offered the lead that within a narrow geographical region there exits variation based on host plants being utilized by the whitefly population.

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Biochemical evidence of haplodiploidy in the whiteflyBemisia tabaci

Byrne

¹

,

Devonshire

²

1996

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Polymorphic esterase and acetylcholinesterase alleles in the whitefly Bemisia tabaci were studied using electrophoretic and colorimetric assays. The segregation of these alleles between parental and F1 generations provided unequivocal evidence of haplodiploidy in this pest species. Unmated females, heterozygous at a polymorphic locus, produced a 1:1 ratio of haploid males expressing either of the maternal alleles. Although male offspring were produced by both virgin and mated females, the segregation of alleles showed they were always haploid (hemizygous) for the marker enzymes. Females only arose from fertilized eggs and invariably expressed paternal and maternal alleles.

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10.1007/BF00187724

Cited by 41 publications

References 21 publications

Host Plant Mediated Population Variations of Cotton Whitefly Bemisia tabaci Gennadius (Aleyrodidae: Homoptera) Characterized with Random DNA Markers

Host Plant Mediated Population Variations of Cotton Whitefly Bemisia tabaci Gennadius (Aleyrodidae: Homoptera) Characterized with Random DNA Markers

Host Plant Mediated Population Variations of Cotton Whitefly Bemisia tabaci Gennadius (Aleyrodidae: Homoptera) Characterized with Random DNA Markers

Biochemical evidence of haplodiploidy in the whiteflyBemisia tabaci

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