In order to improve the vibration isolation, environmental adaptability and development efficiency of rubber mounts, a novel rubber mount with adjustable parameters such as stiffness, support height and limit distance was proposed, and its structural composition and working principle were described. And then, a commercial vehicle was taken as the research object to conduct the calculation of mounts stiffness by employing the energy decoupling method and genetic algorithm. On the basis of the influence of cone angle, thickness and height on the ratio of axial-radial stiffness, the detailed structural design of the mounts was carried out, and the relationship between the preloading displacement and axial-radial stiffness was studied by the method of theoretical calculation and test, which made the process of the stiffness adjustment more specific and accurate. Finally, the vibration isolation performance test of the Powertrain Mounting System (PMS) before and after stiffness adjustment was completed. Results show that the adjusted PMS indicates better vibration isolation performance at idle speed and slow acceleration in place, and the new mounts can effectively improve the vibration performance of vehicle, environmental adaptability of mounts and product development efficiency.