100 Years Later, What Is New in Glycerol Bioproduction?

Semkiv, Marta V.; Ruchała, Justyna; Dmytruk, Kostyantyn V.; Sibirny, Andriy А.

doi:10.1016/j.tibtech.2020.02.001

Cited by 35 publications

(34 citation statements)

References 48 publications

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“…Baker’s yeast responds to freeze stress-induced hyperosmotic stress by activating the HOG-MAPK pathway, which induces glycerol accumulation [ 34 – 37 ]. It has been extensively documented that glycerol 3-phosphate dehydrogenase, which is encoded by GPD1 and GPD2 , is the key enzyme in the production of glycerol [ 47 , 48 ]. The strong upregulation of GPD1 under hyperosmotic stress [ 49 , 50 ] is at least partly controlled by the HOG1-MAPK cascade [ 49 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

MAL62 overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker’s yeast in lean dough

Sun

Zhang

et al. 2020

Microb Cell Fact

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Background In Saccharomyces cerevisiae, alpha-glucosidase (maltase) is a key enzyme in maltose metabolism. In addition, the overexpression of the alpha-glucosidase-encoding gene MAL62 has been shown to increase the freezing tolerance of yeast in lean dough. However, its cryoprotection mechanism is still not clear. Results RNA sequencing (RNA-seq) revealed that MAL62 overexpression increased uridine diphosphoglucose (UDPG)-dependent trehalose synthesis. The changes in transcript abundance were confirmed by quantitative reverse transcription–polymerase chain reaction (qRT-PCR) and enzyme activity assays. When the UDPG-dependent trehalose synthase activity was abolished, MAL62 overexpression failed to promote the synthesis of intracellular trehalose. Moreover, in strains lacking trehalose synthesis, the cell viability in the late phase of prefermentation freezing coupled with MAL62 overexpression was slightly reduced, which can be explained by the increase in the intracellular glycerol concentration. This result was consistent with the elevated transcription of glycerol synthesis pathway members. Conclusions The increased freezing tolerance by MAL62 overexpression is mainly achieved by the increased trehalose content via the UDPG-dependent pathway, and glycerol also plays an important role. These findings shed new light on the mechanism of yeast response to freezing in lean bread dough and can help to improve industrial yeast strains.

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Section: Discussionmentioning

confidence: 99%

MAL62 overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker’s yeast in lean dough

Sun

Zhang

et al. 2020

Microb Cell Fact

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“…Baker's yeast responds to freeze stress-induced hyperosmotic stress by activating the HOG-MAPK pathway, which induces glycerol accumulation [34][35][36][37]. It has been extensively documented that glycerol 3-phosphate dehydrogenase, which is encoded by GPD1 and GPD2, is the key enzyme in the production of glycerol [47,48]. The strong upregulation of GPD1 under hyperosmotic stress [49,50] is at least partly controlled by the HOG1-MAPK cascade [49,51].…”

Section: Discussionmentioning

confidence: 99%

MAL62 overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker’s yeast in lean dough

Sun

Zhang

Fan

et al. 2020

Preprint

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Background: In Saccharomyces cerevisiae, alpha-glucosidase (maltase) is a key enzyme in maltose metabolism. In addition, the overexpression of the alpha-glucosidase-encoding gene MAL62 has been shown to increase the freezing tolerance of yeast in lean dough. However, its cryoprotection mechanism is still not clear.Results: RNA sequencing (RNA-seq) revealed that MAL62 overexpression increased uridine diphosphoglucose (UDPG)-dependent trehalose synthesis. The changes in transcript abundance were confirmed by quantitative reverse transcription–polymerase chain reaction (qRT-PCR) and enzyme activity assays. When the UDPG-dependent trehalose synthase activity was abolished, MAL62 overexpression failed to promote the synthesis of intracellular trehalose.Moreover, in strains lacking trehalose synthesis, the cell viability in the late phase of prefermentation freezing coupled with MAL62 overexpression was slightly reduced, which can be explained by the increase in the intracellular glycerol concentration. This result was consistent with the elevated transcription of glycerol synthesis pathway members.Conclusions: The increased freezing tolerance by MAL62 overexpression is mainly achieved by the increased trehalose content via the UDPG-dependent pathway, and glycerol also plays an important role. These findings shed new light on the mechanism of yeast response to freezing in lean bread dough and can help to improve industrial yeast strains.

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“…Since then, significant developments have been achieved in improving the yields from living cells of this yeast and hence this phenomenon is well studied in this organism. Glycerol is synthesized by using the glycolysis intermediate DHAP in two consecutive reactions steps and is catalyzed by enzymes including glycerol-3-phosphate dehydrogenase and glycerol-3-phosphate phosphatase [67]. Glycerol production by Saccharomyces cerevisiae and other organisms such as Candida glycerinogenes and Candida krusei (yeasts), Dunaliella tertiolecta and Dunaliella bardawil (algae), Synechococcus elongatus (cyanobacteria), and many other organisms has been extensively reviewed [67].…”

Section: Plausible Commercial Synthesis Of Glycerol From Algae and Otmentioning

confidence: 99%

“…Glycerol is synthesized by using the glycolysis intermediate DHAP in two consecutive reactions steps and is catalyzed by enzymes including glycerol-3-phosphate dehydrogenase and glycerol-3-phosphate phosphatase [67]. Glycerol production by Saccharomyces cerevisiae and other organisms such as Candida glycerinogenes and Candida krusei (yeasts), Dunaliella tertiolecta and Dunaliella bardawil (algae), Synechococcus elongatus (cyanobacteria), and many other organisms has been extensively reviewed [67]. These techniques are indeed the fruitful consequence of well-studied phenomena such as the mechanism of glycerol metabolism and its transportation in organisms [67].…”

Section: Plausible Commercial Synthesis Of Glycerol From Algae and Otmentioning

confidence: 99%

The Role of Glycerol and Its Derivatives in the Biochemistry of Living Organisms, and Their Prebiotic Origin and Significance in the Evolution of Life

Gull

Pasek

2021

Catalysts

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The emergence and evolution of prebiotic biomolecules on the early Earth remain a question that is considered crucial to understanding the chemistry of the origin of life. Amongst prebiotic molecules, glycerol is significant due to its ubiquity in biochemistry. In this review, we discuss the significance of glycerol and its various derivatives in biochemistry, their plausible roles in the origin and evolution of early cell membranes, and significance in the biochemistry of extremophiles, followed by their prebiotic origin on the early Earth and associated catalytic processes that led to the origin of these compounds. We also discuss various scenarios for the prebiotic syntheses of glycerol and its derivates and evaluate these to determine their relevance to early Earth biochemistry and geochemistry, and recapitulate the utilization of various minerals (including clays), condensation agents, and solvents that could have led to the successful prebiotic genesis of these biomolecules. Furthermore, important prebiotic events such as meteoritic delivery and prebiotic synthesis reactions under astrophysical conditions are also discussed. Finally, we have also highlighted some novel features of glycerol, including glycerol nucleic acid (GNA), in the origin and evolution of the life.

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100 Years Later, What Is New in Glycerol Bioproduction?

Cited by 35 publications

References 48 publications

MAL62 overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker’s yeast in lean dough

MAL62 overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker’s yeast in lean dough

MAL62 overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker’s yeast in lean dough

The Role of Glycerol and Its Derivatives in the Biochemistry of Living Organisms, and Their Prebiotic Origin and Significance in the Evolution of Life

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