1999
DOI: 10.1016/s0076-6879(99)99014-6
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[11] Reversed-phase high-performance liquid chromatography methods for analysis of wine polyphenols

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Cited by 52 publications
(25 citation statements)
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“…Wine phenolic compounds were quantified using a reversedphase high-performance liquid chromatography-photodiode array detection (RP-HPLC-DAD) technique as described by Waterhouse et al (1999) and Minnaar et al (2015). Monomeric anthocyanins, flavan-3-ols, flavonols and phenolic acids were measured at absorbance wavelengths of 520 nm, 280 nm, 360 nm and 316 nm respectively.…”
Section: Wine Phenolic Compoundsmentioning
confidence: 99%
“…Wine phenolic compounds were quantified using a reversedphase high-performance liquid chromatography-photodiode array detection (RP-HPLC-DAD) technique as described by Waterhouse et al (1999) and Minnaar et al (2015). Monomeric anthocyanins, flavan-3-ols, flavonols and phenolic acids were measured at absorbance wavelengths of 520 nm, 280 nm, 360 nm and 316 nm respectively.…”
Section: Wine Phenolic Compoundsmentioning
confidence: 99%
“…Ultra-violet visible spectra were recorded for all peaks. The analytical method is based on the method described by Waterhouse et al (1999) for wine polyphenol separation and quantification. Anthocyanins were detected at 520 nm using malvidin-3-O-glucoside as external standard.…”
Section: Sample Preparationmentioning
confidence: 99%
“…Flavonols were detected at 360 nm using rutin and flavanols at 280 nm using (+)-catechin as external standards. The identification of these compounds was confirmed by their relative retention times and UV-visible absorption characteristics, using the method of Waterhouse et al (1999) …”
Section: Sample Preparationmentioning
confidence: 99%
“…The concentrations were determined using calibration curves and peak areas. The average content was calculated as a mean of 3 different readings obtained at different seasons 15 .…”
Section: Hplc Analysismentioning
confidence: 99%