111Indium-abelled leucocyte scintigraphy in the diagnosis of inflammatory disease—first results

Abstract

In 61 patients, 66 scans were performed with 111In-labelled autologous leucocytes to evaluate this method in the diagnosis of inflammatory disease. All scintigraphic results were compared with clinical, operative or postmortem findings. In the first 20 examinations in 19 patients, 2 scintigrams were true positive, 9 true negative one of false positive, whereas 9 scintigrams had to be considered as false negative. These false negative results were probably due to loss of viability of the labelled cells. A modified labelling technique, in which a much lower concentration of oxine was used, was employed for a further 46 examinations in 42 patients. Twenty-four scintigrams were true positive and 22 true negative, while no false positive or false negative results were observed. These results suggest that this modified technique is useful in the diagnosis of inflammatory disease.

N-(4-18F-Fluorobenzoyl)Interleukin-2 for PET of Human-Activated T Lymphocytes

“…In vivo detection of lymphocytes using nuclear imaging techniques is an important diagnostic tool developed more than 30 y ago with the introduction of labeled autologous cells (14,15). Later, and because of the toxicity of direct radiolabeling on lymphocytes, several peptides and proteins were radiolabeled with SPECT isotopes, including IL1, FAS ligand peptides, and, more recently, antibodies against CD25, CD3, CD20, HLA-DR, and others (3,(16)(17)(18)(19)(20).…”

Labelling of granulocytes with radioindium: Cell isolation and labelling parameters

“…In these suspensions, 86.2 k 11.3% of the cell-bound radioactivity was associated with granulocytes. In the suspension used by others the erythrocyte/ granulocyte ratio has varied between 1/5 and 5 / 1 (10,11,14,18). The results of this study show that a ratio of fewer than 5 erythrocytes per granulocyte is acceptable.…”

“…Most investigators have used mixed leucocyte populations, isolated by dextran or hydroxyethyl starch sedimentation and centrifugation. This simple labelling protocol usually produces suspensions containing 20-50% lymphocytes (10,11) although as little as 5 % lymphocytes has been reported (4), the degree of contamination with lymphocytes being highly dependent on the peripheral differential count.…”

89Zr-leukocyte labelling for cell trafficking: in vitro and preclinical investigations

“…In vivo cell tracking with the use of nuclear medicine imaging techniques has been an important diagnostic tool since it was developed more than 40 years ago with the introduction of labelled autologous cells (Lee et al 2020 ; Gordon and Vivian 1984 ; McAfee and Thakur 1976 ; Rovekamp et al 1981 ; Welling et al 2019 ). Although few PET radioisotopes have been utilized, other methods to label gamma emitters have been widely used.…”