113 The effects of bisphenols on cryopreserved bovine spermatozoa

Davis, Ola S; Hickey, K.; Favetta, Laura A.

doi:10.1071/rdv34n2ab113

Cited by 2 publications

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“…In various animal models, BPS has been shown to disrupt granulosa cell steroidogenesis ( Téteau et al , 2020 , 2023 ), impair the in vitro early developmental competence of oocytes and embryos ( Desmarchais et al , 2020 ; Žalmanová et al , 2023 ) and reduce the motility of spermatozoa ( Torres-Badia et al , 2023 ; Téteau et al , 2023 ). Similarly, BPF has been shown to affect motility, capacitation, and mitochondrial membrane potential in cryopreserved bovine sperm ( Nguyen et al , 2022 ; Davis et al , 2023 ). In our study, none of the included consumables were made of polycarbonates.…”

Section: Discussionmentioning

confidence: 99%

Disposables used cumulatively in routine IVF procedures could display toxicity

Delaroche,

Besnard,

Ouary

et al. 2024

Human Reproduction

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STUDY QUESTION Is there a cumulative toxicity of disposables used in IVF procedures? SUMMARY ANSWER A toxicity may be detected when consumables are used cumulatively, while no toxicity is detected when the same consumables are used and tested individually. WHAT IS KNOWN ALREADY Many components of items used in IVF laboratories may impair human embryonic development. Consequently, it is necessary to screen all reagents and materials which could be in contact with gametes and embryos. Toxicity tests, such as the mouse embryo assay and the human sperm motility assay (HSMA), are used by manufacturers as quality control tools to demonstrate the safety of their products. This evaluation is currently individually performed for each single consumable. However, during an IVF cycle, several devices are used sequentially, potentially creating a cumulative exposure to chemical contaminants, which could not be detected for individually tested consumables. STUDY DESIGN, SIZE, DURATION The objective of this observational study conducted from March 2021 to October 2022 was to evaluate with the HSMA methodology if there was a cumulative toxicity when several disposables are sequentially used. Fourteen categories of consumables currently used in routine IVF procedures were studied, which included devices used for sperm and oocyte collection (cups, condoms, and oocyte aspiration needles), manipulation (flasks, tubes, tips, pipettes, embryo transfer catheters, syringes, and gloves), culture (dishes), and storage (straws). PARTICIPANTS/MATERIALS, SETTING, METHODS After obtaining patient consent, the surplus semen assessed as having normal parameters according to the World Health Organization 2010 criteria were used to perform the HSMAs. First, each consumable was tested individually. Then, associations of three, four, and five consumables, previously validated as non-toxic when tested individually, were analyzed. HSMAs were conducted three times to ensure reproducibility, with a defined toxicity threshold of a sperm motility index (SMI) below 0.85 in at least two of three tests. MAIN RESULTS AND THE ROLE OF CHANCE Thirty-six references of disposables were first individually tested across 53 lots. Forty-nine (92%) demonstrated compliance. However, four (8%) devices revealed toxicity: one lot of 1 ml syringes, two lots of sperm cups, and one lot of 25 cm2 flasks. These four references were excluded from the IVF routine procedures. A total of 48 combinations of consumables were assessed, involving 41 lots from 32 references that were previously individually tested. Among the evaluated combinations, 17 out of 48 (35%) associations exhibited toxicity with a SMI below 0.85 for two of the three tests (n = 8) or all the three tests (n = 9). Notably, three out of 17 (18%) of the three-consumable associations, five out of 16 (31%) of the four-consumable associations, and nine out of 15 (60%) of the five-consumable associations were found not compliant. The toxicity did not originate from a single consumable, because only consumables that were individually pre-validated as non-toxic were included in the combinations, but the toxicity had a cumulative origin. The risk of cumulative toxicity increased with the number of consumables included in the association (Cochran–Mantel–Haenszel statistic, P = 0.013). LIMITATIONS, REASONS FOR CAUTION The high proportion of non-compliant combinations of disposables can be attributed directly to the extreme rigorous extraction conditions employed during the tests, which could deviate from the conditions encountered in routine clinical use. Also, the methodology employed in the HSMAs (e.g. toxicity extraction duration, sperm concentrations, and protein supplementation of the medium) can influence the sensitivity of the tests. WIDER IMPLICATIONS OF THE FINDINGS This study highlights the significance of performing toxicity testing on devices before introducing them into clinical practice. Disposables should be tested individually to detect immediate toxicities and also in combination. Our results advocate rationalizing the number of consumables used in each IVF procedure and re-evaluating the use of glass consumables. STUDY FUNDING/COMPETING INTEREST(S) This study received fundings from GCS Ramsay Santé pour l’Enseignement et la Recherche (Paris, France) and the Centre de Biologie Médicale BIOGROUP (Le Chesnay-Rocquencourt, France). The authors declare that they have no conflict of interest that could be perceived as prejudicing the impartiality of the reported research. TRIAL REGISTRATION NUMBER N/A.

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Section: Discussionmentioning

confidence: 99%

Disposables used cumulatively in routine IVF procedures could display toxicity

Delaroche,

Besnard,

Ouary

et al. 2024

Human Reproduction

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“…In spermatozoa, we observed a significant decrease in progressive motility after treatment with all three bisphenols. Although the experiments here presented have been conducted in technical replicates on an individual bull, we have established that this bull is representative of at least three other bull semen samples previously analyzed ( n = 4) [ 39 ]. Motility is vital to the reproductive potential of the male gamete to fertilize the oocyte as sperm must swim to penetrate both the cumulus layer and the zona pellucida [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

“…We previously established that the bull sample used here showed consistent results under the same experimental conditions as the results obtained on frozen and fresh semen of three other individual bulls of known and proven fertilization capability. In fact, when motility (both by Makler Counter Chamber and the Sperm Class Analyzer-SCA ® CASA System software) and morphology (by microscopy) were analyzed on all four bulls at the same time, no differences were observed among the four bulls investigated in technical triplicates [ 39 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of BPA, BPS, and BPF on Oxidative Stress and Antioxidant Enzyme Expression in Bovine Oocytes and Spermatozoa

Nguyen

Sabry

Davis

et al. 2022

Genes

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Bisphenol A (BPA) and its analogs, bisphenol S (BPS) and bisphenol F (BPF), might impact fertility by altering oxidative stress pathways. Here, we hypothesize that bisphenols-induced oxidative stress is responsible for decreased gamete quality. In both female (cumulus-oocyte-complexes—COCs) and male (spermatozoa), oxidative stress was measured by CM-H2DCFDA assay and key ROS scavengers (SOD1, SOD2, GPX1, GPX4, CAT) were quantified at the mRNA and protein levels using qPCR and Western blot (COCs)/immunofluorescence (sperm). Either gamete was treated in five groups: control, vehicle, and 0.05 mg/mL of BPA, BPS, or BPF. Our results show elevated ROS in BPA-treated COCs but decreased production in BPS- and BPF-treated spermatozoa. Additionally, both mRNA and protein expression of SOD2, GPX1, and GPX4 were decreased in BPA-treated COCs (p < 0.05). In sperm, motility (p < 0.03), but not morphology, was significantly altered by bisphenols. SOD1 mRNA expression was significantly increased, while GPX4 was significantly reduced. These results support BPA’s ability to alter oxidative stress in oocytes and, to a lesser extent, in sperm. However, BPS and BPF likely act through different mechanisms.

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113 The effects of bisphenols on cryopreserved bovine spermatozoa

Cited by 2 publications

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Disposables used cumulatively in routine IVF procedures could display toxicity

Disposables used cumulatively in routine IVF procedures could display toxicity

Effects of BPA, BPS, and BPF on Oxidative Stress and Antioxidant Enzyme Expression in Bovine Oocytes and Spermatozoa

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