113Cd nuclear magnetic resonance of mammalian erythrocyte carbonic anhydrases.

Uiterkamp, A.J.M. Schoot; Armitage, Ian M.; Coleman, Joseph E.

doi:10.1016/s0021-9258(19)85612-0

Cited by 32 publications

(6 citation statements)

References 23 publications

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“…The 113 Cd nucleus has been used successfully in probing the metalloprotein sites containing Cd(II). Among the examples of such molecules are metallothioneins, troponin C, calmodulin, and others . Key to these studies is the chemical shift of 113 Cd, which is particularly sensitive to the nature of the donor atom(s) in the ligand(s) bound to the metal ion, and to the resulting coordination number and geometry.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and Spectroscopic and Structural Studies of a New Cadmium(II)−Citrate Aqueous Complex. Potential Relevance to Cadmium(II)−Citrate Speciation and Links to Cadmium Toxicity

et al. 2003

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The presence of cadmium in the environment undoubtedly contributes to an increased risk of exposure and ultimate toxic influence on humans. In an effort to comprehend the chemical and biological interactions of Cd(II) with physiological ligands, like citric acid, we explored the requisite aqueous chemistry, which afforded the first aqueous Cd(II)-citrate complex [Cd(C(6)H(6)O(7))(H(2)O)](n)() (1). Compound 1 was characterized by elemental analysis, and spectroscopically by FT-IR and (113)Cd MAS NMR. Compound 1 crystallizes in the orthorhombic space group P2(1)2(1)2(1), with a = 6.166(2) A, b = 10.508(3) A, c = 13.599(5) A, V = 881.2(5) A(3), and Z = 4. The X-ray structure of 1 reveals the presence of octahedral Cd(II) ions bound to citrate ligands in a molecular crystal lattice. Citrate acts as a tridentate binder promoting coordination to one Cd(II) through the central alcoholic moiety, one terminal carboxylate group, and the central carboxylate group. In addition, the central carboxylate binds to three Cd(II) ions. Specifically, one of the oxygens of the central carboxylate serves as a bridge to two neighboring Cd(II) ions, while the other oxygen binds to a third Cd(II). A bound water molecule completes the coordination requirements of Cd(II). (113)Cd MAS NMR studies project the spectroscopic signature of the nature of the coordination environment around Cd(II) in 1, thus corroborating the X-ray findings. Collectively, the data at hand are in line with past solution studies. The latter predict that other similar low molecular mass Cd(II)-citrate complexes may exist in the acidic pH region, thus influencing the uptake of cadmium by living (micro)organisms, their ability to metabolize organic substrates, and possibly Cd(II) toxicity.

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Section: Discussionmentioning

confidence: 99%

Synthesis and Spectroscopic and Structural Studies of a New Cadmium(II)−Citrate Aqueous Complex. Potential Relevance to Cadmium(II)−Citrate Speciation and Links to Cadmium Toxicity

et al. 2003

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“…The other unexpected property of the 113Cd derivative of GR is the presence of a third metal binding site containing sulfur ligands (Figure 4C). The flexibility of metal exchange properties of this site are such that the presence of the metal ion at the third site exchange-modulates the chemical shifts of the first two sites with a frequency corresponding to intermediate exchange, [103][104] et al, 1980;Coleman et al, 1979). Under some circumstances, the binding of additional ligands to the protein can damp these conformational modulations such that ll3Cd signals reappear, e.g., binding of to "3Cd carbonic anhydrase (School Uiterkamp et al, 1980) or inorganic phosphate to alkaline phosphatase (Gettins & Coleman, 1983).…”

Section: Discussionmentioning

confidence: 99%

Cadmium-113 NMR studies of the DNA binding domain of the mammalian glucocorticoid receptor

Pan¹,

Freedman²,

Coleman³

1990

Biochemistry

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The DNA binding domain of the mammalian glucocorticoid hormone receptor (GR) contains nine highly conserved cysteine residues, a conservation shared by the superfamily of steroid and thyroid hormone receptors. A fragment [150 amino acids (AA) in length] consisting of GR residues 407-556, containing within it the entire DNA binding domain (residues 440-525), has been overexpressed and purified from Escherichia coli previously. This fragment has been shown to contain 2.3 +/- 0.2 mol of Zn(II) per mole of protein [Freedman, L. P., Luisi, B. F., Korszun, Z. R., Basavappa, R., Sigler, P. B., & Yamamoto, K. R. (1988) Nature 334, 543]. Zn(II) [or Cd(II) substitution] has been shown to be essential for specific DNA binding. 113Cd NMR of a cloned construct containing the minimal DNA binding domain of 86 AA residues [denoted GR(440-525)] with 113Cd(II) substituted for Zn(II) identifies 2 Cd(II) binding sites by the presence of 2 113Cd NMR signals each of which integrates to 1 113Cd nucleus. The chemical shifts of these two sites, 704 and 710 ppm, suggest that each 113Cd(II) is coordinated to four isolated -S- ligands. Shared -S- ligands connecting the two 113Cd(II) ions do not appear to be present, since their T1s differ by 10-fold, 0.2 and 2.0 s, respectively. Addition of a third 113Cd(II) or Zn(II) to 113Cd2GR(440-525) results in occupancy of a third site, which introduces exchange modulation of the two original 113Cd NMR signals causing them to disappear. Addition of EDTA to the protein restores the original two signals.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Ordering information is given on any current masthead page. Received January 27,1983 It has been shown that the equilateral triangular, bi-oxo-capped type of trimolybdenum cluster compound can be obtained by reducing Na2Mo04 in acetic anhydride. Both W(CO)6 and zinc dust have been used as reductants.…”

Section: Introductionmentioning

confidence: 99%

Pentagonal-bipyramid geometry and cadmium-113 NMR chemical shifts: crystal structures and solution and solid-state cadmium-113 NMR of bis(.mu.-o-hydroxybenzoato)bis(o-hydroxybenzoato)tetraaquodicadmium(II) and tris(pyridine)bis(o-hydroxybenzoato)cadmium(II)

Charles¹,

Griffith²,

Rodesiler³

et al. 1983

Inorg. Chem.

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Bis(~-o-hydroxybenzoato)bis(o-hydroxybenzoato)tetraaquodicadmium(II) (I) and tris(pyridine)bis(o-hydroxybenzoat0)-cadmium(I1) (11) have been shown by X-ray diffraction to consist of pentagonal-bipyramidal seven-coordinate Cd(I1). In the former, the coordination sphere is made up of five carboxyl oxygen atoms in the equatorial plane (one bridging) and two oxygen atoms of water molecules in the axial positions. The latter has four carboxyl oxygen atoms and one pyridine nitrogen atom in the equatorial plane and two axial pyridine nitrogen atoms to complete the coordination sphere. The solid-state MAS '%d NMR chemical shifts of these compounds are -31 and +61 ppm, respectively. The lI3Cd NMR chemical shifts of these and other Cd-containing compounds are correlated with the number of lone pairs that are directed toward the Cd atom. The shielded chemical shifts of a number of Cd model compounds as well as Cd-containing macromolecules are discussed. Crystal data (I): a = 7.747 (3) A, b = 12.455 (3) A, c = 15.725 (7) A, p = 96.23 (3)", 2 = 4, P2Ja, pohd = 1.83 (2) g/cm3, pcald = 1.86 g/cm3, N O = 5058, NV = 208, R , = 0.028. Crystal data (11): a = g/cm3, N O = 4944, NV = 428, RF = 0.029. For both I and 11, structures were refined by full-matrix least squares with anisotropic temperature factors and absorption corrections. 10.187 (4) A, b = 14.846 (5) A, C = 17.965 (5) A, p = 93.79 (3)", z = 4, P2l/ll, pobsd = 1.54 (2) g/Cm3, pcalcd = 1.53

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113Cd nuclear magnetic resonance of mammalian erythrocyte carbonic anhydrases.

Cited by 32 publications

References 23 publications

Synthesis and Spectroscopic and Structural Studies of a New Cadmium(II)−Citrate Aqueous Complex. Potential Relevance to Cadmium(II)−Citrate Speciation and Links to Cadmium Toxicity

Synthesis and Spectroscopic and Structural Studies of a New Cadmium(II)−Citrate Aqueous Complex. Potential Relevance to Cadmium(II)−Citrate Speciation and Links to Cadmium Toxicity

Cadmium-113 NMR studies of the DNA binding domain of the mammalian glucocorticoid receptor

Pentagonal-bipyramid geometry and cadmium-113 NMR chemical shifts: crystal structures and solution and solid-state cadmium-113 NMR of bis(.mu.-o-hydroxybenzoato)bis(o-hydroxybenzoato)tetraaquodicadmium(II) and tris(pyridine)bis(o-hydroxybenzoato)cadmium(II)

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