119 NO-synthase inductible-2 (NOS2) and vascular endothelial growth factor (VEGF) polymorphisms in systemic lupus erythematosus among algerian patients

Abstract

BackgroundThe development of Systemic Lupus Erythematosus (SLE) depends inter alia on genetic factors including genes involved in oxidative stress and angiogenesis as NOS2 and VEGF. The aim of our study is to evaluate the Single Nucleotide Polymorphisms (SNPs) influence of NOS2 gene (rs2779248, rs2779251 and rs8078340) and VEGF gene (rs1570360 and rs2010963) on SLE development in Algerian patients.MethodsThis is a case-control study of 157 SLE patients (age: 37±2 years, sex ratio: 1: 10, disease duration: 7.6±4.3 years, SLEDAI: 7.3±6.1) and 173 healthy controls (age: 28±9 years, sex ratio: 1: 7). We performed NOS2 and VEGF genes SNPs genotyping TaqMAN technology and we respected the Hardy-Weinberg equilibrium.ResultsFirst, we analyzed the allele frequency of NOS2 and VEGF genes SNPs and we obtained for:NOS2 gene that rs2779248 T allele is associated to SLE’s development (OR 1.92) as well as rs2779251 T (OR 2.01) and rs8078340 A alleles (OR 5.00) unlike rs2779248 C, rs2779251 G and rs8078340 G alleles that are protective against SLE development (OR 0.52, 0.50 and 0.20).VEGF gene that rs2010963 C allele is associated SLE’s development (OR 1.86) unlike rs2010963 G allele that is protective (OR 0.54).Thereafter, we analyzed the genotype frequency and we got for:NOS2 gene that rs2779248 CT genotype is associated to SLE’s development (OR 2.01) as well as rs2779251 GG (OR 1.62) and rs8078340 AA genotypes (OR 3.41) inversely to rs2779248 CC, rs2779251 TT and rs8078340 GG genotypes that are protective (OR 0.35, 0.24 and 0.20).VEGF gene that rs1570360 GG genotype is associated to SLE’s development (OR 1.73) as well as rs2010963 CC genotype (OR 2.91) unlike rs1570360 AG and rs2010963 GG genotypes that are protective (OR 0.51 and 0.58).Furthermore, we observed that the VEGF rs1570360 G allele was assiociated to lupus nephritis development (OR 3.51) as well as GG genotype (OR 3.82). Regarding the haplotype analysis, it showed for the NOS2 gene that AGG haplotype is associated to SLE’s development (OR 2.12) and that CGG is protective (OR 0.50). Finally, as a whole, our results are consistent with the literature data.ConclusionsAt the end of our study, we have demonstrated the role the NOS2 and VEGF genes SNPs in the genetic susceptibility to develop SLE in Algerian patients.Funding Source(s):Pasteur Institute of Algeria

Oral Ulceration in Systemic Lupus Erythematosus in Iraqi Patients and the Levels of Serum Vascular Endothelial Growth Factor-A

“…The results of this study showed that female to male ratio was 12:1in SLE, these results agree with others [22] in a large cohorts study conducted in UK with a total of 924 SLE patients were involved found the ratio was 13;1 ,A study done by [23] in Cairo Egypt of 60 SLE patients male was 4(6.66%) and female was 56(93.33%), other found women are estimated to be six to ten times more likely to develop SLE than man [24] , finally a study done in Algeria found female to male ratio: 10: 1 [25] . These differences between female to male in the incidence of disease may be related to the hormonal role of estrogen and other gonadal hormones which have complex effects on the immune system, also the disease activity increases during pregnancy, a role for female hormones has been proposed.…”