[12] Vernonia lipase: A plant lipase with strong fatty acid selectivity

Adlercreutz, Patrick; Gitlesen, Thomas; Ncube, Ignatious; Read, John S.

doi:10.1016/s0076-6879(97)84014-1

Cited by 12 publications

(11 citation statements)

References 19 publications

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“…Strain variability could also explain the wide variability in enzyme production under solid state fermentation. The differences in esterase activities found in solid state fermentations versus culture broth could be ascribed to the interfacial requirements of esterases or to the optimal water activity requirements we see in the use of these enzymes in organic solvents (Adlercreutz et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Esterases and putative lipases from tropical isolates of Aureobasidium pullulans

Kudanga

Mwenje

Mandivenga

et al. 2007

J. Basic Microbiol.

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Esterases and lipases have been studied in a number of fungi, though very little is known about esterases from Aureobasidium pullulans especially from the African tropics. In this study, forty-two Zimbabwean isolates were screened for lipase activity on tributyrin agar. Extracellular esterase activities of seven selected isolates were studied under varying conditions using para-nitrophenol acetate as substrate. Twenty isolates (48%) showed lipolytic activity; sixteen showed negative results for lipase activity while the rest showed weak activities. Esterase activities in broth cultures ranged from 0.011-0.223 mmol/microg protein/min while activities ranged from 1.5-12.8 U/ml under solid state fermentation. The esterases were optimally active at pH 7.6-8.0, showed a temperature optimum of 35 degrees C and retained more than 50% activity at temperatures up to 60 degrees C and at pH 4.0-7.0 after 150 min. Enzyme production was optimal after 5-6 days with diammonium hydrogen phosphate as nitrogen source. Isolates showed variations in preference for carbon source for esterase production. The A. pullulans esterases differed from most fungal esterases in that they are optimally active in alkaline conditions and are active over a broad pH range.

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Section: Discussionmentioning

confidence: 99%

Esterases and putative lipases from tropical isolates of Aureobasidium pullulans

Kudanga

Mwenje

Mandivenga

et al. 2007

J. Basic Microbiol.

Self Cite

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“…Animal lipases are extracted, for the most part, from the pancreas of pigs and sheep, but they are also isolated from the liver, stomach and intestinal wall. In plants, lipases are located in seeds and vegetative organs [18]. However, most lipases that are studied and used industrially are obtained from microbial sources, such as bacteria, yeast and fungi, due to their unique properties, such as their superior stability, selectivity and substrate specificity position.…”

Section: Structure and Specificity Of Lipasesmentioning

confidence: 99%

“…Agricultural wastes include stems, leaves, seed pods, husks, seeds, roots, bagasse and molasses. Industrial wastes mainly consist of the waste produced by the food processing industries, and includes peels, oil cakes and waste water [18]. The food and agricultural industry is expanding rapidly.…”

Section: Agro-industrial Residues and Wastes For Lipase Productionmentioning

confidence: 99%

Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

et al. 2021

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Lipases are enzymes that catalyze various types of reactions and have versatile applications. Additionally, lipases are the most widely used class of enzymes in biotechnology and organic chemistry. Lipases can be produced by a wide range of organisms including animals, plants and microorganisms. Microbial lipases are more stable, they have substrate specificity and a lower production cost as compared to other sources of these enzymes. Although commercially available lipases are widely used as biocatalysts, there are still many challenges concerning the production of microbial lipases with the use of renewable sources as the main component of microbial growth medium such as straw, bran, oil cakes and industrial effluents. Submerged fermentation (SmF) and solid-state fermentation (SSF) are the two important technologies for the production of lipases by microorganisms. Therefore, this review focuses on microbial lipases, especially their function, specificity, types and technology production, including the use of renewable agro-industrial residues and waste materials.

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“…Während der Keimung werden TAGs mit hoher Selektivität durch Triacylglyceridlipasen (TGLs), die ebenfalls mit der Membran der Ölkörper assoziiert sind, zu Glycerin und freien Fettsäuren hydrolisiert (Adlercreutz et al, 1997) produzierenden Oxidasen und Katalasen, welche das gebildete H 2 O 2 sofort abbauen (Purdue und Lazarow, 2001).…”

Section: Einleitung 1 Lipid-mobilisierung Während Der Keimungsentwick...unclassified

Fettsäuretransport in die peroxisomale Matrix von <i>Arabidopsis thaliana</i>

Annett¹

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Aqua destillata ATP Adenosintriphosphat bp Basenpaar BSA engl. Bovine serum albumin bzw. beziehungsweise °C Grad Celsius Carb Carbenicillin clonNAT Nourseothricin cDNA engl. complementary DNA Ci Curie CoA Coenzym A C-Terminus Carboxy-Terminus DMSO Dimethylsulfoxid DNA engl. desoxyribonucleic acid = Desoxyribonukleinsäure dNTP Desoxyribonukleosidtriphosphat DTT Dithiothreitol EDTA Etylendiamintetraessigsäure EYFP engl. enhanced yellow fluorescence protein Volt VLCFA engl. Very long chain fatty acid = sehr langkettige Fettsäure Vol. Volumen (v/v) Volumen pro Volumen vs. lat. versus = gegen WT Wildtyp w/v engl. Weight per volume = Gewicht pro Volumen

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[12] Vernonia lipase: A plant lipase with strong fatty acid selectivity

Cited by 12 publications

References 19 publications

Esterases and putative lipases from tropical isolates of Aureobasidium pullulans

Esterases and putative lipases from tropical isolates of Aureobasidium pullulans

Various Perspectives on Microbial Lipase Production Using Agri-Food Waste and Renewable Products

Fettsäuretransport in die peroxisomale Matrix von <i>Arabidopsis thaliana</i>

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