12. Zinc Fingers

Isernia, Carla; Malgieri, Gaetano; Russo, Luigi; D’Abrosca, Gianluca; Baglivo, Ilaria; Pedone, Paolo V.; Fattorusso, Roberto

doi:10.1515/9783110589757-018

Cited by 11 publications

(11 citation statements)

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“…For instance, histidine contains nitrogen atoms that are part of a heterocyclic aromatic ring and which therefore possess unique metal-binding properties amongst the class of amino acids. Inspired by the wide occurrence of the [His 2 ZnCys 2 ] binding motif in zinc finger proteins, − the affinities of Zn II centers for histidine have been exploited in the design of chemosensors in a biometric fashion. The chemosensors discussed within this section are summarized in Table .…”

Section: Amino Acidsmentioning

confidence: 99%

Molecular Probes, Chemosensors, and Nanosensors for Optical Detection of Biorelevant Molecules and Ions in Aqueous Media and Biofluids

et al. 2022

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Synthetic molecular probes, chemosensors, and nanosensors used in combination with innovative assay protocols hold great potential for the development of robust, low-cost, and fast-responding sensors that are applicable in biofluids (urine, blood, and saliva). Particularly, the development of sensors for metabolites, neurotransmitters, drugs, and inorganic ions is highly desirable due to a lack of suitable biosensors. In addition, the monitoring and analysis of metabolic and signaling networks in cells and organisms by optical probes and chemosensors is becoming increasingly important in molecular biology and medicine. Thus, new perspectives for personalized diagnostics, theranostics, and biochemical/ medical research will be unlocked when standing limitations of artificial binders and receptors are overcome. In this review, we survey synthetic sensing systems that have promising (future) application potential for the detection of small molecules, cations, and anions in aqueous media and biofluids. Special attention was given to sensing systems that provide a readily measurable optical signal through dynamic covalent chemistry, supramolecular host− guest interactions, or nanoparticles featuring plasmonic effects. This review shall also enable the reader to evaluate the current performance of molecular probes, chemosensors, and nanosensors in terms of sensitivity and selectivity with respect to practical requirement, and thereby inspiring new ideas for the development of further advanced systems.

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Section: Amino Acidsmentioning

confidence: 99%

Molecular Probes, Chemosensors, and Nanosensors for Optical Detection of Biorelevant Molecules and Ions in Aqueous Media and Biofluids

et al. 2022

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“…The consensus sequence (Y/F)-X-C-X 2,4 -C-X 3 -X-L-X 2 -H-X 3,4 -H consists of ~30 amino acids with a CCHH coordination sphere and a three residue hydrophobic core. Recently, larger βββαα zinc-binding globular domains, involved in DNA-binding and genes regulation, were also found in Prokaryotes [ 48 , 49 , 50 ].…”

Section: Zinc Fingersmentioning

confidence: 99%

Host and Viral Zinc-Finger Proteins in COVID-19

Esposito

D’Abrosca

Antolak

et al. 2022

IJMS

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An unprecedented effort to tackle the ongoing COVID-19 pandemic has characterized the activity of the global scientific community over the last two years. Hundreds of published studies have focused on the comprehension of the immune response to the virus and on the definition of the functional role of SARS-CoV-2 proteins. Proteins containing zinc fingers, both belonging to SARS-CoV-2 or to the host, play critical roles in COVID-19 participating in antiviral defenses and regulation of viral life cycle. Differentially expressed zinc finger proteins and their distinct activities could thus be important in determining the severity of the disease and represent important targets for drug development. Therefore, we here review the mechanisms of action of host and viral zinc finger proteins in COVID-19 as a contribution to the comprehension of the disease and also highlight strategies for therapeutic developments.

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“…Among them was TRIM proteins, which have three types of domains at their N -terminus: RING finger domain, B-box zinc finger domain, and coiled-coil region. These domains provide the involvement of TRIM proteins transcriptional regulation, cytoskeletal organization, epithelial development, cell adhesion, and immune response [ 22 ]. Another retrieved transcription factor, HHEX, is involved in cell growth and differentiation, hepatic and pancreatic development, and anterior–posterior pattern specification via the Wnt signaling pathway [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Short Linear Motifs Orchestrate Functioning of Human Proteins during Embryonic Development, Redox Regulation, and Cancer

et al. 2022

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Short linear motifs (SLiMs) are evolutionarily conserved functional modules of proteins that represent amino acid stretches composed of 3 to 10 residues. The biological activities of two short peptide segments of human alpha-fetoprotein (AFP), a major embryo-specific and cancer-related protein, have been confirmed experimentally. This is a heptapeptide segment LDSYQCT in domain I designated as AFP14–20 and a nonapeptide segment EMTPVNPGV in domain III designated as GIP-9. In our work, we searched the UniprotKB database for human proteins that contain SLiMs with sequence similarity to the both segments of human AFP and undertook gene ontology (GO)-based functional categorization of retrieved proteins. Gene set enrichment analysis included GO terms for biological process, molecular function, metabolic pathway, KEGG pathway, and protein–protein interaction (PPI) categories. We identified the SLiMs of interest in a variety of non-homologous proteins involved in multiple cellular processes underlying embryonic development, cancer progression, and, unexpectedly, the regulation of redox homeostasis. These included transcription factors, cell adhesion proteins, ubiquitin-activating and conjugating enzymes, cell signaling proteins, and oxidoreductase enzymes. They function by regulating cell proliferation and differentiation, cell cycle, DNA replication/repair/recombination, metabolism, immune/inflammatory response, and apoptosis. In addition to the retrieved genes, new interacting genes were identified. Our data support the hypothesis that conserved SLiMs are incorporated into non-homologous proteins to serve as functional blocks for their orchestrated functioning.

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12. Zinc Fingers

Cited by 11 publications

References 0 publications

Molecular Probes, Chemosensors, and Nanosensors for Optical Detection of Biorelevant Molecules and Ions in Aqueous Media and Biofluids

Molecular Probes, Chemosensors, and Nanosensors for Optical Detection of Biorelevant Molecules and Ions in Aqueous Media and Biofluids

Host and Viral Zinc-Finger Proteins in COVID-19

Short Linear Motifs Orchestrate Functioning of Human Proteins during Embryonic Development, Redox Regulation, and Cancer

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