1204 Enhancing NK cell function in the ‘cold’ tumor microenvironment of prostate cancer with a novel tri-specific Killer Engager against prostate-specific membrane antigen (PSMA)

Abstract

1202 Figure 1 Structure of a PSMA TriKE molecule. A PSMA TriKE molecule consists of a humanized anti-CD16 single domain antibody (sdAb) derived from camelid, an anti-PSMA single chain variable fragment (scFv) and an IL-15 molecule. IL-15, interleukin-15; mCRPC, metastatic castration-resistant prostate cancer; PSMA, prostatespecific membrane antigen; TriKE, tri-specific killer engager Abstract 1202 Figure 2 PSMA TriKE relieves suppressed NK cell cytotoxicity induced by hypoxia. NK cells were incubated with 0.06nM IL-15 or 3nM PSMA TriKE (at equifunctional concentrations) in normoxia (20% oxygen) or hypoxia (1% oxygen) for 7 days then harvested and plated at effectors to targets (E:T) ratio of 2:1 for 48 hours in normoxia to assess NK cell cytotoxicity against C4-2 using IncuCyte live cell imaging. Representative data of N=5.