13,16-Dimethyl Octacosanedioic Acid (
            <i>iso</i>
            -Diabolic Acid), a Common Membrane-Spanning Lipid of Acidobacteria Subdivisions 1 and 3

Damsté, Jaap S. Sinninghe; Rijpstra, W. Irene C.; Hopmans, Ellen C.; Weijers, Johan W. H.; Foesel, Bärbel U.; Overmann, Jörg; Dedysh, Svetlana N.

doi:10.1128/aem.00466-11

Cited by 387 publications

(224 citation statements)

References 60 publications

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“…Alternatively, GDGT-Ia-IIIa derive from the mat-forming bacteria, possibly from anaerobic Acidobacteria indigenous to the mats (Weijers et al, 2009(Weijers et al, , 2010Sinninghe Damsté et al, 2011). This is consistent with in situ production of branched GDGTs in other marine sediments (e.g.…”

Section: Gdgtssupporting

confidence: 58%

Biogeochemistry of intertidal microbial mats from Qatar: New insights from organic matter characterisation

Słowakiewicz

Whitaker

Thomas

et al. 2016

Organic Geochemistry

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms AbstractUnderstanding early organic matter alteration and preservation in marine carbonate-evaporite systems could improve understanding of carbon cycling and hydrocarbon source rock prediction in such environments. It is possible that organic-rich microbial mats are important contributors to preserved hydrocarbons, and to explore this we examined changes in lipid composition in such a mat from a mesohaline intertidal lagoon, eastern Qatar. The mat reaches > 5 cm thickness over a carbonate mud substrate, rich in seagrass, gastropods and other small bioclasts. Clear lamination, with distinct downward colour changes from green to pink to brown, reflects different microbial mat communities. The layers contain spheroids of probable dolomite, the precipitation of which was plausibly bacterially-mediated. Lipids [n-alkanes, fatty acids (FAs), hopanoids, isoprenoid hydrocarbons, dialkyl glycerol diethers (DAGEs), and isoprenoid (0 to 4) and branched (Ia to IIIa) GDGTs] reflect the diverse mat-building phototrophic, heterotrophic and chemoorganotrophic microorganisms, as well as some likely allochthonous material (i.e. steroids, n-alkanols, high molecular weight nalkanes). The lipids clearly document the change in microbial community, with phytene being the predominant hydrocarbon in the phototrophic surface layer. Oxygen and pH drop significantly 0.2 cm below the mat surface, coincident with the predominance of Deltaproteobacteria and increased concentrations of archaeal and bacterial glycerol dialkyl glycerol tetraether lipids andC 15 /C 16 and C 16 /C 17 dialkyl glycerol ether lipids in the deeper layers. Archaeol, likely of methanogen origin, is most abundant in the deepest layer. Allochthonous inputs occur throughout the mat, including abundant steroids, especially dinosterol and dinostanol, that are possibly related to periodic algal (dinoflagellate) blooms in the Arabian Gulf. Both n-alkanes and n-alkanols appear to be derived from seagrass. Organic matter contents decrease in the deepest mat; this suggests an overall low preservation potential of OM in intertidal mesohaline-hypersaline mats of the Arabian Gulf, which in turn suggests that these are not the major source of hydrocarbons in microbially-dominated carbonateevaporite systems.2

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Section: Gdgtssupporting

confidence: 58%

Biogeochemistry of intertidal microbial mats from Qatar: New insights from organic matter characterisation

Słowakiewicz

Whitaker

Thomas

et al. 2016

Organic Geochemistry

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“…1) are membrane lipids produced by unknown bacteria, even though some might belong to the phylum Acidobacteria (Weijers et al, 2009;Sinninghe Damsté et al, 2011). The relative distribution has been shown to depend on environmental parameters; the degree of methylation, expressed in the MBT index, was shown to correlate with MAAT and to a lesser extent with soil pH, whereas the relative abundance of cyclopentyl rings, expressed in the CBT index, was observed to depend on soil pH (Weijers et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

A climatic chamber experiment to test the short term effect of increasing temperature on branched GDGT distribution in Sphagnum peat

Huguet

Francez

Jusselme

et al. 2014

Organic Geochemistry

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To cite this version:Arnaud Huguet, Andre-Jean Francez, My Dung Jusselme, Céline Fosse, Sylvie Derenne. A climatic chamber experiment to test the short term effect of increasing temperature on branched GDGT distribution in Sphagnum peat. Organic Geochemistry, Elsevier, 2014, 73, pp.109-112 by unknown Bacteria and are being increasingly used as temperature proxies. Nevertheless, the direct effect of temperature on br GDGT distributions has been rarely evaluated. In this study, the impact of increasing temperature on brGDGT distributions and the speed of adaptation of br GDGT source microorganisms to temperature change were investigated by analysing br GDGTs in Sphagnum peat cores incubated under controlled conditions at two different temperatures -12 and 15 °C -for 1 yr. Br GDGTs present as intact polar lipids (IPLs, presumably derived from recently active microorganims) and core lipids (CLs, derived from dead biomass) were analysed. There were no significant differences in the relative abundances of the most abundant br GDGTs in the CL and IPL pools after 3 months' incubation. In contrast, the distribution of the br GDGT IPLs was significantly affected by temperature after 1 yr, whereas no change was observed in the CL fraction. This suggests that (i) the CL pool of br GDGTs has a turnover of at least 1 yr in peat and (ii) br GDGT source microorganisms rapidly adjust their membrane lipid composition (in > 3 months and < 1 yr, based on IPL analysis). * Corresponding author. Tel: + 33-144-275-172; fax: +33-144-275-150.E-mail address: arnaud.huguet@upmc.fr (A. Huguet). 2 IntroductionPeatlands contain 20-30% of the world's soil C stock, even though they cover only 3-5% of the land area (Gorham, 1991). With respect to global climate change, how a moderate temperature rise would affect the organic matter (OM) pool in peat soils and consequently the long term carbon sequestration potential is not known. Peatlands may change from a C sink function to a C source function. Within the framework of the French ANR project "PEATWARM", the temperature of a pristine Sphagnum-dominated peatland (Frasne, French Jura Mountains) was experimentally increased. The aims were to (i) investigate the impact of a moderate temperature rise on biotic and abiotic compartments of this ecosystem and (ii) more generally determine to which extent this temperature change could modify the C sink function of temperate peatlands.We specifically investigated the effect of in situ experimental microclimate warming (+ 1.2 °C, mean annual air temperature, MAAT) on branched glycerol dialkyl glycerol tetraether (br GDGT) distribution in the Frasne peatland (Huguet et al., 2013); Br GDGTs ( Fig. 1) are membrane lipids produced by unknown bacteria, even though some might belong to the phylum Acidobacteria (Weijers et al., 2009;Sinninghe Damsté et al., 2011). The relative distribution has been shown to depend on environmental parameters; the degree of methylation, expressed in the MBT index, was shown to correlate with MAAT and to a lesser extent with soil p...

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“…Lipids were analysed after acid hydrolysis of whole cells, following the procedure described by Sinninghe Damsté et al (2011). The major fatty acids detected in strain PX4 T and PT1 were nC 16 : 0 , nC 18 : 1 v9 and nC 18 : 0 (Table 1).…”

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confidence: 99%

Paludisphaera borealis gen. nov., sp. nov., a hydrolytic planctomycete from northern wetlands, and proposal of Isosphaeraceae fam. nov.

Kulichevskaya

Ivanova

Suzina

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Paludisphaera borealis gen. nov., sp. nov., a hydrolytic planctomycete from northern wetlands, and proposal of Isosphaeraceae fam. nov. Two isolates of aerobic, budding, pink-pigmented bacteria, designated strains PX4 T and PT1, were isolated from a boreal Sphagnum peat bog and a forested tundra wetland. Cells of these strains were non-motile spheres that occurred singly or in short chains. Novel isolates were capable of growth at pH values between 3.5 and 6.5 (optimum at pH 5.0-5.5) and at temperatures between 6 and 30 8C (optimum at 15-25 8C). Most sugars and a number of polysaccharides including pectin, xylan, lichenin and Phytagel were used as growth substrates. The major fatty acids were C 16 : 0 , C 18 : 1 v9 and C 18 : 0 ; the major polar lipids were phosphocholine and trimethylornithine. The quinone was menaquinone-6, and the G+C content of the DNA was 66 mol%. Strains PX4 T and PT1 were members of the order Planctomycetales and displayed 93-94 % 16S rRNA gene sequence similarity to Aquisphaera giovannonii, 91-92 % to species of the genus Singulisphaera and 90-91 % to Isosphaera pallida. The two novel strains, however, differed from members of these genera by cell morphology, substrate utilization pattern and a number of physiological characteristics. Based on these data, the novel isolates should be considered as representing a novel genus and species of planctomycetes, for which the name Paludisphaera borealis gen. nov., sp. nov., is proposed. The type strain is PX4 T (5DSM 28747 T 5VKM B-2904 T ). We also suggest the establishment of a novel family, Isosphaeraceae fam. nov., to accommodate stalk-free planctomycetes with spherical cells, which can be assembled in short chains, long filaments or shapeless aggregates. This family includes the genera Isosphaera, Aquisphaera, Singulisphaera and Paludisphaera.

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13,16-Dimethyl Octacosanedioic Acid ( iso -Diabolic Acid), a Common Membrane-Spanning Lipid of Acidobacteria Subdivisions 1 and 3

Cited by 387 publications

References 60 publications

Biogeochemistry of intertidal microbial mats from Qatar: New insights from organic matter characterisation

Biogeochemistry of intertidal microbial mats from Qatar: New insights from organic matter characterisation

A climatic chamber experiment to test the short term effect of increasing temperature on branched GDGT distribution in Sphagnum peat

Paludisphaera borealis gen. nov., sp. nov., a hydrolytic planctomycete from northern wetlands, and proposal of Isosphaeraceae fam. nov.

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