1353 Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes

Abstract

In this study, amino acid functionalized poly(2-hydroxyethyl methacrylate-N-methacrylolyl-l-phenylalanine) [PHEMAPA] cryogel discs were prepared. In this respect, phenylalanine containing N-methacryloyl-(L)-phenylalanine methyl ester (MAPA) was polymerized with 2-hydroxyethyl methacrylate (HEMA) without requirement of any activation step. Laccase bound poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-phenylalanine) [Lac-PHEMAPA] cryogel discs were applied for decolorization of Reactive Blue-247 (RB-247). The ability of Lac-PHEMAPA cryogel discs on dye decolorization was found to be as 90% in 2 h and even more within 4h. The decolorization activities of 86% and 73% were observed at relatively low (4°C) and high (60°C) temperatures, respectively. The effect of dye concentration on dye decolorization and 100% decolorization activity was achieved in dye concentration between 50–300 ppm. Lac-PHEMAPA cryogel discs maintained 80% of its decolorization activity after six cycles. Consequently, the PHEMAPA cryogel discs are promising materials for immobilizing laccase. The Lac-PHEMAPA has a rapid dye decolorization in a broad range of temperature. The preparation is furthermore very stable and activity is preserved during storage.

Evaluation of reproducible cryogel preparation based on automated image analysis using deep learning

“…1 Due to their unique properties, for example, high porosity, mechanical stability and permeability provided by an interconnected macropore network, polymeric cryogels represent an outstanding class of materials. They can be produced in various shapes, including monoliths, 2-4 beads, 5,6 disks, [7][8][9][10][11] and microspheres. 12,13 Cryogels have been used in a variety of applications where permeability plays an important role, for example, tissue engineering, [14][15][16] building of bioreactors, [17][18][19] immobilizing biomolecules, [20][21][22] capturing target molecules, 23,24 or delivering drugs in a controlled manner.…”

Metal ion‐mediated immobilization of catalase on poly(ε‐caprolactone)/polyethyleneimine electrospun nanofibers

“…9 Various methods have been devised for enzyme immobilization, such as encapsulation, entrapment in a polymer structure, covalent binding to an insoluble carrier, adsorption on insoluble material, and cross-linking using a bifunctional reagent. [10][11][12] Metal ion-mediated enzyme immobilization is one of the most effective approaches among them since the coordination bonds, which are created between metal ions and the enzyme molecules, can effectively prevent the inactivation and denaturation of the enzyme while providing enough binding strength for immobilization. [13][14][15] The majority of transition metal ions are capable of forming coordinate bonds with molecules having S, O, and N. 16 By this means, metal ion-mediated immobilization of enzymes is based on multipoint interactions between the metal ions and the imidazole, indole, and thiol groups of histidine, tryptophan, and cysteine, respectively.…”

“…In addition, in practical applications, the multipoint binding of enzymes to the support helps limit the unfavorable conformational change of enzymes 9 . Various methods have been devised for enzyme immobilization, such as encapsulation, entrapment in a polymer structure, covalent binding to an insoluble carrier, adsorption on insoluble material, and cross‐linking using a bifunctional reagent 10–12 . Metal ion‐mediated enzyme immobilization is one of the most effective approaches among them since the coordination bonds, which are created between metal ions and the enzyme molecules, can effectively prevent the inactivation and denaturation of the enzyme while providing enough binding strength for immobilization 13–15 .…”